Genome location: Pf3D7_14_v3:1,249,311..1,252,547(+)
Genome classification: Core
Product Description: apyrase, putative
SignalP Peptide: N/A
# Transmembrane Domains: 2
EC Numbers: 3.6.1.5 (Apyrase)
Curated GO (PlasmoDB):
| Type | GO Term | Name |
|---|---|---|
| Component | GO:0005783 | endoplasmic reticulum |
| Component | GO:0020020 | food vacuole |
Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):
| Stage | LR class | MCA mean | MCA prop. zeros |
|---|---|---|---|
| Sporozoite | not expressed | N/A | N/A |
| Ring | possibly expressed | 0.14 | 0.93 |
| Trophozoite | expressed | 0.33 | 0.80 |
| Schizont | possibly expressed | 0.03 | 0.98 |
| Gametocyte | not expressed | 0.19 | 0.89 |
More info:
Old (Pf3D7v3) Gene ID: PF3D7_1431800
Resistome Missense Mutations: None
Resistome Compounds with Missense Mutations: None
Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)
Zhang Phenotype: Mutable in CDS
MIS: 1 | MFS: -2.209 | #Insertions: 4
PlasmoGEM Phenotype: N/A
RMgmDB ABS Phenotype: N/A
More info: PhenoPlasm Link
AlphaFill Uniprot ID: Q8ILE7
"Best" AlphaFill ligand hit: AMP (adenosine monophosphate, Local RMSD=0.06) with 5U7V (Global RMSD=3.93)
BRENDA EC Inhibitors:
| EC # | Name | EC Inhibitors |
|---|---|---|
| 3.6.1.5 | apyrase | BRENDA SOAP query unsuccessful |
No evidence of orthology to BindingDB entries
Ortholog Group (OrthoMCL): OG6_101124
Most Similar Human Ortholog: P49961
TM-align score: 0.74 | RMSD: 3.66
Seq Identity: 0.25 | Length: 429 / 874
All Human Orthologs (OrthoMCL):
| Gene ID | Description |
|---|---|
| ENSG00000138185 | ectonucleoside triphosphate diphosphohydrolase 1 |
| ENSG00000197217 | ectonucleoside triphosphate diphosphohydrolase 4 |
| ENSG00000198018 | ectonucleoside triphosphate diphosphohydrolase 7 |
MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:
Homozygous genotype calls only
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 7 | 45 | 25 | 46 |
| disruptive | 18 | 110 | 63 | 165 |
| missense | 16 | 100 | 62 | 143 |
Any inclusion in genotype call
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 14 | 75 | 38 | 46 |
| disruptive | 46 | 214 | 117 | 245 |
| missense | 36 | 189 | 101 | 182 |
PlasmoDB Total SNPs: 106
Non-coding: 37 | Synonymous: 37 | Nonsynonymous: 32 | Stop Codon: 0
Protein Length: 874 | Molecular Weight (kDa): 105.033
UniProt ID(s): Q8ILE7
PDB ID(s): None
Isoelectric Point: 9.49
Protein Domain Annotations:
| Source | Family ID | Description |
|---|---|---|
| InterPro | IPR000407 | Nucleoside phosphatase GDA1/CD39 |
| PFam | PF01150 | Nucleoside phosphatase GDA1/CD39 |
| Superfamily | N/A | N/A |
| PMID | Title | Authors | DOI/Link |
|---|---|---|---|
| 12368864 | Genome sequence of the human malaria parasite Plasmodium falciparum. | Gardner MJ, Hall N, ..., Barrell B | 10.1038/nature01097 |
| 28285440 | Plasmodium falciparum GFP-E-NTPDase expression at the intraerythrocytic stagesand its inhibition blocks the development of the human malaria parasite. | Borges-Pereira L, Meissner KA, Wrenger C, Garcia CRS | 10.1007/s11302-017-9557-4 |
| 32851963 | Evidences of G Coupled-Protein Receptor (GPCR) Signaling in the human MalariaParasite Plasmodium falciparum for Sensing its Microenvironment and the Role ofPurinergic Signaling in Malaria Parasites | Pereira PHS, Borges-Pereira L, Garcia CRS | 10.2174/1568026620666200826122716 |
| 37975950 | Synthesis of new non-natural L-glycosidic flavonoid derivatives and theirevaluation as inhibitors of Trypanosoma cruzi ecto-nucleoside triphosphatediphosphohydrolase 1 (TcNTPDase1) | Ribeiro IC, de Moraes JVB, ..., Fietto JLR | 10.1007/s11302-023-09974-7 |