PF3D7_1432500

Genome location: Pf3D7_14_v3:1,283,342..1,285,383(+)

Genome classification: Core

Function and Localization

Product Description: RNA methyltransferase, putative

SignalP Peptide: N/A

# Transmembrane Domains: 0

EC Numbers: 2.1.1.34 (tRNA (guanosine(18)-2'-O)-methyltransferase)

Curated GO (PlasmoDB): None

Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):

Stage	LR class	MCA mean	MCA prop. zeros
Sporozoite	possibly expressed	N/A	N/A
Ring	expressed	0.33	0.84
Trophozoite	expressed	0.39	0.77
Schizont	possibly expressed	0.09	0.94
Gametocyte	expressed	0.59	0.66

More info:

Resistome Mutations

Old (Pf3D7v3) Gene ID: PF3D7_1432500

Resistome Missense Mutations: None

Resistome Compounds with Missense Mutations: None

Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)

Essentiality (ABS)

Zhang Phenotype: Non - Mutable in CDS

MIS: 0.135 | MFS: -3.165 | #Insertions: 0

PlasmoGEM Phenotype: Slow (Pb ortholog: PBANKA_1012400)

Relative Growth Rate: 0.48 ± 0.09
Confidence: 6.27

RMgmDB ABS Phenotype: Different from wild type (Pb ortholog: PBANKA_1012400)

Modification: Disrupted | RMgm-2770

More info: PhenoPlasm Link

Binding Evidence

AlphaFill Uniprot ID: Q8ILD7

"Best" AlphaFill ligand hit: PGO (s-1,2-propanediol, Local RMSD=0.29) with 4RG1 (Global RMSD=4.37)

BRENDA EC Inhibitors:

EC #	Name	EC Inhibitors
2.1.1.34	tRNA (guanosine18-2'-O)-methyltransferase	PolyamineDELTAD-armS-adenosyl-L-homocysteine

No evidence of orthology to BindingDB entries

Orthology Information

Ortholog Group (OrthoMCL): OG6_102561

Most Similar Human Ortholog: Q5T280

TM-align score: 0.72 | RMSD: 3.14

Seq Identity: 0.35 | Length: 299 / 444

All Human Orthologs (OrthoMCL):

Gene ID	Description
ENSG00000198917	SPOUT domain containing methyltransferase 1

Genetic Variation

MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:

Homozygous genotype calls only

variant type	common	rare	doubleton	singleton
synonymous	6	24	12	23
disruptive	3	32	25	64
missense	3	31	24	56

Any inclusion in genotype call

variant type	common	rare	doubleton	singleton
synonymous	9	39	22	30
disruptive	11	88	52	121
missense	6	75	43	82

PlasmoDB Total SNPs: 84

Non-coding: 53 | Synonymous: 22 | Nonsynonymous: 8 | Stop Codon: 1

Protein Information

Protein Length: 444 | Molecular Weight (kDa): 51.847

UniProt ID(s): Q8ILD7

PDB ID(s): None

Isoelectric Point: 9.61

Protein Domain Annotations:

Source	Family ID	Description
InterPro	IPR003750	Putative RNA methyltransferase
InterPro	IPR012340	Nucleic acid-binding, OB-fold
InterPro	IPR029028	Alpha/beta knot methyltransferases
PFam	PF02598	Putative RNA methyltransferase
Superfamily	SSF50249	Nucleic acid-binding, OB-fold
Superfamily	SSF75217	Alpha/beta knot methyltransferases