Genome location: Pf3D7_14_v3:1,210,949..1,213,299(+)
Genome classification: Core
Product Description: NADP-specific glutamate dehydrogenase
SignalP Peptide: MILYSCVVCFIVFVFHVKAYS
# Transmembrane Domains: 0
EC Numbers: 1.4.1.4 (Glutamate dehydrogenase (NADP(+)))
Curated GO (PlasmoDB):
| Type | GO Term | Name |
|---|---|---|
| Component | GO:0020011 | apicoplast |
| Component | GO:0009986 | cell surface |
| Component | GO:0005829 | cytosol |
| Function | GO:0004354 | glutamate dehydrogenase (NADP+) activity |
| Process | GO:0006520 | cellular amino acid metabolic process |
| Process | GO:0006537 | glutamate biosynthetic process |
Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):
| Stage | LR class | MCA mean | MCA prop. zeros |
|---|---|---|---|
| Sporozoite | expressed | N/A | N/A |
| Ring | possibly expressed | 0.02 | 0.99 |
| Trophozoite | expressed | 0.15 | 0.90 |
| Schizont | expressed | 0.02 | 0.98 |
| Gametocyte | expressed | 0.05 | 0.97 |
More info:
Old (Pf3D7v3) Gene ID: PF3D7_1430700
Resistome Missense Mutations: None
Resistome Compounds with Missense Mutations: None
Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)
Zhang Phenotype: Non - Mutable in CDS
MIS: 0.125 | MFS: -2.934 | #Insertions: 0
PlasmoGEM Phenotype: Insufficient data (Pb ortholog: PBANKA_1026200)
RMgmDB ABS Phenotype: Different from wild type (Pb ortholog: PBANKA_1026200)
Modification: Disrupted | RMgm-2841
More info: PhenoPlasm Link
AlphaFill Uniprot ID: Q8ILF7
"Best" AlphaFill ligand hit: ADP (adenosine-5'-diphosphate, Local RMSD=0.30) with 6DHK (Global RMSD=2.59)
BRENDA EC Inhibitors:
| EC # | Name | EC Inhibitors |
|---|---|---|
| 1.4.1.4 | glutamate dehydrogenase (NADP+) | O2ATPADPAMPKClNAD+NADHEDTANH4+NADPHNADP+HgCl2... |
Orthology to BindingDB Entries:
| UniProt | Protein Name | Species | Homology Source | BindingDB Ligands |
|---|---|---|---|---|
| P49448 | Glutamate dehydrogenase 2, mitochondrial | OrthoDB, OrthoMCL BLAST | US8604016, 1US8604016, 1US8604016, 295US8604016, 295... | |
| P00366 | Glutamate dehydrogenase 1, mitochondrial | Bos taurus | OrthoDB, OrthoMCL BLAST | [(2R,3S,4R,5R)-5-(6-amino-9H-purin-9-yl)-3,4-dihydroxyoxolan-2-yl]methyl ({[(3R,4S)-5-(3-carbamoyl-1,4-dihydropyridin-1-yl)-3,4-dihydroxyoxolan-2-yl]methyl phosphonato}oxy)phosphonateCHEMBL2364562 |
Ortholog Group (OrthoMCL): OG6_100455
Most Similar Human Ortholog: P49448
TM-align score: 0.77 | RMSD: 2.03
Seq Identity: 0.29 | Length: 415 / 510
All Human Orthologs (OrthoMCL):
| Gene ID | Description |
|---|---|
| ENSG00000148672 | glutamate dehydrogenase 1 |
| ENSG00000182890 | glutamate dehydrogenase 2 |
| ENSG00000288118 | glutamate dehydrogenase 2 |
Protein Length: 510 | Molecular Weight (kDa): 57.343
UniProt ID(s): Q8ILF7
PDB ID(s): 3R3J
Isoelectric Point: 9.2
Protein Domain Annotations:
| Source | Family ID | Description |
|---|---|---|
| InterPro | IPR006095 | Glutamate/phenylalanine/leucine/valine dehydrogenase |
| InterPro | IPR006096 | Glutamate/phenylalanine/leucine/valine dehydrogenase, C-terminal |
| InterPro | IPR006097 | Glutamate/phenylalanine/leucine/valine dehydrogenase, dimerisation domain |
| InterPro | IPR014362 | Glutamate dehydrogenase |
| InterPro | IPR033524 | Leu/Phe/Val dehydrogenases active site |
| InterPro | IPR033922 | NAD(P) binding domain of glutamate dehydrogenase |
| InterPro | IPR036291 | NAD(P)-binding domain superfamily |
| PFam | PF00208 | Glutamate/phenylalanine/leucine/valine dehydrogenase, C-terminal |
| PFam | PF02812 | Glutamate/phenylalanine/leucine/valine dehydrogenase, dimerisation domain |
| Superfamily | SSF51735 | NAD(P)-binding domain superfamily |
| Superfamily | SSF53223 |
MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:
Homozygous genotype calls only
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 4 | 15 | 13 | 36 |
| disruptive | 4 | 29 | 18 | 50 |
| missense | 4 | 29 | 18 | 43 |
Any inclusion in genotype call
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 5 | 45 | 19 | 41 |
| disruptive | 8 | 77 | 35 | 95 |
| missense | 8 | 70 | 32 | 69 |
PlasmoDB Total SNPs: 107
Non-coding: 91 | Synonymous: 11 | Nonsynonymous: 5 | Stop Codon: 0
| PMID | Title | Authors | DOI/Link |
|---|---|---|---|
| 12368864 | Genome sequence of the human malaria parasite Plasmodium falciparum. | Gardner MJ, Hall N, ..., Barrell B | 10.1038/nature01097 |
| 22342964 | Biochemical and structural characterization of Plasmodium falciparum glutamatedehydrogenase 2. | Zocher K, Fritz-Wolf K, ..., Becker K | 10.1016/j.molbiopara.2012.01.007 |
| 27128092 | Interrogating the Plasmodium Sporozoite Surface: Identification ofSurface-Exposed Proteins and Demonstration of Glycosylation on CSP and TRAP byMass Spectrometry-Based Proteomics. | Swearingen KE, Lindner SE, ..., Sinnis P | 10.1371/journal.ppat.1005606 |
| 30212465 | Integrative proteomics and bioinformatic prediction enable a high-confidenceapicoplast proteome in malaria parasites. | Boucher MJ, Ghosh S, ..., Yeh E | 10.1371/journal.pbio.2005895 |
| 38254622 | Structural Evolution of Primate Glutamate Dehydrogenase 2 as Revealed by InSilico Predictions and Experimentally Determined Structures | Litso I, Plaitakis A, ..., Zaganas I | 10.3390/biom14010022 |
| 34653675 | Carbon starvation-induced synthesis of GDH2 and PEPCK is essential for thesurvival of Pichia pastoris | Dey T, Rangarajan PN | 10.1016/j.bbrc.2021.10.015 |
| 38178569 | Diverse mechanisms control amino acid-dependent environmental alkalization byCandida albicans | Silao FGS, Valeriano VD, ..., Ljungdahl PO | 10.1111/mmi.15216 |
| 38154715 | The chromatin remodeler Ino80 regulates yeast stress tolerance and cellmetabolism through modulating nitrogen catabolite repression | Yuan B, Wang WB, ..., Zhao XQ | 10.1016/j.ijbiomac.2023.129041 |
| 36098787 | Rapamycin enhanced the production of 2-phenylethanol during whole-cellbioconversion by yeast | Xia H, Shangguan L, ..., Chen X | 10.1007/s00253-022-12169-6 |