About Gene List

PF3D7_1423800 (VPS3)

Genome location: Pf3D7_14_v3:967,040..972,619(+)

Genome classification: Core

Function and Localization

Product Description: vacuolar protein sorting-associated protein 3, putative

SignalP Peptide: N/A

# Transmembrane Domains: 0

EC Numbers: None

Curated GO (PlasmoDB):

Type GO Term Name
Component GO:0005737 cytoplasm
Component GO:0016020 membrane
Process GO:0006914 autophagy
Process GO:0034058 endosomal vesicle fusion

Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):

Stage LR class MCA mean MCA prop. zeros
Sporozoite expressed N/A N/A
Ring expressed 0.74 0.66
Trophozoite expressed 0.74 0.59
Schizont expressed 0.28 0.82
Gametocyte expressed 0.58 0.68

More info:

Resistome Mutations

Old (Pf3D7v3) Gene ID: PF3D7_1423800

Resistome Missense Mutations: D780E

Resistome Compounds with Missense Mutations: AN13762

Resistome # Samples with Disruptive Mutations: 1 (1 missense, 0 "interesting" missense)

Essentiality (ABS)

Zhang Phenotype: Mutable in CDS

MIS: 0.77 | MFS: -3.028 | #Insertions: 1

PlasmoGEM Phenotype: N/A

RMgmDB ABS Phenotype: N/A

More info: PhenoPlasm Link

Binding Evidence

AlphaFill Uniprot ID: Q8ILL4

"Best" AlphaFill ligand hit: No AlphaFill hits

No associated EC numbers

No evidence of orthology to BindingDB entries

Orthology Information

Ortholog Group (OrthoMCL): OG6_532347

No human ortholog(s)

Genetic Variation

MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:

Homozygous genotype calls only

variant type common rare doubleton singleton
synonymous 14 59 41 71
disruptive 46 188 95 261
missense 25 156 81 205

Any inclusion in genotype call

variant type common rare doubleton singleton
synonymous 23 132 71 95
disruptive 98 436 187 430
missense 53 318 142 276

PlasmoDB Total SNPs: 289

Non-coding: 134 | Synonymous: 102 | Nonsynonymous: 51 | Stop Codon: 2

Protein Information

Protein Length: 1468 | Molecular Weight (kDa): 176.735

UniProt ID(s): Q8ILL4

PDB ID(s): None

Isoelectric Point: 8.68

Protein Domain Annotations:

Source Family ID Description
InterPro IPR019453 Vacuolar sorting protein 39/Transforming growth factor beta receptor-associated domain 2
InterPro IPR032914 Vam6/VPS39/TRAP1 family
InterPro IPR036322 WD40-repeat-containing domain superfamily
PFam PF10367 Vacuolar sorting protein 39/Transforming growth factor beta receptor-associated domain 2
Superfamily SSF50978 WD40-repeat-containing domain superfamily

Associated Publications

PMID Title Authors DOI/Link
12368864 Genome sequence of the human malaria parasite Plasmodium falciparum. Gardner MJ, Hall N, ..., Barrell B 10.1038/nature01097
16267556 A protein interaction network of the malaria parasite Plasmodium falciparum. LaCount DJ, Vignali M, ..., Hughes RE 10.1038/nature04104
21777356 An overexpression screen in Saccharomyces cerevisiae identifies novel genes thataffect endocytic protein trafficking Arlt H, Perz A, Ungermann C 10.1111/j.1600-0854.2011.01252.x