PF3D7_1433900

Genome location: Pf3D7_14_v3:1,359,700..1,366,130(+)

Genome classification: Core

Function and Localization

Product Description: protein kinase, putative

SignalP Peptide: N/A

# Transmembrane Domains: 6

EC Numbers: 2.7.11.1 (Non-specific serine/threonine protein kinase)

Curated GO (PlasmoDB):

Type	GO Term	Name
Component	GO:0005634	nucleus
Function	GO:0008353	RNA polymerase II CTD heptapeptide repeat kinase activity
Function	GO:0004693	cyclin-dependent protein serine/threonine kinase activity
Function	GO:0004672	protein kinase activity
Process	GO:0000086	G2/M transition of mitotic cell cycle
Process	GO:0007095	mitotic G2 DNA damage checkpoint signaling
Process	GO:0006468	protein phosphorylation

Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):

Stage	LR class	MCA mean	MCA prop. zeros
Sporozoite	not expressed	N/A	N/A
Ring	not expressed	0.07	0.97
Trophozoite	not expressed	0.43	0.75
Schizont	not expressed	0.34	0.79
Gametocyte	not expressed	0.80	0.59

More info:

Resistome Mutations

Old (Pf3D7v3) Gene ID: PF3D7_1433900

Resistome Missense Mutations: L1370I

Resistome Compounds with Missense Mutations: Desoxyepothilone B

Resistome # Samples with Disruptive Mutations: 1 (1 missense, 0 "interesting" missense)

Essentiality (ABS)

Zhang Phenotype: Mutable in CDS

MIS: 1 | MFS: -1.275 | #Insertions: 3

PlasmoGEM Phenotype: N/A

RMgmDB ABS Phenotype: N/A

More info: PhenoPlasm Link

Binding Evidence

AlphaFill Uniprot ID: Q8ILC4

"Best" AlphaFill ligand hit: GNP (phosphoaminophosphonic acid-guanylate ester, Local RMSD=7.55) with 4JR7 (Global RMSD=26.32)

BRENDA EC Inhibitors:

EC #	Name	EC Inhibitors
2.7.11.1	non-specific serine/threonine protein kinase	ATPKClDTTP53DRBEDTATBCAKN-93K252aCKI-7LAALSTAACS...

No evidence of orthology to BindingDB entries

Orthology Information

Ortholog Group (OrthoMCL): OG6_533573

No human ortholog(s)

Genetic Variation

MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:

Homozygous genotype calls only

variant type	common	rare	doubleton	singleton
synonymous	21	76	42	94
disruptive	75	296	156	314
missense	53	246	136	272

Any inclusion in genotype call

variant type	common	rare	doubleton	singleton
synonymous	35	147	61	110
disruptive	172	567	228	426
missense	99	455	192	309

PlasmoDB Total SNPs: 215

Non-coding: 14 | Synonymous: 112 | Nonsynonymous: 88 | Stop Codon: 1

Protein Information

Protein Length: 1518 | Molecular Weight (kDa): 186.672

UniProt ID(s): Q8ILC4

PDB ID(s): None

Isoelectric Point: 10.47

Protein Domain Annotations:

Source	Family ID	Description
InterPro	IPR000719	Protein kinase domain
InterPro	IPR011009	Protein kinase-like domain superfamily
InterPro	IPR017441	Protein kinase, ATP binding site
PFam	N/A	N/A
Superfamily	SSF56112	Protein kinase-like domain superfamily