Genome location: Pf3D7_14_v3:1,242,924..1,245,905(+)
Genome classification: Core
Product Description: succinate--CoA ligase [ADP-forming] subunit beta
SignalP Peptide: N/A
# Transmembrane Domains: 0
EC Numbers: 6.2.1.5 (Succinate--CoA ligase (ADP-forming))
Curated GO (PlasmoDB):
| Type | GO Term | Name |
|---|---|---|
| Component | GO:0005759 | mitochondrial matrix |
| Component | GO:0005739 | mitochondrion |
| Component | GO:0042709 | succinate-CoA ligase complex |
| Function | GO:0004775 | succinate-CoA ligase (ADP-forming) activity |
| Function | GO:0004776 | succinate-CoA ligase (GDP-forming) activity |
| Process | GO:0006104 | succinyl-CoA metabolic process |
| Process | GO:0006099 | tricarboxylic acid cycle |
Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):
| Stage | LR class | MCA mean | MCA prop. zeros |
|---|---|---|---|
| Sporozoite | expressed | N/A | N/A |
| Ring | not expressed | 0.02 | 0.99 |
| Trophozoite | expressed | 0.19 | 0.87 |
| Schizont | expressed | 0.10 | 0.92 |
| Gametocyte | expressed | 0.16 | 0.90 |
More info:
Old (Pf3D7v3) Gene ID: PF3D7_1431600
Resistome Missense Mutations: None
Resistome Compounds with Missense Mutations: None
Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)
Zhang Phenotype: Mutable in CDS
MIS: 1 | MFS: -0.263 | #Insertions: 5
PlasmoGEM Phenotype: N/A
RMgmDB ABS Phenotype: N/A
More info: PhenoPlasm Link
AlphaFill Uniprot ID: Q8ILE9
"Best" AlphaFill ligand hit: COA (coenzyme a, Local RMSD=0.21) with 2NU6 (Global RMSD=1.71)
BRENDA EC Inhibitors:
| EC # | Name | EC Inhibitors |
|---|---|---|
| 6.2.1.5 | succinate-CoA ligase (ADP-forming) | BRENDA SOAP query unsuccessful |
No evidence of orthology to BindingDB entries
Ortholog Group (OrthoMCL): OG6_100409
Most Similar Human Ortholog: A0A2R8YDQ9
TM-align score: 0.94 | RMSD: 1.89
Seq Identity: 0.46 | Length: 402 / 462
All Human Orthologs (OrthoMCL):
| Gene ID | Description |
|---|---|
| ENSG00000136143 | succinate-CoA ligase ADP-forming subunit beta |
| ENSG00000172340 | succinate-CoA ligase GDP-forming subunit beta |
MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:
Homozygous genotype calls only
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 2 | 8 | 16 | 33 |
| disruptive | 1 | 17 | 12 | 40 |
| missense | 1 | 17 | 11 | 38 |
Any inclusion in genotype call
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 3 | 44 | 15 | 18 |
| disruptive | 5 | 47 | 36 | 76 |
| missense | 3 | 35 | 29 | 58 |
PlasmoDB Total SNPs: 112
Non-coding: 101 | Synonymous: 6 | Nonsynonymous: 5 | Stop Codon: 0
Protein Length: 462 | Molecular Weight (kDa): 52.17
UniProt ID(s): Q8ILE9
PDB ID(s): None
Isoelectric Point: 7.86
Protein Domain Annotations:
| Source | Family ID | Description |
|---|---|---|
| InterPro | IPR005809 | Succinate--CoA synthetase, beta subunit |
| InterPro | IPR005811 | ATP-citrate lyase/succinyl-CoA ligase |
| InterPro | IPR013650 | ATP-grasp fold, succinyl-CoA synthetase-type |
| InterPro | IPR016102 | Succinyl-CoA synthetase-like |
| PFam | PF00549 | ATP-citrate lyase/succinyl-CoA ligase |
| PFam | PF08442 | ATP-grasp fold, succinyl-CoA synthetase-type |
| Superfamily | SSF52210 | Succinyl-CoA synthetase-like |
| Superfamily | SSF56059 |
| PMID | Title | Authors | DOI/Link |
|---|---|---|---|
| 12368864 | Genome sequence of the human malaria parasite Plasmodium falciparum. | Gardner MJ, Hall N, ..., Barrell B | 10.1038/nature01097 |
| 33174373 | The nucleotide specificity of succinyl-CoA synthetase of Plasmodium falciparum isnot determined by charged gatekeeper residues alone. | Vashisht K, Singh P, ..., Pandey KC | 10.1002/2211-5463.13034 |
| 34494883 | A Prioritized and Validated Resource of Mitochondrial Proteins in PlasmodiumIdentifies Unique Biology. | van Esveld SL, Meerstein-Kessel L, ..., Huynen MA | 10.1128/mSphere.00614-21 |