PF3D7_1429400

Genome location: Pf3D7_14_v3:1,154,726..1,163,789(+)

Genome classification: Core

Function and Localization

Product Description: rRNA (adenosine-2'-O-)-methyltransferase, putative

SignalP Peptide: N/A

# Transmembrane Domains: 0

EC Numbers: 2.1.1.- (Methyltransferases.)

Curated GO (PlasmoDB):

Type	GO Term	Name
Function	GO:0016423	tRNA (guanine) methyltransferase activity
Process	GO:0030488	tRNA methylation

Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):

Stage	LR class	MCA mean	MCA prop. zeros
Sporozoite	not expressed	N/A	N/A
Ring	expressed	0.56	0.75
Trophozoite	expressed	0.80	0.58
Schizont	possibly expressed	0.21	0.87
Gametocyte	possibly expressed	0.63	0.66

More info:

Resistome Mutations

Old (Pf3D7v3) Gene ID: PF3D7_1429400

Resistome Missense Mutations: T2041K

Resistome Compounds with Missense Mutations: AN13762

Resistome # Samples with Disruptive Mutations: 1 (1 missense, 1 "interesting" missense)

Essentiality (ABS)

Zhang Phenotype: Mutable in CDS

MIS: 1 | MFS: -1.261 | #Insertions: 13

PlasmoGEM Phenotype: Dispensable (Pb ortholog: PBANKA_1015300)

Relative Growth Rate: 0.77 ± 0.31
Confidence: 3.76

RMgmDB ABS Phenotype: Different from wild type (Pb ortholog: PBANKA_1015300)

Modification: Disrupted | RMgm-2790

More info: PhenoPlasm Link

Binding Evidence

AlphaFill Uniprot ID: N/A

"Best" AlphaFill ligand hit: No AlphaFill hits

BRENDA EC Inhibitors:

EC #	Name	EC Inhibitors
2.1.1.-	Ex: homocysteine S-methyltransferase	EDTACTABNickelA9145CtylosintacrineTween 80FTI-2628macrocinadenosineRelomycinphosphate...

No evidence of orthology to BindingDB entries

Orthology Information

Ortholog Group (OrthoMCL): OG6_102883

Most Similar Human Ortholog: Not available

TM-align score: 0.00 | RMSD: 0.00

Seq Identity: 0.00 | Length: / 2779

All Human Orthologs (OrthoMCL):

Gene ID	Description
ENSG00000059588	TAR (HIV-1) RNA binding protein 1

Genetic Variation

MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:

Homozygous genotype calls only

variant type	common	rare	doubleton	singleton
synonymous	33	118	78	135
disruptive	167	393	222	483
missense	95	314	186	419

Any inclusion in genotype call

variant type	common	rare	doubleton	singleton
synonymous	50	241	92	157
disruptive	264	832	318	637
missense	154	650	260	479

PlasmoDB Total SNPs: 518

Non-coding: 65 | Synonymous: 235 | Nonsynonymous: 213 | Stop Codon: 5

Protein Information

Protein Length: 2779 | Molecular Weight (kDa): 332.897

UniProt ID(s): Q8ILH0

PDB ID(s): None

Isoelectric Point: 8.51

Protein Domain Annotations:

Source	Family ID	Description
InterPro	IPR001537	tRNA/rRNA methyltransferase, SpoU type
InterPro	IPR029028	Alpha/beta knot methyltransferases
PFam	PF00588	tRNA/rRNA methyltransferase, SpoU type
Superfamily	SSF57997	Alpha/beta knot methyltransferases
Superfamily	SSF75217