PF3D7_1434100

Genome location: Pf3D7_14_v3:1,366,575..1,368,634(+)

Genome classification: Core

Function and Localization

Product Description: queuine tRNA-ribosyltransferase, putative

SignalP Peptide: N/A

# Transmembrane Domains: 0

EC Numbers: 2.4.2.29 (tRNA-guanine(34) transglycosylase)

Curated GO (PlasmoDB):

Type	GO Term	Name
Process	GO:0101030	tRNA-guanine transglycosylation

Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):

Stage	LR class	MCA mean	MCA prop. zeros
Sporozoite	not expressed	N/A	N/A
Ring	expressed	0.14	0.93
Trophozoite	expressed	0.14	0.92
Schizont	possibly expressed	0.03	0.98
Gametocyte	not expressed	0.05	0.97

More info:

Resistome Mutations

Old (Pf3D7v3) Gene ID: PF3D7_1434100

Resistome Missense Mutations: None

Resistome Compounds with Missense Mutations: None

Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)

Essentiality (ABS)

Zhang Phenotype: Mutable in CDS

MIS: 0.999 | MFS: -1.67 | #Insertions: 2

PlasmoGEM Phenotype: N/A

RMgmDB ABS Phenotype: N/A

More info: PhenoPlasm Link

Binding Evidence

AlphaFill Uniprot ID: Q8ILC2

"Best" AlphaFill ligand hit: No AlphaFill hits

BRENDA EC Inhibitors:

EC #	Name	EC Inhibitors
2.4.2.29	tRNA-guanosine34 preQ1 transglycosylase	ethylacetimidate4-aminophthalimidedimethylsuberimidate4-aminophthalhydrazide3,5-diaminophthalimide2,3-Dihydroxybenzoic acid2-aminopteridin-4(3H)-one3,5-diaminophthalhydrazide2-aminoquinazolin-4(3H)-onebenzimidazole-5-carboxylate2-methylamino-lin-benzoguanine2,6-diaminoquinazolin-4(3H)-one...

No evidence of orthology to BindingDB entries

Orthology Information

Ortholog Group (OrthoMCL): OG6_103114

Most Similar Human Ortholog: Q9H974

TM-align score: 0.82 | RMSD: 3.36

Seq Identity: 0.18 | Length: 378 / 407

All Human Orthologs (OrthoMCL):

Gene ID	Description
ENSG00000151576	queuine tRNA-ribosyltransferase accessory subunit 2

Genetic Variation

MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:

Homozygous genotype calls only

variant type	common	rare	doubleton	singleton
synonymous	5	25	8	33
disruptive	6	38	33	86
missense	6	38	32	74

Any inclusion in genotype call

variant type	common	rare	doubleton	singleton
synonymous	7	41	22	32
disruptive	16	101	49	106
missense	14	93	47	81

PlasmoDB Total SNPs: 67

Non-coding: 31 | Synonymous: 28 | Nonsynonymous: 8 | Stop Codon: 0

Protein Information

Protein Length: 407 | Molecular Weight (kDa): 47.829

UniProt ID(s): A0A144A3R0, P25408, Q8ILC2

PDB ID(s): None

Isoelectric Point: 6.09

Protein Domain Annotations:

Source	Family ID	Description
InterPro	IPR002616	tRNA-guanine(15) transglycosylase-like
InterPro	IPR036511	Queuine tRNA-ribosyltransferase-like
PFam	PF01702	tRNA-guanine(15) transglycosylase-like
Superfamily	SSF51713	Queuine tRNA-ribosyltransferase-like