PF3D7_1419100 (DDX55)

Genome location: Pf3D7_14_v3:793,391..797,397(+)

Genome classification: Core

Function and Localization

Product Description: ATP-dependent RNA helicase DDX55

SignalP Peptide: N/A

# Transmembrane Domains: 0

EC Numbers: 3.6.4.13 (RNA helicase)

Curated GO (PlasmoDB):

Type	GO Term	Name
Component	GO:0005737	cytoplasm
Component	GO:0005730	nucleolus
Component	GO:0005634	nucleus
Function	GO:0043138	3'-5' DNA helicase activity
Function	GO:0008094	ATP-dependent activity, acting on DNA
Function	GO:0008186	ATP-dependent activity, acting on RNA
Function	GO:0003724	RNA helicase activity

Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):

Stage	LR class	MCA mean	MCA prop. zeros
Sporozoite	possibly expressed	N/A	N/A
Ring	expressed	1.26	0.49
Trophozoite	expressed	0.88	0.55
Schizont	possibly expressed	0.34	0.80
Gametocyte	expressed	0.58	0.69

More info:

Resistome Mutations

Old (Pf3D7v3) Gene ID: PF3D7_1419100

Resistome Missense Mutations: N349Y

Resistome Compounds with Missense Mutations: Lopinavir

Resistome # Samples with Disruptive Mutations: 1 (1 missense, 0 "interesting" missense)

Essentiality (ABS)

Zhang Phenotype: Mutable in CDS

MIS: 1 | MFS: -2.543 | #Insertions: 4

PlasmoGEM Phenotype: Dispensable (Pb ortholog: PBANKA_1024100)

Relative Growth Rate: 0.89 ± 0.15
Confidence: 5.22

RMgmDB ABS Phenotype: Different from wild type (Pb ortholog: PBANKA_1024100)

Modification: Disrupted | RMgm-2832

More info: PhenoPlasm Link

Binding Evidence

AlphaFill Uniprot ID: Q8ILQ9

"Best" AlphaFill ligand hit: ANP (phosphoaminophosphonic acid-adenylate ester, Local RMSD=0.05) with 3B7G (Global RMSD=4.06)

BRENDA EC Inhibitors:

EC #	Name	EC Inhibitors
3.6.4.13	RNA helicase	ATPADPUDPAMPGTPGDPUTPKClNEMCDPITPTDP...

No evidence of orthology to BindingDB entries

Orthology Information

Ortholog Group (OrthoMCL): OG6_532307

No human ortholog(s)

Genetic Variation

MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:

Homozygous genotype calls only

variant type	common	rare	doubleton	singleton
synonymous	6	63	46	67
disruptive	39	176	96	217
missense	22	156	84	184

Any inclusion in genotype call

variant type	common	rare	doubleton	singleton
synonymous	17	131	39	73
disruptive	88	388	155	353
missense	50	311	127	237

PlasmoDB Total SNPs: 141

Non-coding: 34 | Synonymous: 46 | Nonsynonymous: 61 | Stop Codon: 0

Protein Information

Protein Length: 1041 | Molecular Weight (kDa): 124.794

UniProt ID(s): A0A346QRV2, A0A6B9MP50, A7U5X4, Q8ILQ9

PDB ID(s): None

Isoelectric Point: 10.09

Protein Domain Annotations:

Source	Family ID	Description
InterPro	IPR001650	Helicase, C-terminal
InterPro	IPR011545	DEAD/DEAH box helicase domain
InterPro	IPR014001	Helicase superfamily 1/2, ATP-binding domain
InterPro	IPR025313	Domain of unknown function DUF4217
InterPro	IPR027417	P-loop containing nucleoside triphosphate hydrolase
PFam	PF00270	DEAD/DEAH box helicase domain
PFam	PF00271	Helicase, C-terminal
PFam	PF13959	Domain of unknown function DUF4217
Superfamily	SSF52540	P-loop containing nucleoside triphosphate hydrolase

PMID	Title	Authors	DOI/Link
12368864	Genome sequence of the human malaria parasite Plasmodium falciparum.	Gardner MJ, Hall N, ..., Barrell B	10.1038/nature01097
32125511	Plasmodium falciparum DDX55 is a nucleocytoplasmic protein and a 3'-5'direction-specific DNA helicase.	Yasmin R, Kaur I, Tuteja R	10.1007/s00709-020-01495-z