PF3D7_1428200 (MFS5)

Genome location: Pf3D7_14_v3:1,100,870..1,104,966(+)

Genome classification: Core

Function and Localization

Product Description: major facilitator superfamily domain-containing protein, putative

SignalP Peptide: N/A

# Transmembrane Domains: 12

EC Numbers: None

Curated GO (PlasmoDB):

Type	GO Term	Name
Process	GO:0009410	response to xenobiotic stimulus

Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):

Stage	LR class	MCA mean	MCA prop. zeros
Sporozoite	not expressed	N/A	N/A
Ring	expressed	0.84	0.64
Trophozoite	expressed	0.29	0.83
Schizont	expressed	0.55	0.69
Gametocyte	expressed	0.38	0.78

More info:

Resistome Mutations

Old (Pf3D7v3) Gene ID: PF3D7_1428200

Resistome Missense Mutations: None

Resistome Compounds with Missense Mutations: None

Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)

Essentiality (ABS)

Zhang Phenotype: Mutable in CDS

MIS: 1 | MFS: -0.985 | #Insertions: 7

PlasmoGEM Phenotype: Dispensable (Pb ortholog: PBANKA_1016400)

Relative Growth Rate: 0.87 ± 0.25
Confidence: 4.14

RMgmDB ABS Phenotype: Different from wild type (Pb ortholog: PbANKA_1016400)

Modification: Disrupted | RMgm-4339

More info: PhenoPlasm Link

Binding Evidence

AlphaFill Uniprot ID: Q8ILI3

"Best" AlphaFill ligand hit: No AlphaFill hits

No associated EC numbers

No evidence of orthology to BindingDB entries

Orthology Information

Ortholog Group (OrthoMCL): OG6_533559

No human ortholog(s)

Genetic Variation

MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:

Homozygous genotype calls only

variant type	common	rare	doubleton	singleton
synonymous	20	32	15	40
disruptive	22	78	41	125
missense	17	74	38	108

Any inclusion in genotype call

variant type	common	rare	doubleton	singleton
synonymous	25	61	30	49
disruptive	53	178	102	220
missense	37	145	86	149

PlasmoDB Total SNPs: 291

Non-coding: 185 | Synonymous: 69 | Nonsynonymous: 36 | Stop Codon: 1

Protein Information

Protein Length: 809 | Molecular Weight (kDa): 96.054

UniProt ID(s): I6Q0G4, I6Q0Z7, I6Q100, I6Q105, I6Q2L5, I6Q2L8, I6Q2L9, I6Q2M1, I6Q423, I6Q5I5, I6Q5I6, Q8ILI3

PDB ID(s): None

Isoelectric Point: 9.08

Protein Domain Annotations:

Source	Family ID	Description
InterPro	IPR011701	Major facilitator superfamily
InterPro	IPR036259	MFS transporter superfamily
PFam	PF07690	Major facilitator superfamily
Superfamily	SSF103473	MFS transporter superfamily

PMID	Title	Authors	DOI/Link
11152613	Predicting transmembrane protein topology with a hidden Markov model: applicationto complete genomes.	Krogh A, Larsson B, von Heijne G, Sonnhammer EL	10.1006/jmbi.2000.4315
12368864	Genome sequence of the human malaria parasite Plasmodium falciparum.	Gardner MJ, Hall N, ..., Barrell B	10.1038/nature01097
28236172	In-silico screening, identification and validation of a novel vaccine candidatein the fight against Plasmodium falciparum.	Panda SK, Mahapatra RK	10.1007/s00436-017-5408-z