Product Description: endonuclease/exonuclease/phosphatase family protein, putative
SignalP Peptide: N/A
# Transmembrane Domains: 0
EC Numbers: 3.1.16.1 (Spleen exonuclease)
Curated GO (PlasmoDB):
| Type | GO Term | Name |
|---|---|---|
| Component | GO:0005739 | mitochondrion |
| Component | GO:0005634 | nucleus |
| Function | GO:0003906 | DNA-(apurinic or apyrimidinic site) endonuclease activity |
| Function | GO:0004536 | deoxyribonuclease activity |
| Function | GO:0008311 | double-stranded DNA 3'-5' exodeoxyribonuclease activity |
| Function | GO:0004528 | phosphodiesterase I activity |
| Process | GO:0006284 | base-excision repair |
Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):
| Stage | LR class | MCA mean | MCA prop. zeros |
|---|---|---|---|
| Sporozoite | not expressed | N/A | N/A |
| Ring | not expressed | 0.08 | 0.96 |
| Trophozoite | expressed | 0.53 | 0.70 |
| Schizont | possibly expressed | 0.26 | 0.84 |
| Gametocyte | expressed | 0.42 | 0.77 |
More info:
Old (Pf3D7v3) Gene ID: PF3D7_1430600
Resistome Missense Mutations: None
Resistome Compounds with Missense Mutations: None
Resistome # Samples with Disruptive Mutations: 1 (0 missense, 0 "interesting" missense)
Zhang Phenotype: Mutable in CDS
MIS: 1 | MFS: -0.386 | #Insertions: 4
PlasmoGEM Phenotype: Dispensable (Pb ortholog: PBANKA_1014100)
RMgmDB ABS Phenotype: Different from wild type (Pb ortholog: PBANKA_1014100)
Modification: Disrupted | RMgm-2781
More info: PhenoPlasm Link
AlphaFill Uniprot ID: Q8ILF8
"Best" AlphaFill ligand hit: BU1 (1,4-butanediol, Local RMSD=0.02) with 6WCD (Global RMSD=4.77)
BRENDA EC Inhibitors:
| EC # | Name | EC Inhibitors |
|---|---|---|
| 3.1.16.1 | spleen exonuclease | ATPKClEDTARNAiheparinarseniteATPgammaSUDP-glucosePentamidineacetylationSP-ATPalphaSSP-CTPalphaS... |
No evidence of orthology to BindingDB entries
MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:
Homozygous genotype calls only
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 3 | 23 | 21 | 35 |
| disruptive | 17 | 99 | 66 | 158 |
| missense | 14 | 84 | 59 | 133 |
Any inclusion in genotype call
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 7 | 54 | 23 | 59 |
| disruptive | 61 | 201 | 119 | 187 |
| missense | 33 | 163 | 103 | 139 |
PlasmoDB Total SNPs: 90
Non-coding: 2 | Synonymous: 51 | Nonsynonymous: 37 | Stop Codon: 0
Protein Length: 876 | Molecular Weight (kDa): 104.607
UniProt ID(s): Q8ILF8
PDB ID(s): None
Isoelectric Point: 8.8
Protein Domain Annotations:
| Source | Family ID | Description |
|---|---|---|
| InterPro | IPR004808 | AP endonuclease 1 |
| InterPro | IPR005135 | Endonuclease/exonuclease/phosphatase |
| InterPro | IPR036691 | Endonuclease/exonuclease/phosphatase superfamily |
| PFam | PF03372 | Endonuclease/exonuclease/phosphatase |
| Superfamily | SSF56219 | Endonuclease/exonuclease/phosphatase superfamily |
| PMID | Title | Authors | DOI/Link |
|---|---|---|---|
| 12368864 | Genome sequence of the human malaria parasite Plasmodium falciparum. | Gardner MJ, Hall N, ..., Barrell B | 10.1038/nature01097 |
| 34494883 | A Prioritized and Validated Resource of Mitochondrial Proteins in PlasmodiumIdentifies Unique Biology. | van Esveld SL, Meerstein-Kessel L, ..., Huynen MA | 10.1128/mSphere.00614-21 |