Product Description: protein-L-isoaspartate(D-aspartate) O-methyltransferase, putative
SignalP Peptide: N/A
# Transmembrane Domains: 0
EC Numbers: 2.1.1.77 (Protein-L-isoaspartate(D-aspartate) O-methyltransferase)
Curated GO (PlasmoDB):
| Type | GO Term | Name |
|---|---|---|
| Component | GO:0020036 | Maurer's cleft |
| Component | GO:0005737 | cytoplasm |
| Component | GO:0005634 | nucleus |
| Function | GO:0004719 | protein-L-isoaspartate (D-aspartate) O-methyltransferase activity |
| Process | GO:0030091 | protein repair |
Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):
| Stage | LR class | MCA mean | MCA prop. zeros |
|---|---|---|---|
| Sporozoite | expressed | N/A | N/A |
| Ring | expressed | 0.09 | 0.95 |
| Trophozoite | expressed | 0.75 | 0.59 |
| Schizont | expressed | 0.29 | 0.82 |
| Gametocyte | expressed | 0.87 | 0.54 |
More info:
Old (Pf3D7v3) Gene ID: PF3D7_1432700
Resistome Missense Mutations: None
Resistome Compounds with Missense Mutations: None
Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)
Zhang Phenotype: Mutable in CDS
MIS: 0.992 | MFS: -0.826 | #Insertions: 1
PlasmoGEM Phenotype: N/A
RMgmDB ABS Phenotype: N/A
More info: PhenoPlasm Link
AlphaFill Uniprot ID: A0A144A3E6
"Best" AlphaFill ligand hit: SAH (s-adenosyl-l-homocysteine, Local RMSD=0.35) with 2PBF (Global RMSD=0.49)
BRENDA EC Inhibitors:
| EC # | Name | EC Inhibitors |
|---|---|---|
| 2.1.1.77 | protein-L-isoaspartate(D-aspartate) O-methyltransferase | adenosinesinefunginadenosine dialdehydeS-adenosylhomocysteineS-adenosyl-L-homocysteine5'-deoxy-5'-methylthioadenosineNalpha-benzyloxycarbonyl-O-benzyl-L-Asp-Nepsilon-L-Lys-L-Tyr-L-Ala-amide |
No evidence of orthology to BindingDB entries
Ortholog Group (OrthoMCL): OG6_100731
Most Similar Human Ortholog: C9J0F2
TM-align score: 0.97 | RMSD: 0.64
Seq Identity: 0.47 | Length: 96 / 224
All Human Orthologs (OrthoMCL):
| Gene ID | Description |
|---|---|
| ENSG00000120265 | protein-L-isoaspartate (D-aspartate) O-methyltransferase |
MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:
Homozygous genotype calls only
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 2 | 14 | 5 | 18 |
| disruptive | 0 | 1 | 3 | 12 |
| missense | 0 | 1 | 2 | 9 |
Any inclusion in genotype call
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 5 | 24 | 7 | 16 |
| disruptive | 3 | 11 | 9 | 34 |
| missense | 0 | 8 | 7 | 25 |
PlasmoDB Total SNPs: 90
Non-coding: 82 | Synonymous: 8 | Nonsynonymous: 0 | Stop Codon: 0
Protein Length: 224 | Molecular Weight (kDa): 25.543
UniProt ID(s): A0A144A3E6
PDB ID(s): 2PBF
Isoelectric Point: 7.43
Protein Domain Annotations:
| Source | Family ID | Description |
|---|---|---|
| InterPro | IPR000682 | Protein-L-isoaspartate(D-aspartate) O-methyltransferase |
| InterPro | IPR029063 | S-adenosyl-L-methionine-dependent methyltransferase |
| PFam | PF01135 | N/A |
| Superfamily | SSF53335 | S-adenosyl-L-methionine-dependent methyltransferase |
| PMID | Title | Authors | DOI/Link |
|---|---|---|---|
| 12368864 | Genome sequence of the human malaria parasite Plasmodium falciparum. | Gardner MJ, Hall N, ..., Barrell B | 10.1038/nature01097 |
| 23181666 | Organellar proteomics reveals hundreds of novel nuclear proteins in the malariaparasite Plasmodium falciparum. | Oehring SC, Woodcroft BJ, ..., Voss TS | 10.1186/gb-2012-13-11-r108 |
| 31631203 | A network-based approach reveals novel invasion and Maurer's clefts-relatedproteins in Plasmodium falciparum. | Das D, Krishnan SR, Roy A, Bulusu G | 10.1039/c9mo00124g |