PF3D7_1423100

Genome location: Pf3D7_14_v3:927,631..931,876(+)

Genome classification: Core

Function and Localization

Product Description: ankyrin-repeat protein, putative

SignalP Peptide: N/A

# Transmembrane Domains: 0

EC Numbers: None

Curated GO (PlasmoDB): None

Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):

Stage	LR class	MCA mean	MCA prop. zeros
Sporozoite	expressed	N/A	N/A
Ring	not expressed	0.03	0.99
Trophozoite	possibly expressed	0.03	0.98
Schizont	expressed	0.49	0.73
Gametocyte	expressed	0.36	0.79

More info:

Resistome Mutations

Old (Pf3D7v3) Gene ID: PF3D7_1423100

Resistome Missense Mutations: V148I

Resistome Compounds with Missense Mutations: MMV665794

Resistome # Samples with Disruptive Mutations: 1 (1 missense, 0 "interesting" missense)

Essentiality (ABS)

Zhang Phenotype: Mutable in CDS

MIS: 1 | MFS: -0.061 | #Insertions: 6

PlasmoGEM Phenotype: Dispensable (Pb ortholog: PBANKA_1020300)

Relative Growth Rate: 0.95 ± 0.12
Confidence: 5.61

RMgmDB ABS Phenotype: Different from wild type (Pb ortholog: PBANKA_1020300)

Modification: Disrupted | RMgm-2815

More info: PhenoPlasm Link

Binding Evidence

AlphaFill Uniprot ID: Q8ILM1

"Best" AlphaFill ligand hit: TAR (d(-)-tartaric acid, Local RMSD=0.11) with 6MOJ (Global RMSD=6.41)

No associated EC numbers

No evidence of orthology to BindingDB entries

Orthology Information

Ortholog Group (OrthoMCL): OG6_100012

Most Similar Human Ortholog: C9JY51

TM-align score: 0.93 | RMSD: 1.05

Seq Identity: 0.30 | Length: 76 / 592

All Human Orthologs (OrthoMCL):

Gene ID	Description
ENSG00000029534	ankyrin 1
ENSG00000065413	ankyrin repeat domain 44
ENSG00000139645	ankyrin repeat domain 52
ENSG00000145362	ankyrin 2
ENSG00000151150	ankyrin 3
ENSG00000151458	ankyrin repeat domain 50
ENSG00000206560	ankyrin repeat domain 28

Genetic Variation

MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:

Homozygous genotype calls only

variant type	common	rare	doubleton	singleton
synonymous	3	31	15	36
disruptive	4	41	21	93
missense	3	37	17	74

Any inclusion in genotype call

variant type	common	rare	doubleton	singleton
synonymous	7	53	30	34
disruptive	20	86	76	148
missense	8	71	60	98

PlasmoDB Total SNPs: 218

Non-coding: 182 | Synonymous: 27 | Nonsynonymous: 9 | Stop Codon: 0

Protein Information

Protein Length: 592 | Molecular Weight (kDa): 69.56

UniProt ID(s): Q8ILM1

PDB ID(s): None

Isoelectric Point: 10

Protein Domain Annotations:

Source	Family ID	Description
InterPro	IPR002110	Ankyrin repeat
InterPro	IPR020683	Ankyrin repeat-containing domain
InterPro	IPR036770	Ankyrin repeat-containing domain superfamily
PFam	PF12796	Ankyrin repeat-containing domain
PFam	PF13637
Superfamily	SSF48403	Ankyrin repeat-containing domain superfamily