Product Description: glycine--tRNA ligase
SignalP Peptide: MIIIVIFLLSMFFFENKTYC
# Transmembrane Domains: 0
EC Numbers: 6.1.1.14 (Glycine--tRNA ligase)
Curated GO (PlasmoDB):
| Type | GO Term | Name |
|---|---|---|
| Component | GO:0020011 | apicoplast |
| Component | GO:0005737 | cytoplasm |
| Component | GO:0005739 | mitochondrion |
| Function | GO:0004820 | glycine-tRNA ligase activity |
| Process | GO:0006426 | glycyl-tRNA aminoacylation |
| Process | GO:0070150 | mitochondrial glycyl-tRNA aminoacylation |
Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):
| Stage | LR class | MCA mean | MCA prop. zeros |
|---|---|---|---|
| Sporozoite | not expressed | N/A | N/A |
| Ring | expressed | 0.75 | 0.66 |
| Trophozoite | expressed | 1.03 | 0.48 |
| Schizont | expressed | 0.19 | 0.88 |
| Gametocyte | expressed | 0.42 | 0.77 |
More info:
Old (Pf3D7v3) Gene ID: PF3D7_1420400
Resistome Missense Mutations: None
Resistome Compounds with Missense Mutations: None
Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)
Zhang Phenotype: Non - Mutable in CDS
MIS: 0.12 | MFS: -2.951 | #Insertions: 0
PlasmoGEM Phenotype: Essential (Pb ortholog: PBANKA_1022800)
RMgmDB ABS Phenotype: N/A
More info: PhenoPlasm Link
AlphaFill Uniprot ID: Q8ILP6
"Best" AlphaFill ligand hit: AMP (adenosine monophosphate, Local RMSD=0.35) with 4KR2 (Global RMSD=2.31)
BRENDA EC Inhibitors:
| EC # | Name | EC Inhibitors |
|---|---|---|
| 6.1.1.14 | glycine-tRNA ligase | AMPKClcitratesperminephosphatenovobiocinOxolinic acidNalidixic acidAminoethyl-AMPInorganic sulfidep-chloromercuribenzoateglycyl sulfamoyl adenosine... |
Orthology to BindingDB Entries:
| UniProt | Protein Name | Species | Homology Source | BindingDB Ligands |
|---|---|---|---|---|
| Q04451 | Glycine--tRNA ligase | Bombyx mori | OrthoDB, OrthoMCL | AMPPCPGlycine sulfamoyl adenosine |
Ortholog Group (OrthoMCL): OG6_100673
Most Similar Human Ortholog: A0A6Q8PGW4
TM-align score: 0.86 | RMSD: 2.92
Seq Identity: 0.47 | Length: 566 / 889
All Human Orthologs (OrthoMCL):
| Gene ID | Description |
|---|---|
| ENSG00000106105 | glycyl-tRNA synthetase 1 |
Protein Length: 889 | Molecular Weight (kDa): 104.306
UniProt ID(s): Q8ILP6
PDB ID(s): None
Isoelectric Point: 7.99
Protein Domain Annotations:
| Source | Family ID | Description |
|---|---|---|
| InterPro | IPR002315 | Glycyl-tRNA synthetase |
| InterPro | IPR004154 | Anticodon-binding |
| InterPro | IPR027031 | Glycyl-tRNA synthetase/DNA polymerase subunit gamma-2 |
| InterPro | IPR033731 | Glycyl-tRNA synthetase-like core domain |
| PFam | PF03129 | Anticodon-binding |
| Superfamily | SSF52954 | N/A |
| Superfamily | SSF55681 |
MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:
Homozygous genotype calls only
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 5 | 43 | 22 | 54 |
| disruptive | 17 | 78 | 56 | 131 |
| missense | 9 | 67 | 53 | 106 |
Any inclusion in genotype call
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 13 | 90 | 27 | 68 |
| disruptive | 31 | 209 | 106 | 272 |
| missense | 16 | 169 | 89 | 173 |
PlasmoDB Total SNPs: 153
Non-coding: 91 | Synonymous: 33 | Nonsynonymous: 28 | Stop Codon: 1
| PMID | Title | Authors | DOI/Link |
|---|---|---|---|
| 12368864 | Genome sequence of the human malaria parasite Plasmodium falciparum. | Gardner MJ, Hall N, ..., Barrell B | 10.1038/nature01097 |
| 16267556 | A protein interaction network of the malaria parasite Plasmodium falciparum. | LaCount DJ, Vignali M, ..., Hughes RE | 10.1038/nature04104 |
| 22222968 | Dual targeting of aminoacyl-tRNA synthetases to the apicoplast and cytosol inPlasmodium falciparum. | Jackson KE, Pham JS, ..., Ralph SA | 10.1016/j.ijpara.2011.11.008 |
| 30212465 | Integrative proteomics and bioinformatic prediction enable a high-confidenceapicoplast proteome in malaria parasites. | Boucher MJ, Ghosh S, ..., Yeh E | 10.1371/journal.pbio.2005895 |