Genome location: Pf3D7_14_v3:1,238,794..1,242,545(-)
Genome classification: Core
Product Description: mitogen-activated protein kinase 1
SignalP Peptide: N/A
# Transmembrane Domains: 0
EC Numbers: 2.7.11.24 (Mitogen-activated protein kinase)
Curated GO (PlasmoDB):
| Type | GO Term | Name |
|---|---|---|
| Component | GO:0005737 | cytoplasm |
| Component | GO:0005634 | nucleus |
| Function | GO:0004707 | MAP kinase activity |
| Process | GO:0035556 | intracellular signal transduction |
| Process | GO:0006468 | protein phosphorylation |
| Process | GO:0010468 | regulation of gene expression |
Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):
| Stage | LR class | MCA mean | MCA prop. zeros |
|---|---|---|---|
| Sporozoite | expressed | N/A | N/A |
| Ring | possibly expressed | 0.13 | 0.94 |
| Trophozoite | expressed | 0.62 | 0.64 |
| Schizont | expressed | 0.38 | 0.77 |
| Gametocyte | expressed | 0.72 | 0.62 |
More info:
Old (Pf3D7v3) Gene ID: PF3D7_1431500
Resistome Missense Mutations: None
Resistome Compounds with Missense Mutations: None
Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)
Zhang Phenotype: Mutable in CDS
MIS: 1 | MFS: -2.499 | #Insertions: 1
PlasmoGEM Phenotype: Dispensable (Pb ortholog: PBANKA_1013300)
RMgmDB ABS Phenotype: Not different from wild type (Pb ortholog: PBANKA_1013300)
Modification: Disrupted | RMgm-4551
More info: PhenoPlasm Link
AlphaFill Uniprot ID: Q8ILF0
"Best" AlphaFill ligand hit: I46 (2-fluoro-4-[4-(4-fluorophenyl)-1h-pyrazol-3-yl]pyridine, Local RMSD=1.00) with 3K3I (Global RMSD=2.53)
BRENDA EC Inhibitors:
| EC # | Name | EC Inhibitors |
|---|---|---|
| 2.7.11.24 | mitogen-activated protein kinase | ADPH89MKK4MKP3MG132U0126SB202VX745siRNAML3403SR3451SR3582... |
Orthology to BindingDB Entries:
| UniProt | Protein Name | Species | Homology Source | BindingDB Ligands |
|---|---|---|---|---|
| Q8TD08 | Mitogen-activated protein kinase 15 | Homo sapiens | OrthoDB, OrthoMCL BLAST | CHEMBL1234833Staurosporine, 8CHEMBL2430323CHEMBL4075720... |
Ortholog Group (OrthoMCL): OG6_102994
Most Similar Human Ortholog: A0A1C7CYZ1
TM-align score: 0.87 | RMSD: 1.66
Seq Identity: 0.49 | Length: 256 / 914
All Human Orthologs (OrthoMCL):
| Gene ID | Description |
|---|---|
| ENSG00000181085 | mitogen-activated protein kinase 15 |
| ENSG00000274205 | mitogen-activated protein kinase 15 |
Protein Length: 914 | Molecular Weight (kDa): 107.277
UniProt ID(s): Q25758, Q8ILF0, Q94656
PDB ID(s): None
Isoelectric Point: 9.88
Protein Domain Annotations:
| Source | Family ID | Description |
|---|---|---|
| InterPro | IPR000719 | Protein kinase domain |
| InterPro | IPR003527 | Mitogen-activated protein (MAP) kinase, conserved site |
| InterPro | IPR008271 | Serine/threonine-protein kinase, active site |
| InterPro | IPR011009 | Protein kinase-like domain superfamily |
| InterPro | IPR017441 | Protein kinase, ATP binding site |
| PFam | PF00069 | Protein kinase domain |
| Superfamily | SSF56112 | Protein kinase-like domain superfamily |
| Superfamily | SSF69349 |
MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:
Homozygous genotype calls only
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 4 | 44 | 34 | 41 |
| disruptive | 24 | 77 | 45 | 137 |
| missense | 12 | 65 | 39 | 111 |
Any inclusion in genotype call
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 10 | 80 | 38 | 57 |
| disruptive | 43 | 174 | 83 | 195 |
| missense | 21 | 136 | 70 | 123 |
PlasmoDB Total SNPs: 178
Non-coding: 72 | Synonymous: 74 | Nonsynonymous: 32 | Stop Codon: 0
| PMID | Title | Authors | DOI/Link |
|---|---|---|---|
| 9004228 | Characterization of a mitogen-activated protein (MAP) kinase from Plasmodiumfalciparum. | Graeser R, Kury P, Franklin RM, Kappes B | 10.1046/j.1365-2958.1997.2071571.x |
| 12368864 | Genome sequence of the human malaria parasite Plasmodium falciparum. | Gardner MJ, Hall N, ..., Barrell B | 10.1038/nature01097 |
| 16267556 | A protein interaction network of the malaria parasite Plasmodium falciparum. | LaCount DJ, Vignali M, ..., Hughes RE | 10.1038/nature04104 |
| 23544094 | Plasmodium berghei MAPK1 displays differential and dynamic subcellularlocalizations during liver stage development. | Wierk JK, Langbehn A, ..., Deschermeier C | 10.1371/journal.pone.0059755 |