Genome location: Pf3D7_14_v3:1,035,905..1,042,216(-)
Genome classification: Core
Product Description: phosphoenolpyruvate carboxylase
SignalP Peptide: N/A
# Transmembrane Domains: 0
EC Numbers: 4.1.1.31 (Phosphoenolpyruvate carboxylase)
Curated GO (PlasmoDB):
| Type | GO Term | Name |
|---|---|---|
| Component | GO:0005829 | cytosol |
| Component | GO:0005634 | nucleus |
| Function | GO:0008964 | phosphoenolpyruvate carboxylase activity |
| Process | GO:0015977 | carbon fixation |
Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):
| Stage | LR class | MCA mean | MCA prop. zeros |
|---|---|---|---|
| Sporozoite | not expressed | N/A | N/A |
| Ring | expressed | 0.27 | 0.86 |
| Trophozoite | possibly expressed | 0.28 | 0.83 |
| Schizont | expressed | 0.98 | 0.49 |
| Gametocyte | expressed | 0.85 | 0.56 |
More info:
Old (Pf3D7v3) Gene ID: PF3D7_1426700
Resistome Missense Mutations: None
Resistome Compounds with Missense Mutations: None
Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)
Zhang Phenotype: Mutable in CDS
MIS: 1 | MFS: -2.966 | #Insertions: 4
PlasmoGEM Phenotype: N/A
RMgmDB ABS Phenotype: Different from wild type (Pb ortholog: PBANKA_1017900)
Modification: Disrupted | RMgm-1271
More info: PhenoPlasm Link
AlphaFill Uniprot ID: Q8ILJ7
"Best" AlphaFill ligand hit: PGA (2-phosphoglycolic acid, Local RMSD=0.29) with 6MGI (Global RMSD=3.01)
BRENDA EC Inhibitors:
| EC # | Name | EC Inhibitors |
|---|---|---|
| 4.1.1.31 | phosphoenolpyruvate carboxylase | ATPADPAMPGTPGDPCTPUTPKClNEMNaFAspGlu... |
No evidence of orthology to BindingDB entries
MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:
Homozygous genotype calls only
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 8 | 29 | 24 | 60 |
| disruptive | 17 | 24 | 16 | 78 |
| missense | 17 | 23 | 15 | 62 |
Any inclusion in genotype call
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 11 | 91 | 42 | 65 |
| disruptive | 23 | 109 | 83 | 216 |
| missense | 21 | 76 | 61 | 127 |
PlasmoDB Total SNPs: 222
Non-coding: 170 | Synonymous: 31 | Nonsynonymous: 21 | Stop Codon: 0
Protein Length: 1148 | Molecular Weight (kDa): 133.96
UniProt ID(s): Q8ILJ7
PDB ID(s): None
Isoelectric Point: 7.85
Protein Domain Annotations:
| Source | Family ID | Description |
|---|---|---|
| InterPro | IPR015813 | Pyruvate/Phosphoenolpyruvate kinase-like domain superfamily |
| InterPro | IPR021135 | Phosphoenolpyruvate carboxylase |
| InterPro | IPR033129 | Phosphoenolpyruvate carboxylase, His active site |
| PFam | PF00311 | Phosphoenolpyruvate carboxylase |
| Superfamily | SSF51621 | Pyruvate/Phosphoenolpyruvate kinase-like domain superfamily |
| PMID | Title | Authors | DOI/Link |
|---|---|---|---|
| 12368864 | Genome sequence of the human malaria parasite Plasmodium falciparum. | Gardner MJ, Hall N, ..., Barrell B | 10.1038/nature01097 |
| 16267556 | A protein interaction network of the malaria parasite Plasmodium falciparum. | LaCount DJ, Vignali M, ..., Hughes RE | 10.1038/nature04104 |
| 23181666 | Organellar proteomics reveals hundreds of novel nuclear proteins in the malariaparasite Plasmodium falciparum. | Oehring SC, Woodcroft BJ, ..., Voss TS | 10.1186/gb-2012-13-11-r108 |
| 24453970 | Phosphoenolpyruvate carboxylase identified as a key enzyme in erythrocyticPlasmodium falciparum carbon metabolism. | Storm J, Sethia S, ..., Muller S | 10.1371/journal.ppat.1003876 |