PF3D7_1435100

Genome location: Pf3D7_14_v3:1,415,760..1,417,286(-)

Genome classification: Core

Function and Localization

Product Description: ribonuclease H2 subunit B, putative

SignalP Peptide: N/A

# Transmembrane Domains: 0

EC Numbers: None

Curated GO (PlasmoDB): None

Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):

Stage	LR class	MCA mean	MCA prop. zeros
Sporozoite	not expressed	N/A	N/A
Ring	not expressed	0.05	0.97
Trophozoite	expressed	0.53	0.69
Schizont	expressed	0.13	0.91
Gametocyte	possibly expressed	0.29	0.83

More info:

Resistome Mutations

Old (Pf3D7v3) Gene ID: PF3D7_1435100

Resistome Missense Mutations: None

Resistome Compounds with Missense Mutations: None

Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)

Essentiality (ABS)

Zhang Phenotype: Non - Mutable in CDS

MIS: 0.182 | MFS: -2.641 | #Insertions: 0

PlasmoGEM Phenotype: Slow (Pb ortholog: PBANKA_1009700)

Relative Growth Rate: 0.35 ± 0.06
Confidence: 7.00

RMgmDB ABS Phenotype: Different from wild type (Pb ortholog: PBANKA_1009700)

Modification: Disrupted | RMgm-2755

More info: PhenoPlasm Link

Binding Evidence

AlphaFill Uniprot ID: Q8ILB3

"Best" AlphaFill ligand hit: No AlphaFill hits

No associated EC numbers

No evidence of orthology to BindingDB entries

Orthology Information

Ortholog Group (OrthoMCL): OG6_156391

No human ortholog(s)

Genetic Variation

MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:

Homozygous genotype calls only

variant type	common	rare	doubleton	singleton
synonymous	3	12	12	28
disruptive	5	33	29	48
missense	5	33	28	48

Any inclusion in genotype call

variant type	common	rare	doubleton	singleton
synonymous	5	28	14	35
disruptive	12	78	42	83
missense	10	67	41	72

PlasmoDB Total SNPs: 47

Non-coding: 31 | Synonymous: 9 | Nonsynonymous: 7 | Stop Codon: 0

Protein Information

Protein Length: 359 | Molecular Weight (kDa): 43.426

UniProt ID(s): Q8ILB3

PDB ID(s): None

Isoelectric Point: 9.4

Protein Domain Annotations:

Source	Family ID	Description
InterPro	IPR019024	Ribonuclease H2 subunit B, wHTH domain
PFam	PF09468	Ribonuclease H2 subunit B, wHTH domain
Superfamily	N/A	N/A