Genome location: Pf3D7_14_v3:1,216,478..1,217,316(-)
Genome classification: Core
Product Description: cytochrome c oxidase subunit 2B, putative
SignalP Peptide: N/A
# Transmembrane Domains: 0
EC Numbers: 1.9.3.1 (Transferred entry: 7.1.1.9)
Curated GO (PlasmoDB):
| Type | GO Term | Name |
|---|---|---|
| Component | GO:0005751 | mitochondrial respiratory chain complex IV |
| Component | GO:0005739 | mitochondrion |
| Function | GO:0004129 | cytochrome-c oxidase activity |
| Process | GO:0042773 | ATP synthesis coupled electron transport |
Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):
| Stage | LR class | MCA mean | MCA prop. zeros |
|---|---|---|---|
| Sporozoite | possibly expressed | N/A | N/A |
| Ring | not expressed | 0.02 | 0.99 |
| Trophozoite | expressed | 0.15 | 0.90 |
| Schizont | expressed | 0.16 | 0.89 |
| Gametocyte | expressed | 0.28 | 0.83 |
More info:
Old (Pf3D7v3) Gene ID: PF3D7_1430900
Resistome Missense Mutations: None
Resistome Compounds with Missense Mutations: None
Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)
Zhang Phenotype: Non - Mutable in CDS
MIS: 0.208 | MFS: -2.528 | #Insertions: 0
PlasmoGEM Phenotype: Essential (Pb ortholog: PBANKA_1013800)
RMgmDB ABS Phenotype: N/A
More info: PhenoPlasm Link
AlphaFill Uniprot ID: Q8I6V2
"Best" AlphaFill ligand hit: ANP (phosphoaminophosphonic acid-adenylate ester, Local RMSD=7.68) with 7EIV (Global RMSD=20.50)
BRENDA EC Inhibitors:
| EC # | Name | EC Inhibitors |
|---|---|---|
| 1.9.3.1 | cytochrome-c oxidase | No BRENDA inhibitors |
Orthology to BindingDB Entries:
| UniProt | Protein Name | Species | Homology Source | BindingDB Ligands |
|---|---|---|---|---|
| P00403 | Cytochrome c oxidase subunit 2 | Homo sapiens | HOGENOM, OrthoMCL BLAST | ValdecoxibCHEMBL254418CelecoxibRofecoxib... |
| O78750 | Cytochrome c oxidase subunit 2 | Ovis aries | HOGENOM, OrthoDB, OrthoMCL BLAST | CHEMBL1254699CHEMBL1254700CHEMBL1254784CHEMBL1254785... |
Ortholog Group (OrthoMCL): OG6_103253
Most Similar Human Ortholog: P00403
TM-align score: 0.66 | RMSD: 1.82
Seq Identity: 0.43 | Length: 122 / 172
All Human Orthologs (OrthoMCL):
| Gene ID | Description |
|---|---|
| ENSG00000198712 | mitochondrially encoded cytochrome c oxidase II |
Protein Length: 172 | Molecular Weight (kDa): 19.786
UniProt ID(s): Q8I6V2
PDB ID(s): None
Isoelectric Point: 6.64
Protein Domain Annotations:
| Source | Family ID | Description |
|---|---|---|
| InterPro | IPR001505 | Copper centre Cu(A) |
| InterPro | IPR002429 | Cytochrome c oxidase subunit II-like C-terminal |
| InterPro | IPR008972 | Cupredoxin |
| PFam | PF00116 | Cytochrome c oxidase subunit II-like C-terminal |
| Superfamily | SSF49503 | Cupredoxin |
MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:
Homozygous genotype calls only
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 4 | 8 | 3 | 12 |
| disruptive | 2 | 5 | 6 | 9 |
| missense | 2 | 5 | 6 | 9 |
Any inclusion in genotype call
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 4 | 23 | 6 | 2 |
| disruptive | 2 | 21 | 10 | 40 |
| missense | 2 | 18 | 8 | 24 |
PlasmoDB Total SNPs: 40
Non-coding: 26 | Synonymous: 10 | Nonsynonymous: 3 | Stop Codon: 1
| PMID | Title | Authors | DOI/Link |
|---|---|---|---|
| 12368864 | Genome sequence of the human malaria parasite Plasmodium falciparum. | Gardner MJ, Hall N, ..., Barrell B | 10.1038/nature01097 |
| 18036550 | The polypeptides COX2A and COX2B are essential components of the mitochondrialcytochrome c oxidase of Toxoplasma gondii. | Morales-Sainz L, Escobar-Ramirez A, ..., Gonzalez-Halphen D | 10.1016/j.bbabio.2007.10.013 |
| 30204084 | Elucidating the mitochondrial proteome of Toxoplasma gondii reveals the presenceof a divergent cytochrome c oxidase | Seidi A, Muellner-Wong LS, ..., van Dooren GG | 10.7554/eLife.38131 |
| 34494883 | A Prioritized and Validated Resource of Mitochondrial Proteins in PlasmodiumIdentifies Unique Biology. | van Esveld SL, Meerstein-Kessel L, ..., Huynen MA | 10.1128/mSphere.00614-21 |
| 28359330 | Evolutionarily recent, insertional fission of mitochondrial cox2 into complementary genes in bilaterian Metazoa | Szafranski P | 10.1186/s12864-017-3626-5 |
| 29043530 | Cox2A/Cox2B subunit interaction in Polytomella sp. cytochrome c oxidase: role ofthe Cox2B subunit extension | Jimenez-Suarez A, Vazquez-Acevedo M, Miranda-Astudillo H, Gonzalez-Halphen D | 10.1007/s10863-017-9728-6 |
| 22425815 | In Polytomella sp. mitochondria, biogenesis of the heterodimeric COX2 subunit ofcytochrome c oxidase requires two different import pathways | Jimenez-Suarez A, Vazquez-Acevedo M, ..., Gonzalez-Halphen D | 10.1016/j.bbabio.2012.02.038 |