PF3D7_1432600

Genome location: Pf3D7_14_v3:1,285,502..1,289,263(-)

Genome classification: Core

Function and Localization

Product Description: DNA-binding protein, putative

SignalP Peptide: N/A

# Transmembrane Domains: 0

EC Numbers: None

Curated GO (PlasmoDB):

Type	GO Term	Name
Component	GO:0071942	XPC complex
Component	GO:0005737	cytoplasm
Component	GO:0000111	nucleotide-excision repair factor 2 complex
Function	GO:0003684	damaged DNA binding
Function	GO:0003697	single-stranded DNA binding
Process	GO:0006298	mismatch repair
Process	GO:0006289	nucleotide-excision repair

Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):

Stage	LR class	MCA mean	MCA prop. zeros
Sporozoite	not expressed	N/A	N/A
Ring	not expressed	0.23	0.89
Trophozoite	possibly expressed	0.60	0.66
Schizont	expressed	0.15	0.91
Gametocyte	possibly expressed	0.47	0.74

More info:

Resistome Mutations

Old (Pf3D7v3) Gene ID: PF3D7_1432600

Resistome Missense Mutations: None

Resistome Compounds with Missense Mutations: None

Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)

Essentiality (ABS)

Zhang Phenotype: Mutable in CDS

MIS: 1 | MFS: -1.503 | #Insertions: 4

PlasmoGEM Phenotype: Slow (Pb ortholog: PBANKA_1012300)

Relative Growth Rate: 0.77 ± 0.17
Confidence: 4.87

RMgmDB ABS Phenotype: Different from wild type (Pb ortholog: PBANKA_1012300)

Modification: Disrupted | RMgm-2769

More info: PhenoPlasm Link

Binding Evidence

AlphaFill Uniprot ID: Q8ILD6

"Best" AlphaFill ligand hit: AMP (adenosine monophosphate, Local RMSD=1.43) with 6JWQ (Global RMSD=43.45)

No associated EC numbers

No evidence of orthology to BindingDB entries

Orthology Information

Ortholog Group (OrthoMCL): OG6_532401

No human ortholog(s)

Genetic Variation

MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:

Homozygous genotype calls only

variant type	common	rare	doubleton	singleton
synonymous	11	51	35	65
disruptive	19	122	94	173
missense	16	118	88	160

Any inclusion in genotype call

variant type	common	rare	doubleton	singleton
synonymous	17	108	47	65
disruptive	54	276	140	230
missense	33	246	129	201

PlasmoDB Total SNPs: 79

Non-coding: 7 | Synonymous: 56 | Nonsynonymous: 16 | Stop Codon: 0

Protein Information

Protein Length: 1195 | Molecular Weight (kDa): 143.996

UniProt ID(s): Q8ILD6

PDB ID(s): None

Isoelectric Point: 9.21

Protein Domain Annotations:

Source	Family ID	Description
InterPro	IPR004583	DNA repair protein Rad4
InterPro	IPR018326	Rad4 beta-hairpin domain 1
InterPro	IPR038765	Papain-like cysteine peptidase superfamily
PFam	PF10403	Rad4 beta-hairpin domain 1
Superfamily	SSF54001	Papain-like cysteine peptidase superfamily

PMID	Title	Authors	DOI/Link
12368864	Genome sequence of the human malaria parasite Plasmodium falciparum.	Gardner MJ, Hall N, ..., Barrell B	10.1038/nature01097
16267556	A protein interaction network of the malaria parasite Plasmodium falciparum.	LaCount DJ, Vignali M, ..., Hughes RE	10.1038/nature04104