PF3D7_1427100

Genome location: Pf3D7_14_v3:1,055,788..1,060,651(+)

Genome classification: Core

Function and Localization

Product Description: lipase, putative

SignalP Peptide: N/A

# Transmembrane Domains: 1

EC Numbers: 3.1.1.3 (Triacylglycerol lipase);3.1.1.32 (Phospholipase A(1))

Curated GO (PlasmoDB): None

Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):

Stage	LR class	MCA mean	MCA prop. zeros
Sporozoite	expressed	N/A	N/A
Ring	expressed	2.01	0.27
Trophozoite	expressed	0.55	0.72
Schizont	expressed	0.39	0.78
Gametocyte	expressed	0.91	0.53

More info:

Resistome Mutations

Old (Pf3D7v3) Gene ID: PF3D7_1427100

Resistome Missense Mutations: W1039C

Resistome Compounds with Missense Mutations: Artemisinin

Resistome # Samples with Disruptive Mutations: 10 (2 missense, 2 "interesting" missense)

Essentiality (ABS)

Zhang Phenotype: Mutable in CDS

MIS: 1 | MFS: -1.144 | #Insertions: 7

PlasmoGEM Phenotype: Dispensable (Pb ortholog: PBANKA_1017500)

Relative Growth Rate: 0.87 ± 0.17
Confidence: 4.88

RMgmDB ABS Phenotype: Different from wild type (Pb ortholog: PBANKA_1017500)

Modification: Disrupted | RMgm-2802

More info: PhenoPlasm Link

Binding Evidence

AlphaFill Uniprot ID: Q8ILJ3

"Best" AlphaFill ligand hit: CXS (3-cyclohexyl-1-propylsulfonic acid, Local RMSD=0.02) with 2IX9 (Global RMSD=2.98)

BRENDA EC Inhibitors:

EC #	Name	EC Inhibitors
3.1.1.3	triacylglycerol lipase	BRENDA SOAP query unsuccessful
3.1.1.32	phospholipase A1	KClSDSDFPEDTAPMSFMgCl2CHAPSE-600cocaineInsulinTween 20tobramycin...

No evidence of orthology to BindingDB entries

Orthology Information

Ortholog Group (OrthoMCL): OG6_145929

No human ortholog(s)

Genetic Variation

MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:

Homozygous genotype calls only

variant type	common	rare	doubleton	singleton
synonymous	18	36	18	51
disruptive	73	145	57	161
missense	33	80	34	93

Any inclusion in genotype call

variant type	common	rare	doubleton	singleton
synonymous	25	90	35	46
disruptive	129	297	156	448
missense	54	166	82	144

PlasmoDB Total SNPs: 294

Non-coding: 56 | Synonymous: 155 | Nonsynonymous: 83 | Stop Codon: 0

Protein Information

Protein Length: 1320 | Molecular Weight (kDa): 155.668

UniProt ID(s): Q8ILJ3

PDB ID(s): None

Isoelectric Point: 4.74

Protein Domain Annotations:

Source	Family ID	Description
InterPro	IPR002921	Fungal lipase-like domain
InterPro	IPR029058	Alpha/Beta hydrolase fold
PFam	PF01764	Fungal lipase-like domain
Superfamily	SSF53474	Alpha/Beta hydrolase fold