Product Description: glutathione reductase
SignalP Peptide: N/A
# Transmembrane Domains: 0
EC Numbers: 1.8.1.7 (Glutathione-disulfide reductase)
Curated GO (PlasmoDB):
| Type | GO Term | Name |
|---|---|---|
| Component | GO:0020011 | apicoplast |
| Component | GO:0005737 | cytoplasm |
| Function | GO:0004791 | thioredoxin-disulfide reductase activity |
| Process | GO:0045454 | cell redox homeostasis |
| Process | GO:0006979 | response to oxidative stress |
Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):
| Stage | LR class | MCA mean | MCA prop. zeros |
|---|---|---|---|
| Sporozoite | expressed | N/A | N/A |
| Ring | expressed | nd | nd |
| Trophozoite | expressed | nd | nd |
| Schizont | expressed | nd | nd |
| Gametocyte | expressed | nd | nd |
More info:
Old (Pf3D7v3) Gene ID: PF3D7_1419800.2
Resistome Missense Mutations: None
Resistome Compounds with Missense Mutations: None
Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)
Zhang Phenotype: Non - Mutable in CDS
MIS: 0.123 | MFS: -2.886 | #Insertions: 0
PlasmoGEM Phenotype: Dispensable (Pb ortholog: PBANKA_1023400)
RMgmDB ABS Phenotype: Different from wild type (Pb ortholog: PBANKA_1023400)
Modification: Disrupted | RMgm-2828
More info: PhenoPlasm Link
AlphaFill Uniprot ID: A0A144A3W0
"Best" AlphaFill ligand hit: FAD (flavin-adenine dinucleotide, Local RMSD=0.02) with 2EQ8 (Global RMSD=4.67)
BRENDA EC Inhibitors:
Orthology to BindingDB Entries:
| UniProt | Protein Name | Species | Homology Source | BindingDB Ligands |
|---|---|---|---|---|
| P00390 | Glutathione reductase, mitochondrial | HOGENOM, OMA, OrthoDB, OrthoMCL BLAST | KETOTIFENMeloxicam sodiumUS11028067, Example 2US11161815, Example 6... | |
| O15770 | Glutathione reductase | Plasmodium falciparum (isolate 3D7) | HOGENOM, OMA, OrthoDB, OrthoMCL BLAST | CHEMBL135287CHEMBL135287methylthioninium chloride |
| P41921 | Glutathione reductase | Saccharomyces cerevisiae | HOGENOM, OMA, OrthoDB, OrthoMCL, OrthoMCL BLAST | hypericum redPseudohypericin |
| A0A0H3JIZ9 | Glutathione-disulfide reductase | Escherichia coli | OMA | Sulfonamide derivative, 2 |
| P61076 | Thioredoxin reductase | Plasmodium falciparum (isolate 3D7) | OrthoDB | CHEMBL383084CHEMBL383084CHEMBL383084CHEMBL380953... |
| Q16881 | Thioredoxin reductase 1, cytoplasmic | OrthoDB | US9018255, 3aUS9018255, L-3aUS9018255, D-3aUS9018255, 3b... | |
| Q86VQ6 | Thioredoxin reductase 3 | OrthoDB | CHEMBL32856CHEMBL3814977CHEMBL89232CHEMBL89366... | |
| Q9Z0J5 | Thioredoxin reductase 2, mitochondrial | Rattus norvegicus | OrthoDB | PseudohypericinPseudohypericin |
| A4HSF7 | Trypanothione reductase | OrthoDB | CHEMBL242165CHEMBL4105040CHEMBL4080816CHEMBL4076896... | |
| Q962Y6 | Thioredoxin glutathione reductase | Schistosoma mansoni | OrthoDB | CHEMBL3322356CHEMBL3322293CHEMBL3322292CHEMBL3322288... |
| P28593 | Trypanothione reductase | Trypanosoma cruzi | OrthoMCL | CHEMBL276829CHEMBL279374CHEMBL17665CHEMBL160637... |
Ortholog Group (OrthoMCL): OG6_101659
Most Similar Human Ortholog: P00390
TM-align score: 0.87 | RMSD: 1.22
Seq Identity: 0.44 | Length: 444 / 500
All Human Orthologs (OrthoMCL):
| Gene ID | Description |
|---|---|
| ENSG00000104687 | glutathione-disulfide reductase |
Protein Length: 500 | Molecular Weight (kDa): 56.492
UniProt ID(s): A0A144A323, O15770
PDB ID(s): None
Isoelectric Point: 7.97
Protein Domain Annotations:
| Source | Family ID | Description |
|---|---|---|
| InterPro | IPR001100 | Pyridine nucleotide-disulphide oxidoreductase, class I |
| InterPro | IPR004099 | Pyridine nucleotide-disulphide oxidoreductase, dimerisation domain |
| InterPro | IPR012999 | Pyridine nucleotide-disulphide oxidoreductase, class I, active site |
| InterPro | IPR016156 | FAD/NAD-linked reductase, dimerisation domain superfamily |
| InterPro | IPR023753 | FAD/NAD(P)-binding domain |
| InterPro | IPR036188 | FAD/NAD(P)-binding domain superfamily |
| PFam | PF02852 | Pyridine nucleotide-disulphide oxidoreductase, dimerisation domain |
| PFam | PF07992 | FAD/NAD(P)-binding domain |
| Superfamily | SSF51905 | FAD/NAD(P)-binding domain superfamily |
| Superfamily | SSF55424 | FAD/NAD-linked reductase, dimerisation domain superfamily |
MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:
Homozygous genotype calls only
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 3 | 24 | 21 | 33 |
| disruptive | 21 | 36 | 20 | 36 |
| missense | 20 | 34 | 19 | 32 |
Any inclusion in genotype call
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 7 | 58 | 22 | 38 |
| disruptive | 36 | 66 | 44 | 104 |
| missense | 30 | 54 | 38 | 73 |
PlasmoDB Total SNPs: 122
Non-coding: 93 | Synonymous: 13 | Nonsynonymous: 16 | Stop Codon: 0
| PMID | Title | Authors | DOI/Link |
|---|---|---|---|
| 12368864 | Genome sequence of the human malaria parasite Plasmodium falciparum. | Gardner MJ, Hall N, ..., Barrell B | 10.1038/nature01097 |