Product Description: actin-related protein ARP4
SignalP Peptide: N/A
# Transmembrane Domains: 0
EC Numbers: None
Curated GO (PlasmoDB):
| Type | GO Term | Name |
|---|---|---|
| Component | GO:0035267 | NuA4 histone acetyltransferase complex |
| Component | GO:0016514 | SWI/SNF complex |
| Component | GO:0005884 | actin filament |
| Component | GO:0005634 | nucleus |
| Function | GO:0003682 | chromatin binding |
| Function | GO:0005515 | protein binding |
| Function | GO:0005200 | structural constituent of cytoskeleton |
| Process | GO:0043044 | ATP-dependent chromatin remodeling |
| Process | GO:0006338 | chromatin remodeling |
| Process | GO:0007010 | cytoskeleton organization |
| Process | GO:0006897 | endocytosis |
| Process | GO:0006887 | exocytosis |
| Process | GO:0043967 | histone H4 acetylation |
| Process | GO:0010468 | regulation of gene expression |
| Process | GO:0006357 | regulation of transcription by RNA polymerase II |
| Process | GO:0020014 | schizogony |
Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):
| Stage | LR class | MCA mean | MCA prop. zeros |
|---|---|---|---|
| Sporozoite | not expressed | N/A | N/A |
| Ring | expressed | 0.28 | 0.86 |
| Trophozoite | expressed | 0.43 | 0.74 |
| Schizont | expressed | 0.06 | 0.96 |
| Gametocyte | possibly expressed | 0.37 | 0.78 |
More info:
Old (Pf3D7v3) Gene ID: PF3D7_1422800
Resistome Missense Mutations: V504D
Resistome Compounds with Missense Mutations: Proguanil
Resistome # Samples with Disruptive Mutations: 1 (1 missense, 1 "interesting" missense)
Zhang Phenotype: Non - Mutable in CDS
MIS: 0.155 | MFS: -2.815 | #Insertions: 0
PlasmoGEM Phenotype: Essential (Pb ortholog: PBANKA_1020600)
RMgmDB ABS Phenotype: N/A
More info: PhenoPlasm Link
AlphaFill Uniprot ID: Q8ILM5
"Best" AlphaFill ligand hit: SPD (n-(2-amino-propyl)-1,4-diaminobutane, Local RMSD=0.07) with 2HMP (Global RMSD=5.74)
No associated EC numbersOrthology to BindingDB Entries:
| UniProt | Protein Name | Species | Homology Source | BindingDB Ligands |
|---|---|---|---|---|
| P60010 | Actin [R335K] | HOGENOM | SCHEMBL1889389Latrunculin ASCHEMBL1889389Latrunculin A... |
Protein Length: 522 | Molecular Weight (kDa): 60.657
UniProt ID(s): Q8ILM5
PDB ID(s): None
Isoelectric Point: 5.36
Protein Domain Annotations:
| Source | Family ID | Description |
|---|---|---|
| InterPro | IPR004000 | Actin family |
| InterPro | IPR043129 | ATPase, nucleotide binding domain |
| PFam | PF00022 | Actin family |
| Superfamily | SSF53067 | ATPase, nucleotide binding domain |
MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:
Homozygous genotype calls only
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 7 | 19 | 22 | 34 |
| disruptive | 12 | 32 | 19 | 69 |
| missense | 12 | 29 | 19 | 62 |
Any inclusion in genotype call
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 9 | 54 | 26 | 36 |
| disruptive | 16 | 82 | 48 | 126 |
| missense | 14 | 76 | 42 | 87 |
PlasmoDB Total SNPs: 107
Non-coding: 56 | Synonymous: 38 | Nonsynonymous: 13 | Stop Codon: 0
| PMID | Title | Authors | DOI/Link |
|---|---|---|---|
| 12368864 | Genome sequence of the human malaria parasite Plasmodium falciparum. | Gardner MJ, Hall N, ..., Barrell B | 10.1038/nature01097 |
| 23181666 | Organellar proteomics reveals hundreds of novel nuclear proteins in the malariaparasite Plasmodium falciparum. | Oehring SC, Woodcroft BJ, ..., Voss TS | 10.1186/gb-2012-13-11-r108 |
| 32552779 | Actin-related protein Arp4 regulates euchromatic gene expression and developmentthrough H2A.Z deposition in blood-stage Plasmodium falciparum. | Liu H, Cui XY, ..., Xia H | 10.1186/s13071-020-04139-6 |
| 29559617 | Architecture of the Saccharomyces cerevisiae NuA4/TIP60 complex | Wang X, Ahmad S, ..., Cai G | 10.1038/s41467-018-03504-5 |
| 36983497 | Yeast Chromatin Mutants Reveal Altered mtDNA Copy Number and ImpairedMitochondrial Membrane Potential | Staneva D, Vasileva B, ..., Georgieva M | 10.3390/jof9030329 |
| 27457955 | Crystal structure of a nuclear actin ternary complex | Cao T, Sun L, ..., Chen Z | 10.1073/pnas.1602818113 |
| 36263929 | Structure and flexibility of the yeast NuA4 histone acetyltransferase complex | Zukin SA, Marunde MR, ..., Patel AB | 10.7554/eLife.81400 |
| 34752857 | Actin-related protein 4: An unconventional negative regulator of mitochondrial calcium in protozoan parasite Leishmania | Prasadareddy Kajuluri L, Singh A, ..., Sahasrabuddhe AA | 10.1016/j.mito.2021.11.001 |
| 36078161 | Actin-Related Protein 4 and Linker Histone Sustain Yeast Replicative Ageing | Molon M, Stepien K, ..., Georgieva M | 10.3390/cells11172754 |
| 28270832 | Actin Family Proteins in the Human INO80 Chromatin Remodeling Complex ExhibitFunctional Roles in the Induction of Heme Oxygenase-1 with Hemin | Takahashi Y, Murakami H, ..., Harata M | 10.3389/fgene.2017.00017 |
| 26298820 | Actin, actin-related proteins and profilin in diatoms: a comparative genomicanalysis | Aumeier C, Polinski E, Menzel D | 10.1016/j.margen.2015.07.002 |