Genome location: Pf3D7_14_v3:1,184,314..1,187,350(+)
Genome classification: Core
Product Description: serine/threonine protein phosphatase 2A activator
SignalP Peptide: N/A
# Transmembrane Domains: 0
EC Numbers: 5.2.1.8 (Peptidylprolyl isomerase)
Curated GO (PlasmoDB):
| Type | GO Term | Name |
|---|---|---|
| Component | GO:0005737 | cytoplasm |
| Component | GO:0005634 | nucleus |
| Component | GO:0000159 | protein phosphatase type 2A complex |
| Function | GO:0003755 | peptidyl-prolyl cis-trans isomerase activity |
| Function | GO:0051721 | protein phosphatase 2A binding |
| Function | GO:0019888 | protein phosphatase regulator activity |
| Function | GO:0008160 | protein tyrosine phosphatase activator activity |
| Process | GO:0007052 | mitotic spindle organization |
Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):
| Stage | LR class | MCA mean | MCA prop. zeros |
|---|---|---|---|
| Sporozoite | expressed | N/A | N/A |
| Ring | possibly expressed | 0.02 | 0.99 |
| Trophozoite | expressed | 0.08 | 0.95 |
| Schizont | expressed | 0.02 | 0.99 |
| Gametocyte | expressed | 0.46 | 0.75 |
More info:
Old (Pf3D7v3) Gene ID: PF3D7_1430100
Resistome Missense Mutations: None
Resistome Compounds with Missense Mutations: None
Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)
Zhang Phenotype: Non - Mutable in CDS
MIS: 0.476 | MFS: -2.479 | #Insertions: 0
PlasmoGEM Phenotype: N/A
RMgmDB ABS Phenotype: N/A
More info: PhenoPlasm Link
AlphaFill Uniprot ID: Q8ILG3
"Best" AlphaFill ligand hit: AGS (phosphothiophosphoric acid-adenylate ester, Local RMSD=0.42) with 4LAC (Global RMSD=3.81)
BRENDA EC Inhibitors:
| EC # | Name | EC Inhibitors |
|---|---|---|
| 5.2.1.8 | peptidylprolyl isomerase | ATPNEMSDSPCMBFK506QAEGPKAla-ProjugloneSer-ProGPI-1046RNase T1Rapamycin... |
Orthology to BindingDB Entries:
Ortholog Group (OrthoMCL): OG6_101438
Most Similar Human Ortholog: F6WIT2
TM-align score: 0.94 | RMSD: 1.39
Seq Identity: 0.34 | Length: 282 / 319
All Human Orthologs (OrthoMCL):
| Gene ID | Description |
|---|---|
| ENSG00000119383 | protein phosphatase 2 phosphatase activator |
| ENSG00000235007 | novel protein |
Protein Length: 319 | Molecular Weight (kDa): 37.442
UniProt ID(s): Q8ILG3
PDB ID(s): None
Isoelectric Point: 6.69
Protein Domain Annotations:
| Source | Family ID | Description |
|---|---|---|
| InterPro | IPR004327 | Phosphotyrosyl phosphatase activator, PTPA |
| InterPro | IPR037218 | PTPA superfamily |
| PFam | PF03095 | Phosphotyrosyl phosphatase activator, PTPA |
| Superfamily | SSF140984 | PTPA superfamily |
MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:
Homozygous genotype calls only
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 1 | 13 | 8 | 17 |
| disruptive | 0 | 12 | 8 | 28 |
| missense | 0 | 12 | 7 | 24 |
Any inclusion in genotype call
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 3 | 22 | 12 | 23 |
| disruptive | 3 | 35 | 19 | 50 |
| missense | 1 | 28 | 15 | 37 |
PlasmoDB Total SNPs: 303
Non-coding: 288 | Synonymous: 13 | Nonsynonymous: 2 | Stop Codon: 0
| PMID | Title | Authors | DOI/Link |
|---|---|---|---|
| 12368864 | Genome sequence of the human malaria parasite Plasmodium falciparum. | Gardner MJ, Hall N, ..., Barrell B | 10.1038/nature01097 |
| 16267556 | A protein interaction network of the malaria parasite Plasmodium falciparum. | LaCount DJ, Vignali M, ..., Hughes RE | 10.1038/nature04104 |
| 24521882 | PhosphoTyrosyl phosphatase activator of Plasmodium falciparum: identification ofits residues involved in binding to and activation of PP2A. | Vandomme A, Freville A, ..., Pierrot C | 10.3390/ijms15022431 |
| 37310980 | Recombinant expression and preliminary characterization of Peptidyl-prolylcis/trans-isomerase Rrd1 from Saccharomyces cerevisiae | Kashif M, Alsaiari AA, ..., Akhtar MS | 10.1371/journal.pone.0282749 |
| 37657276 | Exploring the role of the protein tyrosine kinase a (PtkA) in mycobacterial intracellular survival | Jaiswal S, Kumar S, Velarde de la Cruz E | 10.1016/j.tube.2023.102398 |
| 37711306 | ALA induces stomatal opening through regulation among PTPA, PP2AC, and SnRK2.6 | Chen Z, Zhang J, Wang L | 10.3389/fpls.2023.1206728 |
| 37754203 | Shikonin and Juglone Inhibit Mycobacterium tuberculosis Low-Molecular-WeightProtein Tyrosine Phosphatase a (Mt-PTPa) | Sulyman AO, Fulcher J, ..., Olorunniji FJ | 10.3390/biotech12030059 |
| 37424790 | Characteristics of Mycobacterium tuberculosis PtpA interaction and activity onthe alpha subunit of human mitochondrial trifunctional protein, a key enzyme oflipid metabolism | Margenat M, Betancour G, ..., Villarino A | 10.3389/fcimb.2023.1095060 |