Product Description: DNA-directed RNA polymerase III subunit RPC6, putative
SignalP Peptide: N/A
# Transmembrane Domains: 0
EC Numbers: 2.7.7.6 (DNA-directed RNA polymerase)
Curated GO (PlasmoDB):
| Type | GO Term | Name |
|---|---|---|
| Component | GO:0005666 | RNA polymerase III complex |
| Process | GO:0042797 | tRNA transcription by RNA polymerase III |
Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):
| Stage | LR class | MCA mean | MCA prop. zeros |
|---|---|---|---|
| Sporozoite | not expressed | N/A | N/A |
| Ring | expressed | 0.17 | 0.92 |
| Trophozoite | possibly expressed | 0.19 | 0.89 |
| Schizont | not expressed | 0.02 | 0.99 |
| Gametocyte | not expressed | 0.15 | 0.91 |
More info:
Old (Pf3D7v3) Gene ID: PF3D7_1421400
Resistome Missense Mutations: None
Resistome Compounds with Missense Mutations: None
Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)
Zhang Phenotype: Non - Mutable in CDS
MIS: 0.21 | MFS: -2.836 | #Insertions: 0
PlasmoGEM Phenotype: Essential (Pb ortholog: PBANKA_1021900)
RMgmDB ABS Phenotype: N/A
More info: PhenoPlasm Link
AlphaFill Uniprot ID: A0A144A2J0
"Best" AlphaFill ligand hit: No AlphaFill hits
BRENDA EC Inhibitors:
| EC # | Name | EC Inhibitors |
|---|---|---|
| 2.7.7.6 | DNA-directed RNA polymerase | BRENDA SOAP query unsuccessful |
No evidence of orthology to BindingDB entries
Ortholog Group (OrthoMCL): OG6_102251
Most Similar Human Ortholog: Q05DB8
TM-align score: 0.64 | RMSD: 3.63
Seq Identity: 0.23 | Length: 214 / 288
All Human Orthologs (OrthoMCL):
| Gene ID | Description |
|---|---|
| ENSG00000132664 | RNA polymerase III subunit F |
MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:
Homozygous genotype calls only
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 3 | 15 | 9 | 16 |
| disruptive | 2 | 13 | 7 | 26 |
| missense | 2 | 13 | 6 | 23 |
Any inclusion in genotype call
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 5 | 34 | 13 | 23 |
| disruptive | 4 | 43 | 20 | 71 |
| missense | 3 | 35 | 17 | 48 |
PlasmoDB Total SNPs: 138
Non-coding: 130 | Synonymous: 6 | Nonsynonymous: 2 | Stop Codon: 0
Protein Length: 288 | Molecular Weight (kDa): 34.138
UniProt ID(s): A0A144A2J0
PDB ID(s): None
Isoelectric Point: 8.66
Protein Domain Annotations:
| Source | Family ID | Description |
|---|---|---|
| InterPro | IPR007832 | RNA polymerase Rpc34 |
| InterPro | IPR016049 | RNA polymerase Rpc34-like |
| InterPro | IPR036390 | Winged helix DNA-binding domain superfamily |
| PFam | PF05158 | RNA polymerase Rpc34 |
| Superfamily | SSF46785 | Winged helix DNA-binding domain superfamily |
| PMID | Title | Authors | DOI/Link |
|---|---|---|---|
| 12368864 | Genome sequence of the human malaria parasite Plasmodium falciparum. | Gardner MJ, Hall N, ..., Barrell B | 10.1038/nature01097 |
| 10329159 | Mutagenesis of yeast TFIIIB70 reveals C-terminal residues critical forinteraction with TBP and C34 | Andrau JC, Sentenac A, Werner M | 10.1006/jmbi.1999.2724 |
| 7941739 | Organization of the centromeric region of chromosome XIV in Saccharomycescerevisiae | Lalo D, Stettler S, ..., Thuriaux P | 10.1002/yea.320100412 |
| 1400451 | An essential and specific subunit of RNA polymerase III (C) is encoded by geneRPC34 in Saccharomyces cerevisiae | Stettler S, Mariotte S, ..., Thuriaux P | https://pubmed.ncbi.nlm.nih.gov/1400451/ |
| 26423792 | Yeast Bud27 modulates the biogenesis of Rpc128 and Rpc160 subunits and theassembly of RNA polymerase III | Vernekar DV, Bhargava P | 10.1016/j.bbagrm.2015.09.010 |
| 7900425 | Twelve open reading frames revealed in the 23.6 kb segment flanking thecentromere on the Saccharomyces cerevisiae chromosome XIV right arm | Verhasselt P, Aert R, Voet M, Volckaert G | 10.1002/yea.320101013 |