PF3D7_1427600 (MIT3)

Genome location: Pf3D7_14_v3:1,084,975..1,087,482(+)

Genome classification: Core

Function and Localization

Product Description: CorA-like Mg2+ transporter protein, putative

SignalP Peptide: N/A

# Transmembrane Domains: 2

EC Numbers: None

Curated GO (PlasmoDB):

Type	GO Term	Name
Function	GO:0015095	magnesium ion transmembrane transporter activity
Process	GO:0015693	magnesium ion transport

Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):

Stage	LR class	MCA mean	MCA prop. zeros
Sporozoite	not expressed	N/A	N/A
Ring	not expressed	0.03	0.98
Trophozoite	possibly expressed	0.23	0.86
Schizont	possibly expressed	0.03	0.98
Gametocyte	not expressed	0.22	0.87

More info:

Resistome Mutations

Old (Pf3D7v3) Gene ID: PF3D7_1427600

Resistome Missense Mutations: None

Resistome Compounds with Missense Mutations: None

Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)

Essentiality (ABS)

Zhang Phenotype: Mutable in CDS

MIS: 0.409 | MFS: -3.068 | #Insertions: 1

PlasmoGEM Phenotype: Dispensable (Pb ortholog: PBANKA_1017000)

Relative Growth Rate: 1.01 ± 0.08
Confidence: 6.33

RMgmDB ABS Phenotype: Not different from wild type (Pb ortholog: PY17X_1018500)

Modification: Disrupted | RMgm-4783

More info: PhenoPlasm Link

Binding Evidence

AlphaFill Uniprot ID: Q8ILI8

"Best" AlphaFill ligand hit: GNP (phosphoaminophosphonic acid-guanylate ester, Local RMSD=13.70) with 5IQI (Global RMSD=21.21)

No associated EC numbers

No evidence of orthology to BindingDB entries

Orthology Information

Ortholog Group (OrthoMCL): OG6_102708

Most Similar Human Ortholog: Q9HD23

TM-align score: 0.64 | RMSD: 4.76

Seq Identity: 0.18 | Length: 357 / 478

All Human Orthologs (OrthoMCL):

Gene ID	Description
ENSG00000124532	magnesium transporter MRS2

Genetic Variation

MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:

Homozygous genotype calls only

variant type	common	rare	doubleton	singleton
synonymous	4	15	11	19
disruptive	2	21	8	27
missense	2	19	8	22

Any inclusion in genotype call

variant type	common	rare	doubleton	singleton
synonymous	12	37	6	16
disruptive	9	41	13	54
missense	7	32	11	39

PlasmoDB Total SNPs: 74

Non-coding: 49 | Synonymous: 19 | Nonsynonymous: 5 | Stop Codon: 1

Protein Information

Protein Length: 478 | Molecular Weight (kDa): 56.682

UniProt ID(s): Q8ILI8

PDB ID(s): None

Isoelectric Point: 6.17

Protein Domain Annotations:

Source	Family ID	Description
InterPro	IPR039204	Magnesium transporter MRS2-like
PFam	N/A	N/A
Superfamily	N/A	N/A