PF3D7_1429000

Genome location: Pf3D7_14_v3:1,140,579..1,141,292(-)

Genome classification: Core

Function and Localization

Product Description: protein archease, putative

SignalP Peptide: N/A

# Transmembrane Domains: 0

EC Numbers: None

Curated GO (PlasmoDB):

Type	GO Term	Name
Component	GO:0072669	tRNA-splicing ligase complex
Process	GO:0006388	tRNA splicing, via endonucleolytic cleavage and ligation

Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):

Stage	LR class	MCA mean	MCA prop. zeros
Sporozoite	not expressed	N/A	N/A
Ring	not expressed	0.08	0.96
Trophozoite	not expressed	0.23	0.84
Schizont	not expressed	0.05	0.96
Gametocyte	not expressed	0.19	0.88

More info:

Resistome Mutations

Old (Pf3D7v3) Gene ID: PF3D7_1429000

Resistome Missense Mutations: None

Resistome Compounds with Missense Mutations: None

Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)

Essentiality (ABS)

Zhang Phenotype: Non - Mutable in CDS

MIS: 0.119 | MFS: -2.982 | #Insertions: 0

PlasmoGEM Phenotype: Essential (Pb ortholog: PBANKA_1015700)

Relative Growth Rate: 0.06 ± 0.05
Confidence: 7.33

RMgmDB ABS Phenotype: N/A

More info: PhenoPlasm Link

Binding Evidence

AlphaFill Uniprot ID: Q8ILH4

"Best" AlphaFill ligand hit: No AlphaFill hits

No associated EC numbers

No evidence of orthology to BindingDB entries

Orthology Information

Ortholog Group (OrthoMCL): OG6_103121

Most Similar Human Ortholog: D3DPQ2

TM-align score: 0.95 | RMSD: 0.82

Seq Identity: 0.30 | Length: 109 / 237

All Human Orthologs (OrthoMCL):

Gene ID	Description
ENSG00000176261	zinc finger and BTB domain containing 8 opposite strand

Genetic Variation

MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:

Homozygous genotype calls only

variant type	common	rare	doubleton	singleton
synonymous	3	11	2	9
disruptive	11	34	18	37
missense	4	19	17	27

Any inclusion in genotype call

variant type	common	rare	doubleton	singleton
synonymous	7	18	8	12
disruptive	21	72	27	64
missense	9	46	20	46

PlasmoDB Total SNPs: 29

Non-coding: 0 | Synonymous: 17 | Nonsynonymous: 11 | Stop Codon: 1

Protein Information

Protein Length: 237 | Molecular Weight (kDa): 28.074

UniProt ID(s): Q8ILH4

PDB ID(s): None

Isoelectric Point: 4.73

Protein Domain Annotations:

Source	Family ID	Description
InterPro	IPR002804	Archease
InterPro	IPR023572	Archease domain
InterPro	IPR036820	Archease domain superfamily
PFam	PF01951	Archease domain
Superfamily	SSF69819	Archease domain superfamily