Product Description: serine/threonine protein phosphatase 7
SignalP Peptide: N/A
# Transmembrane Domains: 0
EC Numbers: 3.1.3.16 (Protein-serine/threonine phosphatase)
Curated GO (PlasmoDB):
| Type | GO Term | Name |
|---|---|---|
| Component | GO:0005737 | cytoplasm |
| Component | GO:0005829 | cytosol |
| Component | GO:0005634 | nucleus |
| Function | GO:0004722 | protein serine/threonine phosphatase activity |
| Process | GO:0006470 | protein dephosphorylation |
Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):
| Stage | LR class | MCA mean | MCA prop. zeros |
|---|---|---|---|
| Sporozoite | not expressed | N/A | N/A |
| Ring | not expressed | 0.23 | 0.90 |
| Trophozoite | not expressed | 0.07 | 0.96 |
| Schizont | expressed | 1.77 | 0.39 |
| Gametocyte | not expressed | 0.36 | 0.80 |
More info:
Old (Pf3D7v3) Gene ID: PF3D7_1423300
Resistome Missense Mutations: None
Resistome Compounds with Missense Mutations: None
Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)
Zhang Phenotype: Non - Mutable in CDS
MIS: 0.357 | MFS: -2.633 | #Insertions: 0
PlasmoGEM Phenotype: Essential (Pb ortholog: PBANKA_1020100)
RMgmDB ABS Phenotype: Different from wild type (Pb ortholog: PBANKA_1020100)
Modification: Tagged | RMgm-1071
More info: PhenoPlasm Link
AlphaFill Uniprot ID: Q8ILL9
"Best" AlphaFill ligand hit: NHE (2-[n-cyclohexylamino]ethane sulfonic acid, Local RMSD=0.30) with 6GHM (Global RMSD=3.37)
BRENDA EC Inhibitors:
| EC # | Name | EC Inhibitors |
|---|---|---|
| 3.1.3.16 | protein-serine/threonine phosphatase | ATPADPUDPAMPGTPUTPUMPNaFEDTAEGTAcAMPNADPH... |
No evidence of orthology to BindingDB entries
MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:
Homozygous genotype calls only
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 5 | 33 | 30 | 63 |
| disruptive | 5 | 33 | 20 | 68 |
| missense | 5 | 33 | 19 | 62 |
Any inclusion in genotype call
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 10 | 88 | 46 | 65 |
| disruptive | 11 | 100 | 69 | 173 |
| missense | 8 | 84 | 58 | 117 |
PlasmoDB Total SNPs: 432
Non-coding: 385 | Synonymous: 42 | Nonsynonymous: 4 | Stop Codon: 1
Protein Length: 959 | Molecular Weight (kDa): 112.585
PDB ID(s): None
Isoelectric Point: 6.94
Protein Domain Annotations:
| Source | Family ID | Description |
|---|---|---|
| InterPro | IPR000048 | IQ motif, EF-hand binding site |
| InterPro | IPR002048 | EF-hand domain |
| InterPro | IPR004843 | Calcineurin-like phosphoesterase domain, ApaH type |
| InterPro | IPR006186 | Serine/threonine-specific protein phosphatase/bis(5-nucleosyl)-tetraphosphatase |
| InterPro | IPR011992 | EF-hand domain pair |
| PFam | PF00149 | Calcineurin-like phosphoesterase domain, ApaH type |
| PFam | PF13499 | EF-hand domain |
| Superfamily | SSF47473 | EF-hand domain pair |
| Superfamily | SSF56300 |
| PMID | Title | Authors | DOI/Link |
|---|---|---|---|
| 11377737 | Characterization of a novel serine/threonine protein phosphatase (PfPPJ) from themalaria parasite, Plasmodium falciparum. | Dobson S, Bracchi V, Chakrabarti D, Barik S | 10.1016/s0166-6851(01)00260-2 |
| 12368864 | Genome sequence of the human malaria parasite Plasmodium falciparum. | Gardner MJ, Hall N, ..., Barrell B | 10.1038/nature01097 |
| 16629662 | Regulation of protein phosphatase type 1 and cell cycle progression by PfLRR1, anovel leucine-rich repeat protein of the human malaria parasite Plasmodiumfalciparum. | Daher W, Browaeys E, ..., Khalife J | 10.1111/j.1365-2958.2006.05119.x |
| 23181666 | Organellar proteomics reveals hundreds of novel nuclear proteins in the malariaparasite Plasmodium falciparum. | Oehring SC, Woodcroft BJ, ..., Voss TS | 10.1186/gb-2012-13-11-r108 |
| 25011111 | Genome-wide functional analysis of Plasmodium protein phosphatases reveals keyregulators of parasite development and differentiation. | Guttery DS, Poulin B, ..., Tewari R | 10.1016/j.chom.2014.05.020 |
| 38026848 | Quantitative and modularized CRISPR/dCas9-dCpf1 dual function system in Saccharomyces cerevisiae | Feng Q, Ning X, ..., Li C | 10.3389/fbioe.2023.1218832 |