PF3D7_1425400

Genome location: Pf3D7_14_v3:988,897..992,990(+)

Genome classification: Core

Function and Localization

Product Description: DEAD/DEAH box helicase, putative

SignalP Peptide: N/A

# Transmembrane Domains: 0

EC Numbers: 3.6.4.13 (RNA helicase)

Curated GO (PlasmoDB):

Type	GO Term	Name
Component	GO:0071013	catalytic step 2 spliceosome
Function	GO:0003723	RNA binding
Function	GO:0004386	helicase activity
Process	GO:0000398	mRNA splicing, via spliceosome

Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):

Stage	LR class	MCA mean	MCA prop. zeros
Sporozoite	possibly expressed	N/A	N/A
Ring	possibly expressed	0.14	0.93
Trophozoite	expressed	0.28	0.83
Schizont	expressed	1.08	0.52
Gametocyte	expressed	0.94	0.52

More info:

Resistome Mutations

Old (Pf3D7v3) Gene ID: PF3D7_1425400

Resistome Missense Mutations: None

Resistome Compounds with Missense Mutations: None

Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)

Essentiality (ABS)

Zhang Phenotype: Mutable in CDS

MIS: 1 | MFS: -0.212 | #Insertions: 6

PlasmoGEM Phenotype: Dispensable (Pb ortholog: PBANKA_1019100)

Relative Growth Rate: 1.00 ± 0.11
Confidence: 5.76

RMgmDB ABS Phenotype: Different from wild type (Pb ortholog: PBANKA_1019100)

Modification: Disrupted | RMgm-2811

More info: PhenoPlasm Link

Binding Evidence

AlphaFill Uniprot ID: Q8ILK9

"Best" AlphaFill ligand hit: AGS (phosphothiophosphoric acid-adenylate ester, Local RMSD=0.20) with 6QV4 (Global RMSD=8.24)

BRENDA EC Inhibitors:

EC #	Name	EC Inhibitors
3.6.4.13	RNA helicase	ATPADPUDPAMPGTPGDPUTPKClNEMCDPITPTDP...

Orthology to BindingDB Entries:

UniProt	Protein Name	Species	Homology Source	BindingDB Ligands
O75417	DNA polymerase theta		OrthoDB	CHEMBL5185248CHEMBL5190448CHEMBL5175626CHEMBL5188740...

Orthology Information

Ortholog Group (OrthoMCL): OG6_533271

No human ortholog(s)

Protein Information

Protein Length: 1236 | Molecular Weight (kDa): 146.282

UniProt ID(s): Q8ILK9

PDB ID(s): None

Isoelectric Point: 8.8

Protein Domain Annotations:

Source	Family ID	Description
InterPro	IPR001650	Helicase, C-terminal
InterPro	IPR011545	DEAD/DEAH box helicase domain
InterPro	IPR014001	Helicase superfamily 1/2, ATP-binding domain
InterPro	IPR027417	P-loop containing nucleoside triphosphate hydrolase
PFam	PF00270	DEAD/DEAH box helicase domain
PFam	PF00271	Helicase, C-terminal
Superfamily	SSF52540	P-loop containing nucleoside triphosphate hydrolase

Genetic Variation

MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:

Homozygous genotype calls only

variant type	common	rare	doubleton	singleton
synonymous	6	54	29	70
disruptive	43	154	85	192
missense	35	133	77	172

Any inclusion in genotype call

variant type	common	rare	doubleton	singleton
synonymous	18	96	52	82
disruptive	82	314	148	321
missense	59	262	125	231

PlasmoDB Total SNPs: 132

Non-coding: 42 | Synonymous: 54 | Nonsynonymous: 35 | Stop Codon: 1