PF3D7_1430400 (ATG5)

Genome location: Pf3D7_14_v3:1,200,622..1,204,458(+)

Genome classification: Core

Function and Localization

Product Description: autophagy protein 5, putative

SignalP Peptide: N/A

# Transmembrane Domains: 0

EC Numbers: None

Curated GO (PlasmoDB):

Type	GO Term	Name
Component	GO:0034274	Atg12-Atg5-Atg16 complex
Component	GO:0034045	phagophore assembly site membrane
Function	GO:0005515	protein binding
Process	GO:0006501	C-terminal protein lipidation
Process	GO:0000045	autophagosome assembly
Process	GO:0000422	autophagy of mitochondrion
Process	GO:0044804	autophagy of nucleus
Process	GO:0006995	cellular response to nitrogen starvation

Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):

Stage	LR class	MCA mean	MCA prop. zeros
Sporozoite	possibly expressed	N/A	N/A
Ring	expressed	0.11	0.94
Trophozoite	expressed	0.33	0.79
Schizont	expressed	0.05	0.97
Gametocyte	expressed	0.51	0.71

More info:

Resistome Mutations

Old (Pf3D7v3) Gene ID: PF3D7_1430400

Resistome Missense Mutations: None

Resistome Compounds with Missense Mutations: None

Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)

Essentiality (ABS)

Zhang Phenotype: Non - Mutable in CDS

MIS: 0.208 | MFS: -2.864 | #Insertions: 0

PlasmoGEM Phenotype: Essential (Pb ortholog: PBANKA_1014300)

Relative Growth Rate: 0.13 ± 0.14
Confidence: 5.30

RMgmDB ABS Phenotype: N/A

More info: PhenoPlasm Link

Binding Evidence

AlphaFill Uniprot ID: Q8ILG0

"Best" AlphaFill ligand hit: PC (phosphocholine, Local RMSD=0.12) with 2CKQ (Global RMSD=28.56)

No associated EC numbers

No evidence of orthology to BindingDB entries

Orthology Information

Ortholog Group (OrthoMCL): OG6_532495

No human ortholog(s)

Genetic Variation

MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:

Homozygous genotype calls only

variant type	common	rare	doubleton	singleton
synonymous	8	43	19	44
disruptive	35	115	58	158
missense	17	72	41	123

Any inclusion in genotype call

variant type	common	rare	doubleton	singleton
synonymous	19	72	24	52
disruptive	69	260	111	238
missense	32	175	87	119

PlasmoDB Total SNPs: 233

Non-coding: 86 | Synonymous: 95 | Nonsynonymous: 44 | Stop Codon: 8

Protein Information

Protein Length: 863 | Molecular Weight (kDa): 102.23

UniProt ID(s): Q8ILG0

PDB ID(s): None

Isoelectric Point: 6.18

Protein Domain Annotations:

Source	Family ID	Description
InterPro	IPR007239	Autophagy-related protein 5
PFam	PF04106	Autophagy-related protein 5
Superfamily	N/A	N/A

PMID	Title	Authors	DOI/Link
12368864	Genome sequence of the human malaria parasite Plasmodium falciparum.	Gardner MJ, Hall N, ..., Barrell B	10.1038/nature01097
30911187	Evolution from covalent conjugation to non-covalent interaction in theubiquitin-like ATG12 system.	Pang Y, Yamamoto H, ..., Mizushima N	10.1038/s41594-019-0204-3