PF3D7_1420900

Genome location: Pf3D7_14_v3:864,953..867,033(-)

Genome classification: Core

Function and Localization

Product Description: mannose-1-phosphate guanyltransferase, putative

SignalP Peptide: N/A

# Transmembrane Domains: 0

EC Numbers: 2.7.7.13 (Mannose-1-phosphate guanylyltransferase)

Curated GO (PlasmoDB):

Type	GO Term	Name
Component	GO:0005737	cytoplasm
Function	GO:0004475	mannose-1-phosphate guanylyltransferase activity
Process	GO:0009298	GDP-mannose biosynthetic process

Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):

Stage	LR class	MCA mean	MCA prop. zeros
Sporozoite	expressed	N/A	N/A
Ring	possibly expressed	0.05	0.97
Trophozoite	expressed	0.17	0.89
Schizont	expressed	0.18	0.87
Gametocyte	expressed	0.29	0.83

More info:

Resistome Mutations

Old (Pf3D7v3) Gene ID: PF3D7_1420900

Resistome Missense Mutations: None

Resistome Compounds with Missense Mutations: None

Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)

Essentiality (ABS)

Zhang Phenotype: Non - Mutable in CDS

MIS: 0.164 | MFS: -3.203 | #Insertions: 0

PlasmoGEM Phenotype: Slow (Pb ortholog: PBANKA_1022300)

Relative Growth Rate: 0.39 ± 0.12
Confidence: 5.57

RMgmDB ABS Phenotype: Different from wild type (Pb ortholog: PBANKA_1022300)

Modification: Disrupted | RMgm-2824

More info: PhenoPlasm Link

Binding Evidence

AlphaFill Uniprot ID: Q8ILP1

"Best" AlphaFill ligand hit: GDD (guanosine-5'-diphosphate-alpha-d-mannose, Local RMSD=0.82) with 7X8K (Global RMSD=4.08)

BRENDA EC Inhibitors:

EC #	Name	EC Inhibitors
2.7.7.13	mannose-1-phosphate guanylyltransferase	GDPGMPEDTAdiphosphateGDP-mannoseGDP-glucosemiltefosineammonium sulfateGDP-6-deoyxmannoseglucose 1-phosphatemannose 1-phosphate2-methyl-9H-pyrido[2,3-b]indole...

Orthology to BindingDB Entries:

UniProt	Protein Name	Species	Homology Source	BindingDB Ligands
Q9HU22	Glucose-1-phosphate thymidylyltransferase	Pseudomonas aeruginosa	OrthoMCL BLAST	Analogue of 1a, 1Analogue of 1a, 2Analogue of 1a, 5Analogue of 1a, 8a...
P43889	Bifunctional protein GlmU	Haemophilus influenzae	OrthoMCL BLAST	Sulfonamide, 1Sulfonamide, 2Sulfonamide, 3Sulfonamide, 4...
P0ACC7	Bifunctional protein GlmU	Escherichia coli	OrthoMCL BLAST	Sulfonamide, 1Sulfonamide, 3Sulfonamide, 4Sulfonamide, 5...

Orthology Information

Ortholog Group (OrthoMCL): OG6_100460

Most Similar Human Ortholog: Q9Y5P6

TM-align score: 0.97 | RMSD: 1.24

Seq Identity: 0.45 | Length: 357 / 408

All Human Orthologs (OrthoMCL):

Gene ID	Description
ENSG00000173540	GDP-mannose pyrophosphorylase B

Protein Information

Protein Length: 408 | Molecular Weight (kDa): 46.705

UniProt ID(s): Q8ILP1

PDB ID(s): None

Isoelectric Point: 6.58

Protein Domain Annotations:

Source	Family ID	Description
InterPro	IPR001451	Hexapeptide repeat
InterPro	IPR005835	Nucleotidyl transferase domain
InterPro	IPR011004	Trimeric LpxA-like superfamily
InterPro	IPR029044	Nucleotide-diphospho-sugar transferases
PFam	PF00132	Hexapeptide repeat
PFam	PF00483	Nucleotidyl transferase domain
Superfamily	SSF51161	Trimeric LpxA-like superfamily
Superfamily	SSF53448	Nucleotide-diphospho-sugar transferases

Genetic Variation

MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:

Homozygous genotype calls only

variant type	common	rare	doubleton	singleton
synonymous	3	19	12	19
disruptive	3	12	10	29
missense	3	12	10	27

Any inclusion in genotype call

variant type	common	rare	doubleton	singleton
synonymous	3	34	25	20
disruptive	5	38	26	73
missense	4	32	21	59

PlasmoDB Total SNPs: 135

Non-coding: 126 | Synonymous: 6 | Nonsynonymous: 3 | Stop Codon: 0

PMID	Title	Authors	DOI/Link
12368864	Genome sequence of the human malaria parasite Plasmodium falciparum.	Gardner MJ, Hall N, ..., Barrell B	10.1038/nature01097
34956931	Merozoite Proteins Discovered by qRT-PCR-Based Transcriptome Screening ofPlasmodium falciparum.	Hou N, Li S, ..., Chen Q	https://pubmed.ncbi.nlm.nih.gov/34956931/