Genome location: Pf3D7_14_v3:1,230,044..1,234,486(+)
Genome classification: Core
Product Description: large subunit GTPase 1, putative
SignalP Peptide: N/A
# Transmembrane Domains: 0
EC Numbers: 3.6.1.- (In phosphorus-containing anhydrides.);3.6.5.1 (Heterotrimeric G-protein GTPase)
Curated GO (PlasmoDB):
| Type | GO Term | Name |
|---|---|---|
| Component | GO:0005829 | cytosol |
| Component | GO:0022625 | cytosolic large ribosomal subunit |
| Function | GO:0003924 | GTPase activity |
| Process | GO:0000027 | ribosomal large subunit assembly |
Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):
| Stage | LR class | MCA mean | MCA prop. zeros |
|---|---|---|---|
| Sporozoite | not expressed | N/A | N/A |
| Ring | expressed | 0.90 | 0.60 |
| Trophozoite | expressed | 0.54 | 0.71 |
| Schizont | possibly expressed | 0.13 | 0.92 |
| Gametocyte | expressed | 0.48 | 0.73 |
More info:
Old (Pf3D7v3) Gene ID: PF3D7_1431300
Resistome Missense Mutations: None
Resistome Compounds with Missense Mutations: None
Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)
Zhang Phenotype: Mutable in CDS
MIS: 0.783 | MFS: -0.127 | #Insertions: 1
PlasmoGEM Phenotype: Essential (Pb ortholog: PBANKA_1013500)
RMgmDB ABS Phenotype: N/A
More info: PhenoPlasm Link
AlphaFill Uniprot ID: Q8ILF2
"Best" AlphaFill ligand hit: GNP (phosphoaminophosphonic acid-guanylate ester, Local RMSD=0.17) with 3A1U (Global RMSD=13.28)
BRENDA EC Inhibitors:
| EC # | Name | EC Inhibitors |
|---|---|---|
| 3.6.1.- | Ex: CDP-glycerol diphosphatase | ATPADPGTPCTPUTPKClKCNITPNaFTTPAMPNMN... |
| 3.6.5.1 | heterotrimeric G-protein GTPase | GDPGpp(NH)pYM-254890phosducinpertussis toxinPasteurella multocida toxinLeu-Gly-Asn repeat-enriched proteinregulator of G protein signaling proteinbetagamma subunit of the hetreotrimeric G protein |
No evidence of orthology to BindingDB entries
Ortholog Group (OrthoMCL): OG6_101986
Most Similar Human Ortholog: Q9H089
TM-align score: 0.56 | RMSD: 4.31
Seq Identity: 0.31 | Length: 417 / 833
All Human Orthologs (OrthoMCL):
| Gene ID | Description |
|---|---|
| ENSG00000041802 | large 60S subunit nuclear export GTPase 1 |
MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:
Homozygous genotype calls only
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 12 | 31 | 18 | 51 |
| disruptive | 173 | 141 | 79 | 128 |
| missense | 61 | 64 | 55 | 80 |
Any inclusion in genotype call
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 18 | 66 | 21 | 57 |
| disruptive | 205 | 262 | 109 | 223 |
| missense | 64 | 157 | 68 | 132 |
PlasmoDB Total SNPs: 340
Non-coding: 132 | Synonymous: 81 | Nonsynonymous: 125 | Stop Codon: 2
Protein Length: 833 | Molecular Weight (kDa): 98.316
UniProt ID(s): Q8ILF2
PDB ID(s): None
Isoelectric Point: 5.05
Protein Domain Annotations:
| Source | Family ID | Description |
|---|---|---|
| InterPro | IPR006073 | GTP binding domain |
| InterPro | IPR027417 | P-loop containing nucleoside triphosphate hydrolase |
| InterPro | IPR043358 | Ras GTPase GNL1-like |
| PFam | PF01926 | GTP binding domain |
| Superfamily | SSF52540 | P-loop containing nucleoside triphosphate hydrolase |
| PMID | Title | Authors | DOI/Link |
|---|---|---|---|
| 12368864 | Genome sequence of the human malaria parasite Plasmodium falciparum. | Gardner MJ, Hall N, ..., Barrell B | 10.1038/nature01097 |
| 16267556 | A protein interaction network of the malaria parasite Plasmodium falciparum. | LaCount DJ, Vignali M, ..., Hughes RE | 10.1038/nature04104 |
| 33361368 | Acquired Resistance to Severe Ethanol Stress in Saccharomyces cerevisiae ProteinQuality Control | Yoshida M, Kato S, Fukuda S, Izawa S | 10.1128/AEM.02353-20 |