Product Description: glutathione S-transferase
SignalP Peptide: N/A
# Transmembrane Domains: 0
EC Numbers: 2.5.1.18 (Glutathione transferase)
Curated GO (PlasmoDB):
| Type | GO Term | Name |
|---|---|---|
| Function | GO:0004364 | glutathione transferase activity |
| Process | GO:0006749 | glutathione metabolic process |
| Process | GO:0009410 | response to xenobiotic stimulus |
Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):
| Stage | LR class | MCA mean | MCA prop. zeros |
|---|---|---|---|
| Sporozoite | expressed | N/A | N/A |
| Ring | expressed | 0.07 | 0.96 |
| Trophozoite | expressed | 0.22 | 0.86 |
| Schizont | expressed | 0.01 | 0.99 |
| Gametocyte | expressed | 0.06 | 0.96 |
More info:
Old (Pf3D7v3) Gene ID: PF3D7_1419300
Resistome Missense Mutations: None
Resistome Compounds with Missense Mutations: None
Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)
Zhang Phenotype: Non - Mutable in CDS
MIS: 0.119 | MFS: -3.204 | #Insertions: 0
PlasmoGEM Phenotype: Dispensable (Pb ortholog: PBANKA_1023900)
RMgmDB ABS Phenotype: Different from wild type (Pb ortholog: PBANKA_1023900)
Modification: Disrupted | RMgm-2830
More info: PhenoPlasm Link
AlphaFill Uniprot ID: Q8MU52
"Best" AlphaFill ligand hit: PGO (s-1,2-propanediol, Local RMSD=0.03) with 3VPQ (Global RMSD=5.41)
BRENDA EC Inhibitors:
| EC # | Name | EC Inhibitors |
|---|---|---|
| 2.5.1.18 | glutathione transferase | ADPKClNEMSDSGSHDTTEDTAGSSGDTNBNH4ClFeCl3hemin... |
Orthology to BindingDB Entries:
| UniProt | Protein Name | Species | Homology Source | BindingDB Ligands |
|---|---|---|---|---|
| O60760 | Hematopoietic prostaglandin D synthase | Homo sapiens | HOGENOM, OrthoDB, OrthoMCL BLAST | Thiazole, 4cid_379338Thiazolylamino, 12Pyrazolylthiazole, 13... |
| O35543 | Hematopoietic prostaglandin D synthase | HOGENOM, OrthoDB, OrthoMCL BLAST | CHEMBL4526974CHEMBL4473072CHEMBL4864805CHEMBL4873385... | |
| Q9JHF7 | Hematopoietic prostaglandin D synthase | Mus musculus | HOGENOM, OrthoDB, OrthoMCL BLAST | BW868CCAS_41598-07-6CAS_89617-02-7 |
| Q8MU52 | Glutathione S-transferase | Plasmodium falciparum | HOGENOM, OMA, OrthoDB, OrthoMCL, OrthoMCL BLAST | Coruleoellagic acid |
| P09210 | Glutathione S-transferase A2 | Homo sapiens | OrthoMCL BLAST | CHEMBL359933CHEMBL174893CID44286587 |
| P08263 | Glutathione S-transferase A1 | OrthoMCL BLAST | CHEMBL58507CHEMBL58135CHEMBL3310886CHEMBL3310887... |
Ortholog Group (OrthoMCL): OG6_100172
Most Similar Human Ortholog: O60760
TM-align score: 0.90 | RMSD: 1.80
Seq Identity: 0.30 | Length: 194 / 211
All Human Orthologs (OrthoMCL):
| Gene ID | Description |
|---|---|
| ENSG00000163106 | hematopoietic prostaglandin D synthase |
| ENSG00000182793 | glutathione S-transferase alpha 5 |
Protein Length: 211 | Molecular Weight (kDa): 24.789
UniProt ID(s): A0A144A1J6, Q8ILQ7, Q8MU52
PDB ID(s): None
Isoelectric Point: 6.26
Protein Domain Annotations:
| Source | Family ID | Description |
|---|---|---|
| InterPro | IPR004045 | Glutathione S-transferase, N-terminal |
| InterPro | IPR004046 | Glutathione S-transferase, C-terminal |
| InterPro | IPR036249 | Thioredoxin-like superfamily |
| InterPro | IPR036282 | Glutathione S-transferase, C-terminal domain superfamily |
| InterPro | IPR040079 | Glutathione Transferase family |
| PFam | PF02798 | Glutathione S-transferase, N-terminal |
| PFam | PF14497 | Glutathione S-transferase, C-terminal |
| Superfamily | SSF47616 | Glutathione S-transferase, C-terminal domain superfamily |
| Superfamily | SSF52833 | Thioredoxin-like superfamily |
MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:
Homozygous genotype calls only
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 1 | 7 | 7 | 12 |
| disruptive | 8 | 12 | 5 | 15 |
| missense | 8 | 12 | 5 | 14 |
Any inclusion in genotype call
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 3 | 19 | 3 | 11 |
| disruptive | 11 | 32 | 13 | 45 |
| missense | 11 | 24 | 10 | 29 |
PlasmoDB Total SNPs: 138
Non-coding: 129 | Synonymous: 3 | Nonsynonymous: 6 | Stop Codon: 0
| PMID | Title | Authors | DOI/Link |
|---|---|---|---|
| 12108547 | Glutathione S-transferase of the malarial parasite Plasmodium falciparum:characterization of a potential drug target. | Harwaldt P, Rahlfs S, Becker K | 10.1515/BC.2002.086 |
| 12368864 | Genome sequence of the human malaria parasite Plasmodium falciparum. | Gardner MJ, Hall N, ..., Barrell B | 10.1038/nature01097 |
| 15888443 | Cooperativity and pseudo-cooperativity in the glutathione S-transferase fromPlasmodium falciparum. | Liebau E, De Maria F, ..., Ricci G | 10.1074/jbc.M503889200 |
| 19531494 | Tetramerization and cooperativity in Plasmodium falciparum glutathioneS-transferase are mediated by atypic loop 113-119. | Liebau E, Dawood KF, ..., Ricci G | 10.1074/jbc.M109.015198 |
| 24792386 | Asn112 in Plasmodium falciparum glutathione S-transferase is essential forinduced reversible tetramerization by phosphate or pyrophosphate. | Quesada-Soriano I, Baron C, ..., Garcia-Fuentes L | 10.1016/j.bbapap.2014.04.017 |
| 32256353 | Structure-Based Screening of Plasmodium berghei Glutathione S-TransferaseIdentifies CB-27 as a Novel Antiplasmodial Compound. | Colon-Lorenzo EE, Colon-Lopez DD, ..., Serrano AE | 10.3389/fphar.2020.00246 |
| 38432355 | Exogenous monosodium glutamate exacerbates lipopolysaccharide-inducedneurobehavioral deficits, oxidative damage, neuroinflammation, and cholinergicdysfunction in rat brain | Asejeje FO, Abiola MA, ..., Ajayi AM | 10.1016/j.neulet.2024.137710 |