PF3D7_1420700 (P113)

Genome location: Pf3D7_14_v3:857,678..863,689(+)

Genome classification: Core

Function and Localization

Product Description: surface protein P113

SignalP Peptide: MKIPFFILHILLLQFLLCLIRC

# Transmembrane Domains: 1

EC Numbers: None

Curated GO (PlasmoDB):

Type	GO Term	Name
Component	GO:0009986	cell surface
Component	GO:0005886	plasma membrane
Component	GO:0020003	symbiont-containing vacuole
Component	GO:0020005	symbiont-containing vacuole membrane
Function	GO:0005515	protein binding
Process	GO:0007033	vacuole organization

Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):

Stage	LR class	MCA mean	MCA prop. zeros
Sporozoite	expressed	N/A	N/A
Ring	expressed	3.21	0.05
Trophozoite	expressed	2.34	0.13
Schizont	expressed	0.73	0.63
Gametocyte	possibly expressed	1.63	0.32

More info:

Resistome Mutations

Old (Pf3D7v3) Gene ID: PF3D7_1420700

Resistome Missense Mutations: None

Resistome Compounds with Missense Mutations: None

Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)

Essentiality (ABS)

Zhang Phenotype: Mutable in CDS

MIS: 0.991 | MFS: 0.436 | #Insertions: 1

PlasmoGEM Phenotype: N/A

RMgmDB ABS Phenotype: Not different from wild type (Pb ortholog: PBANKA_1022500)

Modification: Disrupted | RMgm-1001

More info: PhenoPlasm Link

Binding Evidence

AlphaFill Uniprot ID: Q8ILP3

"Best" AlphaFill ligand hit: No AlphaFill hits

No associated EC numbers

No evidence of orthology to BindingDB entries

Orthology Information

Ortholog Group (OrthoMCL): OG6_533097

No human ortholog(s)

Genetic Variation

MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:

Homozygous genotype calls only

variant type	common	rare	doubleton	singleton
synonymous	5	50	27	73
disruptive	22	98	62	157
missense	17	92	59	138

Any inclusion in genotype call

variant type	common	rare	doubleton	singleton
synonymous	13	97	40	75
disruptive	44	219	115	271
missense	33	194	97	180

PlasmoDB Total SNPs: 222

Non-coding: 153 | Synonymous: 37 | Nonsynonymous: 32 | Stop Codon: 0

Protein Information

Protein Length: 969 | Molecular Weight (kDa): 112.574

UniProt ID(s): C5HDX7, C5HDX9, C5HDY1, C5HDY3, C5HDY4, C5HDY6, C5HDY8, C5HDY9, C5HDZ1, C5HDZ2, C5HDZ3, Q8ILP3, Q9U0N8

PDB ID(s): 6Z2L

Isoelectric Point: 4.21

Protein Domain Annotations:

Source	Family ID	Description
InterPro	N/A	N/A
PFam	N/A	N/A
Superfamily	N/A	N/A

Associated Publications

PMID	Title	Authors	DOI/Link
12368864	Genome sequence of the human malaria parasite Plasmodium falciparum.	Gardner MJ, Hall N, ..., Barrell B	10.1038/nature01097
15477199	Myosin-like sequences in the malaria parasite Plasmodium falciparum bind humanerythrocyte membrane protein 4.1.	Lanzillotti R, Coetzer TL	https://pubmed.ncbi.nlm.nih.gov/15477199/
16267556	A protein interaction network of the malaria parasite Plasmodium falciparum.	LaCount DJ, Vignali M, ..., Hughes RE	10.1038/nature04104
24045696	Proteomic analysis of detergent-resistant membrane microdomains in trophozoiteblood stage of the human malaria parasite Plasmodium falciparum.	Yam XY, Birago C, ..., Ponzi M	10.1074/mcp.M113.029272
28186186	P113 is a merozoite surface protein that binds the N terminus of Plasmodiumfalciparum RH5.	Galaway F, Drought LG, ..., Wright GJ	10.1038/ncomms14333
32900802	The Structure of the Cysteine-Rich Domain of Plasmodium falciparum P113 Identifies the Location of the R H5 Binding Site	Campeotto I, Galaway F, ..., Wright GJ	10.1128/mBio.01566-20
35403274	The Plasmodium falciparum parasitophorous vacuole protein P113 interacts with theparasite protein export machinery and maintains normal vacuole architecture.	Bullen HE, Sanders PR, ..., Crabb BS	10.1111/mmi.14904
36753561	Anti-merozoite antibodies induce natural killer cell effector function and areassociated with immunity against malaria	Odera DO, Tuju J, ..., Williams TN	10.1126/scitranslmed.abn5993
34162366	The Plasmodium falciparum Rh5 invasion protein complex reveals an excess of rarevariant mutations	Ndwiga L, Osoti V, ..., Ochola-Oyier LI	10.1186/s12936-021-03815-x