Genome location: Pf3D7_14_v3:1,176,022..1,181,728(+)
Genome classification: Core
Product Description: ADP-dependent DNA helicase RecQ
SignalP Peptide: N/A
# Transmembrane Domains: 0
EC Numbers: 3.6.4.12 (DNA helicase)
Curated GO (PlasmoDB):
| Type | GO Term | Name |
|---|---|---|
| Component | GO:0005694 | chromosome |
| Component | GO:0000781 | chromosome, telomeric region |
| Component | GO:0005737 | cytoplasm |
| Component | GO:0005634 | nucleus |
| Function | GO:0043138 | 3'-5' DNA helicase activity |
| Function | GO:0003678 | DNA helicase activity |
| Function | GO:0009378 | four-way junction helicase activity |
| Function | GO:0005515 | protein binding |
| Process | GO:0032508 | DNA duplex unwinding |
| Process | GO:0006310 | DNA recombination |
| Process | GO:0006281 | DNA repair |
| Process | GO:0006268 | DNA unwinding involved in DNA replication |
| Process | GO:0000724 | double-strand break repair via homologous recombination |
Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):
| Stage | LR class | MCA mean | MCA prop. zeros |
|---|---|---|---|
| Sporozoite | expressed | N/A | N/A |
| Ring | not expressed | 0.18 | 0.91 |
| Trophozoite | possibly expressed | 0.61 | 0.65 |
| Schizont | expressed | 0.54 | 0.67 |
| Gametocyte | expressed | 0.60 | 0.66 |
More info:
Old (Pf3D7v3) Gene ID: PF3D7_1429900
Resistome Missense Mutations: None
Resistome Compounds with Missense Mutations: None
Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)
Zhang Phenotype: Mutable in CDS
MIS: 1 | MFS: -2.509 | #Insertions: 3
PlasmoGEM Phenotype: Slow (Pb ortholog: PBANKA_1014800)
RMgmDB ABS Phenotype: Different from wild type (Pb ortholog: PBANKA_1014800)
Modification: Disrupted | RMgm-2786
More info: PhenoPlasm Link
AlphaFill Uniprot ID: Q8ILG5
"Best" AlphaFill ligand hit: AMP (adenosine monophosphate, Local RMSD=0.03) with 4KBF (Global RMSD=2.20)
BRENDA EC Inhibitors:
| EC # | Name | EC Inhibitors |
|---|---|---|
| 3.6.4.12 | DNA helicase | No BRENDA inhibitors |
Orthology to BindingDB Entries:
| UniProt | Protein Name | Species | Homology Source | BindingDB Ligands |
|---|---|---|---|---|
| Q14191 | Bifunctional 3'-5' exonuclease/ATP-dependent helicase WRN | Homo sapiens | OrthoDB, OrthoMCL BLAST | CHEMBL2426168CHEMBL1374426MLS000559245CHEMBL2426190... |
| P54132 | RecQ-like DNA helicase BLM | Homo sapiens | OrthoDB | CHEMBL2426168MLS000559245CHEMBL2426200CHEMBL2426179... |
Ortholog Group (OrthoMCL): OG6_106943
Most Similar Human Ortholog: Q14191
TM-align score: 0.40 | RMSD: 5.59
Seq Identity: 0.27 | Length: 649 / 1440
All Human Orthologs (OrthoMCL):
| Gene ID | Description |
|---|---|
| ENSG00000165392 | WRN RecQ like helicase |
Protein Length: 1440 | Molecular Weight (kDa): 169.541
UniProt ID(s): A0A0A1E5G7, Q8ILG5
PDB ID(s): None
Isoelectric Point: 8.13
Protein Domain Annotations:
| Source | Family ID | Description |
|---|---|---|
| InterPro | IPR001650 | Helicase, C-terminal |
| InterPro | IPR004589 | DNA helicase, ATP-dependent, RecQ type |
| InterPro | IPR010997 | HRDC-like superfamily |
| InterPro | IPR011545 | DEAD/DEAH box helicase domain |
| InterPro | IPR014001 | Helicase superfamily 1/2, ATP-binding domain |
| InterPro | IPR018982 | RQC domain |
| InterPro | IPR027417 | P-loop containing nucleoside triphosphate hydrolase |
| InterPro | IPR032284 | ATP-dependent DNA helicase RecQ, zinc-binding domain |
| InterPro | IPR036390 | Winged helix DNA-binding domain superfamily |
| PFam | PF00270 | DEAD/DEAH box helicase domain |
| PFam | PF00271 | Helicase, C-terminal |
| PFam | PF09382 | RQC domain |
| PFam | PF16124 | ATP-dependent DNA helicase RecQ, zinc-binding domain |
| Superfamily | SSF46785 | Winged helix DNA-binding domain superfamily |
| Superfamily | SSF47819 | HRDC-like superfamily |
| Superfamily | SSF52540 | P-loop containing nucleoside triphosphate hydrolase |
MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:
Homozygous genotype calls only
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 14 | 49 | 35 | 63 |
| disruptive | 68 | 151 | 75 | 181 |
| missense | 38 | 97 | 58 | 145 |
Any inclusion in genotype call
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 25 | 103 | 47 | 88 |
| disruptive | 115 | 264 | 165 | 309 |
| missense | 59 | 174 | 130 | 236 |
PlasmoDB Total SNPs: 282
Non-coding: 116 | Synonymous: 86 | Nonsynonymous: 79 | Stop Codon: 1
| PMID | Title | Authors | DOI/Link |
|---|---|---|---|
| 12368864 | Genome sequence of the human malaria parasite Plasmodium falciparum. | Gardner MJ, Hall N, ..., Barrell B | 10.1038/nature01097 |
| 25824666 | Plasmodium falciparum Werner homologue is a nuclear protein and its biochemicalactivities reside in the N-terminal region. | Rahman F, Tarique M, Ahmad M, Tuteja R | 10.1007/s00709-015-0785-6 |
| 28373555 | Plasmodium falciparum erythrocyte-binding antigen 175 triggers a biophysicalchange in the red blood cell that facilitates invasion. | Koch M, Wright KE, ..., Baum J | 10.1073/pnas.1620843114 |
| 29965959 | RecQ helicases in the malaria parasite Plasmodium falciparum affect genomestability, gene expression patterns and DNA replication dynamics. | Claessens A, Harris LM, ..., Merrick CJ | 10.1371/journal.pgen.1007490 |
| 33241842 | Elucidation of an essential function of the unique charged domain of Plasmodiumtopoisomerase I II | Bansod S, Bung N, ..., Bhattacharyya S | 10.1042/BCJ20200318 |