About Gene List

PF3D7_1422500 (HRD1)

Genome location: Pf3D7_14_v3:906,564..909,327(+)

Genome classification: Core

Function and Localization

Product Description: ERAD-associated E3 ubiquitin-protein ligase HRD1

SignalP Peptide: N/A

# Transmembrane Domains: 5

EC Numbers: 2.3.2.27 (RING-type E3 ubiquitin transferase);6.3.2.19 (Transferred entry: 2.3.2.23, 2.3.2.27 and 6.2.1.45)

Curated GO (PlasmoDB):

Type GO Term Name
Component GO:0009986 cell surface
Component GO:0005783 endoplasmic reticulum
Component GO:0005789 endoplasmic reticulum membrane
Function GO:0061630 ubiquitin protein ligase activity
Function GO:0004842 ubiquitin-protein transferase activity
Process GO:0006511 ubiquitin-dependent protein catabolic process

Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):

Stage LR class MCA mean MCA prop. zeros
Sporozoite not expressed N/A N/A
Ring not expressed 0.08 0.96
Trophozoite expressed 0.48 0.71
Schizont expressed 0.61 0.65
Gametocyte expressed 0.98 0.52

More info:

Resistome Mutations

Old (Pf3D7v3) Gene ID: PF3D7_1422500

Resistome Missense Mutations: None

Resistome Compounds with Missense Mutations: None

Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)

Essentiality (ABS)

Zhang Phenotype: Non - Mutable in CDS

MIS: 0.121 | MFS: -3.176 | #Insertions: 0

PlasmoGEM Phenotype: N/A

RMgmDB ABS Phenotype: N/A

More info: PhenoPlasm Link

Binding Evidence

AlphaFill Uniprot ID: Q8ILM8

"Best" AlphaFill ligand hit: No AlphaFill hits

BRENDA EC Inhibitors:

EC # Name EC Inhibitors
2.3.2.27 RING-type E3 ubiquitin transferase EDTADTPAMLN4924RAPTA-CRAPTA-TRAPTA-EA1
6.3.2.19 ubiquitin-protein ligase No BRENDA inhibitors

No evidence of orthology to BindingDB entries

Orthology Information

Ortholog Group (OrthoMCL): OG6_102274

Most Similar Human Ortholog: E9PMA1

TM-align score: 0.88 | RMSD: 2.18

Seq Identity: 0.17 | Length: 230 / 510

All Human Orthologs (OrthoMCL):

Gene ID Description
ENSG00000162298 synoviolin 1

Genetic Variation

MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:

Homozygous genotype calls only

variant type common rare doubleton singleton
synonymous 6 30 12 30
disruptive 4 18 10 40
missense 4 18 10 37

Any inclusion in genotype call

variant type common rare doubleton singleton
synonymous 9 63 19 35
disruptive 8 58 36 115
missense 8 49 27 69

PlasmoDB Total SNPs: 157

Non-coding: 135 | Synonymous: 18 | Nonsynonymous: 4 | Stop Codon: 0

Protein Information

Protein Length: 510 | Molecular Weight (kDa): 59.786

UniProt ID(s): Q8ILM8

PDB ID(s): None

Isoelectric Point: 7.99

Protein Domain Annotations:

Source Family ID Description
InterPro IPR001841 Zinc finger, RING-type
PFam PF13639 Zinc finger, RING-type
Superfamily SSF57850 N/A

Associated Publications

PMID Title Authors DOI/Link
12368864 Genome sequence of the human malaria parasite Plasmodium falciparum. Gardner MJ, Hall N, ..., Barrell B 10.1038/nature01097
22912882 Characterization of the ubiquitylating components of the human malaria parasite'sprotein degradation pathway. Chung DW, Ponts N, ..., Le Roch KG 10.1371/journal.pone.0043477
34956931 Merozoite Proteins Discovered by qRT-PCR-Based Transcriptome Screening ofPlasmodium falciparum. Hou N, Li S, ..., Chen Q https://pubmed.ncbi.nlm.nih.gov/34956931/
37819693 Multiple quality control mechanisms monitor yeast chitin synthase folding in theendoplasmic reticulum Sanchez N, de Leon N, ..., Roncero C 10.1091/mbc.E23-05-0186