Genome location: Pf3D7_14_v3:1,020,917..1,023,165(-)
Genome classification: Core
Product Description: protein arginine N-methyltransferase 1
SignalP Peptide: N/A
# Transmembrane Domains: 0
EC Numbers: 2.1.1.125 (Transferred entry: 2.1.1.319, 2.1.1.320, 2.1.1.321 and 2.1.1.322);2.1.1.319 (Type I protein arginine methyltransferase)
Curated GO (PlasmoDB):
| Type | GO Term | Name |
|---|---|---|
| Component | GO:0005737 | cytoplasm |
| Component | GO:0005634 | nucleus |
| Function | GO:0016274 | protein-arginine N-methyltransferase activity |
| Process | GO:0016070 | RNA metabolic process |
| Process | GO:0006396 | RNA processing |
| Process | GO:0016571 | histone methylation |
| Process | GO:0042254 | ribosome biogenesis |
Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):
| Stage | LR class | MCA mean | MCA prop. zeros |
|---|---|---|---|
| Sporozoite | possibly expressed | N/A | N/A |
| Ring | expressed | 0.44 | 0.80 |
| Trophozoite | expressed | 0.96 | 0.51 |
| Schizont | expressed | 0.17 | 0.89 |
| Gametocyte | expressed | 0.40 | 0.78 |
More info:
Old (Pf3D7v3) Gene ID: PF3D7_1426200
Resistome Missense Mutations: None
Resistome Compounds with Missense Mutations: None
Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)
Zhang Phenotype: Mutable in CDS
MIS: 0.967 | MFS: 0.148 | #Insertions: 1
PlasmoGEM Phenotype: Slow (Pb ortholog: PBANKA_1018400)
RMgmDB ABS Phenotype: Different from wild type (Pb ortholog: PBANKA_1018400)
Modification: Disrupted | RMgm-2808
More info: PhenoPlasm Link
AlphaFill Uniprot ID: Q8ILK1
"Best" AlphaFill ligand hit: DXE (1,2-dimethoxyethane, Local RMSD=0.09) with 5K8W (Global RMSD=5.53)
BRENDA EC Inhibitors:
| EC # | Name | EC Inhibitors |
|---|---|---|
| 2.1.1.125 | histone-arginine N-methyltransferase | BRENDA SOAP query unsuccessful |
| 2.1.1.319 | type I protein arginine methyltransferase | TC-E5003sinefunginBay 11-7082Bay 11-7085phenyl vinyl sulfoneS-adenosylhomocysteinephenyl vinyl sulfonateS-adenosyl-L-homocysteineN-(3-bromobenzyl)-1-(2-(methylamino)ethyl)piperidin-4-amineN2-[6-(4-carbamimidoylanilino)-5-nitropyrimidin-4-yl]glycinamide4-[(6-amino-5-nitropyrimidin-4-yl)amino]benzene-1-carboximidamide4-[(6-anilino-5-nitropyrimidin-4-yl)amino]benzene-1-carboximidamide... |
Orthology to BindingDB Entries:
| UniProt | Protein Name | Species | Homology Source | BindingDB Ligands |
|---|---|---|---|---|
| Q9NR22 | Protein arginine N-methyltransferase 8 | OMA, OrthoDB | N1-Methyl-N1-((5-(3-(trifluoromethyl)phenyl)-2H-1,2,3-triazol-4-yl)methyl)ethane-1,2-diamine (Compound 1)N1-Methyl-N1-((4-(3-(trifluoromethyl)phenyl)-1H-pyrrol-3-yl)methyl)ethane-1,2-diamine (Compound 2)MS023 (Compound 3)CHEMBL3409550... | |
| Q96LA8 | Protein arginine N-methyltransferase 6 | OrthoDB | N1-Methyl-N1-((5-(3-(trifluoromethyl)phenyl)-2H-1,2,3-triazol-4-yl)methyl)ethane-1,2-diamine (Compound 1)N1-Methyl-N1-((4-(3-(trifluoromethyl)phenyl)-1H-pyrrol-3-yl)methyl)ethane-1,2-diamine (Compound 2)MS023 (Compound 3)US11028083, Example 2... | |
| Q63009 | Protein arginine N-methyltransferase 1 | Rattus norvegicus | OrthoDB | CHEMBL4084293CHEMBL4094513CHEMBL4073964CHEMBL4097780... |
| Q99873 | Protein arginine N-methyltransferase 1 | OrthoDB | N1-Methyl-N1-((4-(3-(trifluoromethyl)phenyl)-1H-pyrrol-3-yl)methyl)ethane-1,2-diamine (Compound 2)MS023 (Compound 3)US11028083, Example 94aUS11028083, Example 94b... | |
| O60678 | Protein arginine N-methyltransferase 3 | OrthoDB | N1-Methyl-N1-((4-(3-(trifluoromethyl)phenyl)-1H-pyrrol-3-yl)methyl)ethane-1,2-diamine (Compound 2)MS023 (Compound 3)NSC-47762CHEMBL2325441... | |
| Q9WVG6 | Histone-arginine methyltransferase CARM1 | Mus musculus | OrthoDB | pyrazole scaffold, 1bpyrazole scaffold, 7apyrazole scaffold, 10apyrazole scaffold, 13... |
| Q86X55 | Histone-arginine methyltransferase CARM1 | OrthoDB | N1-Methyl-N1-((4-(3-(trifluoromethyl)phenyl)-1H-pyrrol-3-yl)methyl)ethane-1,2-diamine (Compound 2)MS023 (Compound 3)US10633389, Example 1-1US10633389, Example 2-1... |
Ortholog Group (OrthoMCL): OG6_101444
Most Similar Human Ortholog: E9PKG1
TM-align score: 0.99 | RMSD: 0.81
Seq Identity: 0.47 | Length: 325 / 401
All Human Orthologs (OrthoMCL):
| Gene ID | Description |
|---|---|
| ENSG00000111218 | protein arginine methyltransferase 8 |
| ENSG00000126457 | protein arginine methyltransferase 1 |
Protein Length: 401 | Molecular Weight (kDa): 47.446
UniProt ID(s): Q8ILK1
PDB ID(s): None
Isoelectric Point: 6.5
Protein Domain Annotations:
| Source | Family ID | Description |
|---|---|---|
| InterPro | IPR029063 | S-adenosyl-L-methionine-dependent methyltransferase |
| InterPro | IPR041698 | Methyltransferase domain 25 |
| PFam | PF13649 | Methyltransferase domain 25 |
| Superfamily | SSF53335 | S-adenosyl-L-methionine-dependent methyltransferase |
MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:
Homozygous genotype calls only
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 0 | 19 | 13 | 26 |
| disruptive | 0 | 9 | 5 | 15 |
| missense | 0 | 9 | 4 | 13 |
Any inclusion in genotype call
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 2 | 40 | 10 | 24 |
| disruptive | 2 | 18 | 12 | 29 |
| missense | 0 | 15 | 11 | 22 |
PlasmoDB Total SNPs: 125
Non-coding: 106 | Synonymous: 19 | Nonsynonymous: 0 | Stop Codon: 0
| PMID | Title | Authors | DOI/Link |
|---|---|---|---|
| 12368864 | Genome sequence of the human malaria parasite Plasmodium falciparum. | Gardner MJ, Hall N, ..., Barrell B | 10.1038/nature01097 |
| 19344311 | Characterization of PRMT1 from Plasmodium falciparum. | Fan Q, Miao J, Cui L, Cui L | 10.1042/BJ20090185 |
| 23181666 | Organellar proteomics reveals hundreds of novel nuclear proteins in the malariaparasite Plasmodium falciparum. | Oehring SC, Woodcroft BJ, ..., Voss TS | 10.1186/gb-2012-13-11-r108 |