Genome location: Pf3D7_14_v3:1,126,538..1,129,101(+)
Genome classification: Core
Product Description: peptide chain release factor 1
SignalP Peptide: MKKLLFFFYCYIWFDLFVHVSNF
# Transmembrane Domains: 0
EC Numbers: 3.1.1.29 (Aminoacyl-tRNA hydrolase)
Curated GO (PlasmoDB):
| Type | GO Term | Name |
|---|---|---|
| Component | GO:0020011 | apicoplast |
| Component | GO:0005739 | mitochondrion |
| Function | GO:0043022 | ribosome binding |
| Function | GO:0003747 | translation release factor activity |
| Process | GO:0006415 | translational termination |
Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):
| Stage | LR class | MCA mean | MCA prop. zeros |
|---|---|---|---|
| Sporozoite | not expressed | N/A | N/A |
| Ring | not expressed | 0.02 | 0.99 |
| Trophozoite | expressed | 0.17 | 0.88 |
| Schizont | expressed | 0.07 | 0.95 |
| Gametocyte | expressed | 0.25 | 0.85 |
More info:
Old (Pf3D7v3) Gene ID: PF3D7_1428600
Resistome Missense Mutations: None
Resistome Compounds with Missense Mutations: None
Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)
Zhang Phenotype: Non - Mutable in CDS
MIS: 0.615 | MFS: -2.828 | #Insertions: 0
PlasmoGEM Phenotype: Dispensable (Pb ortholog: PBANKA_1016100)
RMgmDB ABS Phenotype: Different from wild type (Pb ortholog: PBANKA_1016100)
Modification: Disrupted | RMgm-2795
More info: PhenoPlasm Link
AlphaFill Uniprot ID: Q8ILH8
"Best" AlphaFill ligand hit: ADE (adenine, Local RMSD=0.22) with 4L0M (Global RMSD=18.67)
BRENDA EC Inhibitors:
| EC # | Name | EC Inhibitors |
|---|---|---|
| 3.1.1.29 | aminoacyl-tRNA hydrolase | EDTAtRNAtRNALystRNAfMettRNA-lysine5-azacytidine70 S ribosomeUncharged tRNAdeacetylated tRNAdiethyldicarbonatenon-esterified tRNALystRNA-formyl-methionine... |
No evidence of orthology to BindingDB entries
Ortholog Group (OrthoMCL): OG6_101033
Most Similar Human Ortholog: Q5TF45
TM-align score: 0.54 | RMSD: 4.59
Seq Identity: 0.36 | Length: 139 / 304
All Human Orthologs (OrthoMCL):
| Gene ID | Description |
|---|---|
| ENSG00000112031 | mitochondrial translational release factor 1 like |
MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:
Homozygous genotype calls only
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 2 | 9 | 8 | 18 |
| disruptive | 6 | 23 | 12 | 38 |
| missense | 6 | 21 | 11 | 32 |
Any inclusion in genotype call
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 2 | 26 | 15 | 20 |
| disruptive | 12 | 52 | 28 | 73 |
| missense | 8 | 43 | 25 | 53 |
PlasmoDB Total SNPs: 129
Non-coding: 115 | Synonymous: 10 | Nonsynonymous: 4 | Stop Codon: 0
Protein Length: 304 | Molecular Weight (kDa): 36.437
UniProt ID(s): Q8ILH8
PDB ID(s): None
Isoelectric Point: 10.23
Protein Domain Annotations:
| Source | Family ID | Description |
|---|---|---|
| InterPro | IPR000352 | Peptide chain release factor class I |
| InterPro | IPR005139 | Peptide chain release factor |
| PFam | PF00472 | Peptide chain release factor class I |
| PFam | PF03462 | Peptide chain release factor |
| Superfamily | SSF75620 | N/A |
| PMID | Title | Authors | DOI/Link |
|---|---|---|---|
| 12368864 | Genome sequence of the human malaria parasite Plasmodium falciparum. | Gardner MJ, Hall N, ..., Barrell B | 10.1038/nature01097 |
| 26946524 | Polypeptide release factors and stop codon recognition in the apicoplast andmitochondrion of Plasmodium falciparum. | Vaishya S, Kumar V, ..., Habib S | 10.1111/mmi.13369 |
| 30212465 | Integrative proteomics and bioinformatic prediction enable a high-confidenceapicoplast proteome in malaria parasites. | Boucher MJ, Ghosh S, ..., Yeh E | 10.1371/journal.pbio.2005895 |