Product Description: nucleolar GTP-binding protein 2, putative
SignalP Peptide: N/A
# Transmembrane Domains: 0
EC Numbers: 3.6.5.1 (Heterotrimeric G-protein GTPase)
Curated GO (PlasmoDB):
| Type | GO Term | Name |
|---|---|---|
| Component | GO:0005730 | nucleolus |
| Component | GO:0030687 | preribosome, large subunit precursor |
| Function | GO:0003924 | GTPase activity |
| Process | GO:0000055 | ribosomal large subunit export from nucleus |
Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):
| Stage | LR class | MCA mean | MCA prop. zeros |
|---|---|---|---|
| Sporozoite | not expressed | N/A | N/A |
| Ring | expressed | 1.09 | 0.53 |
| Trophozoite | expressed | 1.10 | 0.46 |
| Schizont | expressed | 0.35 | 0.79 |
| Gametocyte | not expressed | 0.68 | 0.63 |
More info:
Old (Pf3D7v3) Gene ID: PF3D7_1423000
Resistome Missense Mutations: None
Resistome Compounds with Missense Mutations: None
Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)
Zhang Phenotype: Non - Mutable in CDS
MIS: 0.133 | MFS: -3.163 | #Insertions: 0
PlasmoGEM Phenotype: Essential (Pb ortholog: PBANKA_1020400)
RMgmDB ABS Phenotype: N/A
More info: PhenoPlasm Link
AlphaFill Uniprot ID: Q8ILM2
"Best" AlphaFill ligand hit: GDP (guanosine-5'-diphosphate, Local RMSD=1.37) with 6G0Z (Global RMSD=5.42)
BRENDA EC Inhibitors:
| EC # | Name | EC Inhibitors |
|---|---|---|
| 3.6.5.1 | heterotrimeric G-protein GTPase | GDPGpp(NH)pYM-254890phosducinpertussis toxinPasteurella multocida toxinLeu-Gly-Asn repeat-enriched proteinregulator of G protein signaling proteinbetagamma subunit of the hetreotrimeric G protein |
No evidence of orthology to BindingDB entries
Ortholog Group (OrthoMCL): OG6_101829
Most Similar Human Ortholog: Q13823
TM-align score: 0.84 | RMSD: 2.36
Seq Identity: 0.42 | Length: 432 / 487
All Human Orthologs (OrthoMCL):
| Gene ID | Description |
|---|---|
| ENSG00000134697 | G protein nucleolar 2 |
MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:
Homozygous genotype calls only
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 1 | 19 | 15 | 31 |
| disruptive | 3 | 26 | 21 | 63 |
| missense | 3 | 24 | 20 | 55 |
Any inclusion in genotype call
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 4 | 42 | 24 | 33 |
| disruptive | 14 | 83 | 43 | 108 |
| missense | 7 | 62 | 36 | 80 |
PlasmoDB Total SNPs: 113
Non-coding: 91 | Synonymous: 16 | Nonsynonymous: 6 | Stop Codon: 0
Protein Length: 487 | Molecular Weight (kDa): 57.172
UniProt ID(s): Q8ILM2
PDB ID(s): None
Isoelectric Point: 10.37
Protein Domain Annotations:
| Source | Family ID | Description |
|---|---|---|
| InterPro | IPR006073 | GTP binding domain |
| InterPro | IPR012971 | Nucleolar GTP-binding protein 2, N-terminal domain |
| InterPro | IPR024929 | Nucleolar GTP-binding protein 2 |
| InterPro | IPR027417 | P-loop containing nucleoside triphosphate hydrolase |
| PFam | PF01926 | GTP binding domain |
| PFam | PF08153 | Nucleolar GTP-binding protein 2, N-terminal domain |
| Superfamily | SSF52540 | P-loop containing nucleoside triphosphate hydrolase |
| PMID | Title | Authors | DOI/Link |
|---|---|---|---|
| 12368864 | Genome sequence of the human malaria parasite Plasmodium falciparum. | Gardner MJ, Hall N, ..., Barrell B | 10.1038/nature01097 |
| 34125911 | The ribosome assembly factor Nop53 has a structural role in the formation of nuclear pre-60S intermediates, affecting late maturation events | Bagatelli FFM, de Luna Vitorino FN, da Cunha JPC, Oliveira CC | 10.1093/nar/gkab494 |
| 35615984 | Translation initiation factor eIF4G1 modulates assembly of the polypeptide exittunnel region in yeast ribosome biogenesis | Tseng YT, Sung YC, Liu CY, Lo KY | 10.1242/jcs.259540 |
| 37811893 | Yeast ribosome biogenesis factors Puf6 and Nog2 and ribosomal proteins uL2 andeL43 act in concert to facilitate the release of nascent large ribosomal subunitsfrom the nucleolus | LaPeruta AJ, Hedayati S, ..., Woolford JL | 10.1093/nar/gkad794 |