Genome location: Pf3D7_14_v3:1,081,038..1,084,631(+)
Genome classification: Core
Product Description: DNA mismatch repair protein MSH2, putative
SignalP Peptide: N/A
# Transmembrane Domains: 0
EC Numbers: None
Curated GO (PlasmoDB):
| Type | GO Term | Name |
|---|---|---|
| Component | GO:0032301 | MutSalpha complex |
| Component | GO:0032302 | MutSbeta complex |
| Component | GO:0032300 | mismatch repair complex |
| Component | GO:0005634 | nucleus |
| Function | GO:0008094 | ATP-dependent activity, acting on DNA |
| Function | GO:0003677 | DNA binding |
| Function | GO:0003684 | damaged DNA binding |
| Function | GO:0000406 | double-strand/single-strand DNA junction binding |
| Process | GO:0006310 | DNA recombination |
| Process | GO:0043570 | maintenance of DNA repeat elements |
| Process | GO:0006298 | mismatch repair |
| Process | GO:0045910 | negative regulation of DNA recombination |
| Process | GO:0006301 | postreplication repair |
Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):
| Stage | LR class | MCA mean | MCA prop. zeros |
|---|---|---|---|
| Sporozoite | not expressed | N/A | N/A |
| Ring | expressed | 0.07 | 0.97 |
| Trophozoite | expressed | 0.64 | 0.64 |
| Schizont | expressed | 0.14 | 0.91 |
| Gametocyte | possibly expressed | 0.11 | 0.94 |
More info:
Old (Pf3D7v3) Gene ID: PF3D7_1427500
Resistome Missense Mutations: None
Resistome Compounds with Missense Mutations: None
Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)
Zhang Phenotype: Mutable in CDS
MIS: 1 | MFS: -0.94 | #Insertions: 3
PlasmoGEM Phenotype: Slow (Pb ortholog: PBANKA_1017100)
RMgmDB ABS Phenotype: Different from wild type (Pb ortholog: PBANKA_1017100)
Modification: Disrupted | RMgm-2801
More info: PhenoPlasm Link
AlphaFill Uniprot ID: Q8ILI9
"Best" AlphaFill ligand hit: ANP (phosphoaminophosphonic acid-adenylate ester, Local RMSD=0.21) with 5AKB (Global RMSD=2.22)
No associated EC numbersNo evidence of orthology to BindingDB entries
Ortholog Group (OrthoMCL): OG6_102031
Most Similar Human Ortholog: P43246
TM-align score: 0.88 | RMSD: 3.15
Seq Identity: 0.32 | Length: 765 / 811
All Human Orthologs (OrthoMCL):
| Gene ID | Description |
|---|---|
| ENSG00000095002 | mutS homolog 2 |
MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:
Homozygous genotype calls only
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 2 | 30 | 24 | 41 |
| disruptive | 6 | 18 | 20 | 36 |
| missense | 6 | 18 | 20 | 32 |
Any inclusion in genotype call
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 4 | 84 | 17 | 27 |
| disruptive | 12 | 42 | 27 | 62 |
| missense | 10 | 33 | 25 | 49 |
PlasmoDB Total SNPs: 62
Non-coding: 42 | Synonymous: 16 | Nonsynonymous: 4 | Stop Codon: 0
Protein Length: 811 | Molecular Weight (kDa): 94.739
PDB ID(s): None
Isoelectric Point: 7.28
Protein Domain Annotations:
| Source | Family ID | Description |
|---|---|---|
| InterPro | IPR000432 | DNA mismatch repair protein MutS, C-terminal |
| InterPro | IPR007696 | DNA mismatch repair protein MutS, core |
| InterPro | IPR011184 | DNA mismatch repair Msh2-type |
| InterPro | IPR027417 | P-loop containing nucleoside triphosphate hydrolase |
| InterPro | IPR032642 | DNA mismatch repair protein Msh2 |
| InterPro | IPR036187 | DNA mismatch repair protein MutS, core domain superfamily |
| PFam | PF00488 | DNA mismatch repair protein MutS, C-terminal |
| PFam | PF05192 | DNA mismatch repair protein MutS, core |
| Superfamily | SSF48334 | DNA mismatch repair protein MutS, core domain superfamily |
| Superfamily | SSF52540 | P-loop containing nucleoside triphosphate hydrolase |
| PMID | Title | Authors | DOI/Link |
|---|---|---|---|
| 12368864 | Genome sequence of the human malaria parasite Plasmodium falciparum. | Gardner MJ, Hall N, ..., Barrell B | 10.1038/nature01097 |
| 28232818 | Genome Wide In silico Analysis of the Mismatch Repair Components of Plasmodiumfalciparum and Their Comparison with Human Host | Tarique M, Ahmad M, Chauhan M, Tuteja R | 10.3389/fmicb.2017.00130 |
| 37083479 | Analysis of genome instability and implications for the consequent phenotype inPlasmodium falciparum containing mutated MSH2-1 (P513T) | Honma H, Takahashi N, Arisue N, Sugishita T | 10.1099/mgen.0.001003 |