Genome location: Pf3D7_14_v3:1,187,398..1,191,764(+)
Genome classification: Core
Product Description: plasmepsin IX
SignalP Peptide: N/A
# Transmembrane Domains: 0
EC Numbers: 3.4.23.- (Aspartic endopeptidases.)
Curated GO (PlasmoDB):
| Type | GO Term | Name |
|---|---|---|
| Component | GO:0020008 | rhoptry |
| Function | GO:0004190 | aspartic-type endopeptidase activity |
| Process | GO:0044409 | entry into host |
| Process | GO:0006508 | proteolysis |
| Process | GO:0009410 | response to xenobiotic stimulus |
Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):
| Stage | LR class | MCA mean | MCA prop. zeros |
|---|---|---|---|
| Sporozoite | expressed | N/A | N/A |
| Ring | possibly expressed | 0.05 | 0.98 |
| Trophozoite | expressed | 0.29 | 0.84 |
| Schizont | expressed | 1.29 | 0.42 |
| Gametocyte | expressed | 1.23 | 0.54 |
More info:
Old (Pf3D7v3) Gene ID: PF3D7_1430200
Resistome Missense Mutations: None
Resistome Compounds with Missense Mutations: None
Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)
Zhang Phenotype: Non - Mutable in CDS
MIS: 0.238 | MFS: -2.729 | #Insertions: 0
PlasmoGEM Phenotype: Essential (Pb ortholog: PBANKA_1222500)
RMgmDB ABS Phenotype: N/A
More info: PhenoPlasm Link
Protein Length: 627 | Molecular Weight (kDa): 74.183
PDB ID(s): None
Isoelectric Point: 9.62
Protein Domain Annotations:
| Source | Family ID | Description |
|---|---|---|
| InterPro | IPR001461 | Aspartic peptidase A1 family |
| InterPro | IPR021109 | Aspartic peptidase domain superfamily |
| InterPro | IPR033121 | Peptidase family A1 domain |
| InterPro | IPR034164 | Pepsin-like domain |
| PFam | PF00026 | Peptidase family A1 domain |
| Superfamily | SSF50630 | Aspartic peptidase domain superfamily |
MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:
Homozygous genotype calls only
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 3 | 29 | 15 | 26 |
| disruptive | 3 | 40 | 28 | 89 |
| missense | 3 | 39 | 28 | 85 |
Any inclusion in genotype call
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 9 | 53 | 20 | 43 |
| disruptive | 18 | 122 | 69 | 134 |
| missense | 10 | 103 | 60 | 105 |
PlasmoDB Total SNPs: 392
Non-coding: 345 | Synonymous: 33 | Nonsynonymous: 14 | Stop Codon: 0
| PMID | Title | Authors | DOI/Link |
|---|---|---|---|
| 12368864 | Genome sequence of the human malaria parasite Plasmodium falciparum. | Gardner MJ, Hall N, ..., Barrell B | 10.1038/nature01097 |
| 29074774 | Plasmepsins IX and X are essential and druggable mediators of malaria parasiteegress and invasion. | Nasamu AS, Glushakova S, ..., Goldberg DE | 10.1126/science.aan1478 |
| 32109369 | Dual Plasmepsin-Targeting Antimalarial Agents Disrupt Multiple Stages of theMalaria Parasite Life Cycle. | Favuzza P, de Lera Ruiz M, ..., Cowman AF | 10.1016/j.chom.2020.02.005 |
| 35460613 | Basis for drug selectivity of plasmepsin IX and X inhibition in Plasmodiumfalciparum and vivax | Hodder AN, Christensen J, ..., Cowman AF | 10.1016/j.str.2022.03.018 |
| 38179191 | Design, synthesis and modelling of photoreactive chemical probes forinvestigating target engagement of plasmepsin IX and X in Plasmodium falciparum | Lisauskaite M, Nixon GL, ..., O'Neill PM | 10.1039/d3cb00109a |
| 29074775 | A multistage antimalarial targets the plasmepsins IX and X essential for invasionand egress | Pino P, Caldelari R, ..., Soldati-Favre D | 10.1126/science.aaf8675 |
| 36385924 | The Invention of WM382, a Highly Potent PMIX/X Dual Inhibitor toward theTreatment of Malaria | de Lera Ruiz M, Favuzza P, ..., McCauley JA | 10.1021/acsmedchemlett.2c00355 |
| 36432016 | Bridging the Gap in Malaria Parasite Resistance, Current Interventions, and theWay Forward from in Silico Perspective: A Review | Kumi RO, Oti B, ..., Soliman MES | 10.3390/molecules27227915 |