PF3D7_1435300

Genome location: Pf3D7_14_v3:1,421,241..1,431,920(-)

Genome classification: Core

Function and Localization

Product Description: glutamate synthase [NADH], putative

SignalP Peptide: N/A

# Transmembrane Domains: 0

EC Numbers: 1.4.1.14 (Glutamate synthase (NADH))

Curated GO (PlasmoDB):

Type	GO Term	Name
Function	GO:0016040	glutamate synthase (NADH) activity
Function	GO:0015930	glutamate synthase activity
Function	GO:0016491	oxidoreductase activity
Process	GO:0019676	ammonia assimilation cycle
Process	GO:0006537	glutamate biosynthetic process

Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):

Stage	LR class	MCA mean	MCA prop. zeros
Sporozoite	expressed	N/A	N/A
Ring	not expressed	0.19	0.91
Trophozoite	expressed	0.52	0.72
Schizont	expressed	1.55	0.30
Gametocyte	expressed	0.52	0.71

More info:

Resistome Mutations

Old (Pf3D7v3) Gene ID: PF3D7_1435300

Resistome Missense Mutations: None

Resistome Compounds with Missense Mutations: None

Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)

Essentiality (ABS)

Zhang Phenotype: Mutable in CDS

MIS: 1 | MFS: -0.382 | #Insertions: 12

PlasmoGEM Phenotype: Slow (Pb ortholog: PBANKA_1009500)

Relative Growth Rate: 0.72 ± 0.19
Confidence: 4.67

RMgmDB ABS Phenotype: Not different from wild type (Pb ortholog: PBANKA_1009500)

Modification: Disrupted | RMgm-4064

More info: PhenoPlasm Link

Binding Evidence

AlphaFill Uniprot ID: N/A

"Best" AlphaFill ligand hit: No AlphaFill hits

BRENDA EC Inhibitors:

EC #	Name	EC Inhibitors
1.4.1.14	glutamate synthase (NADH)	BRENDA SOAP query unsuccessful

No evidence of orthology to BindingDB entries

Orthology Information

Ortholog Group (OrthoMCL): OG6_100905

No human ortholog(s)

Genetic Variation

MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:

Homozygous genotype calls only

variant type	common	rare	doubleton	singleton
synonymous	22	149	94	194
disruptive	88	219	131	300
missense	49	165	100	237

Any inclusion in genotype call

variant type	common	rare	doubleton	singleton
synonymous	56	336	133	193
disruptive	149	408	203	429
missense	74	307	167	332

PlasmoDB Total SNPs: 269

Non-coding: 90 | Synonymous: 107 | Nonsynonymous: 72 | Stop Codon: 0

Protein Information

Protein Length: 3106 | Molecular Weight (kDa): 353.344

UniProt ID(s): O61143, Q8ILB1

PDB ID(s): None

Isoelectric Point: 7.46

Protein Domain Annotations:

Source	Family ID	Description
InterPro	IPR002489	Glutamate synthase, alpha subunit, C-terminal
InterPro	IPR002932	Glutamate synthase domain
InterPro	IPR006005	Glutamate synthase, NADH/NADPH, small subunit 1
InterPro	IPR006982	Glutamate synthase, central-N
InterPro	IPR009051	Alpha-helical ferredoxin
InterPro	IPR017932	Glutamine amidotransferase type 2 domain
InterPro	IPR023753	FAD/NAD(P)-binding domain
InterPro	IPR028261	Dihydroprymidine dehydrogenase domain II
InterPro	IPR029055	Nucleophile aminohydrolases, N-terminal
InterPro	IPR036188	FAD/NAD(P)-binding domain superfamily
InterPro	IPR036485	Glutamate synthase, alpha subunit, C-terminal domain superfamily
PFam	PF00310	Glutamine amidotransferase type 2 domain
PFam	PF01493	Glutamate synthase, alpha subunit, C-terminal
PFam	PF01645	Glutamate synthase domain
PFam	PF04898	Glutamate synthase, central-N
PFam	PF07992	FAD/NAD(P)-binding domain
PFam	PF14691	Dihydroprymidine dehydrogenase domain II
Superfamily	SSF46548	Alpha-helical ferredoxin
Superfamily	SSF51395	FAD/NAD(P)-binding domain superfamily
Superfamily	SSF51905	Nucleophile aminohydrolases, N-terminal
Superfamily	SSF51971	Glutamate synthase, alpha subunit, C-terminal domain superfamily
Superfamily	SSF56235
Superfamily	SSF69336

PMID	Title	Authors	DOI/Link
12368864	Genome sequence of the human malaria parasite Plasmodium falciparum.	Gardner MJ, Hall N, ..., Barrell B	10.1038/nature01097
16267556	A protein interaction network of the malaria parasite Plasmodium falciparum.	LaCount DJ, Vignali M, ..., Hughes RE	10.1038/nature04104