PF3D7_1428500

Genome location: Pf3D7_14_v3:1,118,519..1,126,439(+)

Genome classification: Core

Function and Localization

Product Description: protein kinase, putative

SignalP Peptide: N/A

# Transmembrane Domains: 1

EC Numbers: 2.7.11.1 (Non-specific serine/threonine protein kinase)

Curated GO (PlasmoDB):

Type	GO Term	Name
Process	GO:0006415	translational termination

Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):

Stage	LR class	MCA mean	MCA prop. zeros
Sporozoite	not expressed	N/A	N/A
Ring	possibly expressed	1.06	0.57
Trophozoite	possibly expressed	0.48	0.74
Schizont	possibly expressed	0.25	0.86
Gametocyte	expressed	1.10	0.51

More info:

Resistome Mutations

Old (Pf3D7v3) Gene ID: PF3D7_1428500

Resistome Missense Mutations: None

Resistome Compounds with Missense Mutations: None

Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)

Essentiality (ABS)

Zhang Phenotype: Mutable in CDS

MIS: 1 | MFS: -1.442 | #Insertions: 15

PlasmoGEM Phenotype: Dispensable (Pb ortholog: PBANKA_1016200)

Relative Growth Rate: 0.93 ± 0.15
Confidence: 5.15

RMgmDB ABS Phenotype: Different from wild type (Pb ortholog: PBANKA_1016200)

Modification: Disrupted | RMgm-2796

More info: PhenoPlasm Link

Binding Evidence

AlphaFill Uniprot ID: Q8ILH9

"Best" AlphaFill ligand hit: ADP (adenosine-5'-diphosphate, Local RMSD=1.51) with 6D3K (Global RMSD=5.43)

BRENDA EC Inhibitors:

EC #	Name	EC Inhibitors
2.7.11.1	non-specific serine/threonine protein kinase	ATPKClDTTP53DRBEDTATBCAKN-93K252aCKI-7LAALSTAACS...

No evidence of orthology to BindingDB entries

Orthology Information

Ortholog Group (OrthoMCL): OG6_532699

No human ortholog(s)

Genetic Variation

MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:

Homozygous genotype calls only

variant type	common	rare	doubleton	singleton
synonymous	12	86	52	139
disruptive	42	219	143	353
missense	35	190	124	301

Any inclusion in genotype call

variant type	common	rare	doubleton	singleton
synonymous	24	182	103	141
disruptive	97	555	249	573
missense	55	439	211	400

PlasmoDB Total SNPs: 233

Non-coding: 75 | Synonymous: 107 | Nonsynonymous: 51 | Stop Codon: 0

Protein Information

Protein Length: 2353 | Molecular Weight (kDa): 282.367

UniProt ID(s): Q8ILH9

PDB ID(s): None

Isoelectric Point: 8.66

Protein Domain Annotations:

Source	Family ID	Description
InterPro	IPR011009	Protein kinase-like domain superfamily
InterPro	IPR016135	Ubiquitin-conjugating enzyme/RWD-like
PFam	N/A	N/A
Superfamily	SSF54495	Ubiquitin-conjugating enzyme/RWD-like
Superfamily	SSF56112	Protein kinase-like domain superfamily

PMID	Title	Authors	DOI/Link
12368864	Genome sequence of the human malaria parasite Plasmodium falciparum.	Gardner MJ, Hall N, ..., Barrell B	10.1038/nature01097
12368868	Sequence of Plasmodium falciparum chromosomes 2, 10, 11 and 14.	Gardner MJ, Shallom SJ, ..., Fraser CM	10.1038/nature01094