Genome location: Pf3D7_14_v3:1,452,234..1,454,205(+)
Genome classification: Core
Product Description: GTPase Era, putative
SignalP Peptide: N/A
# Transmembrane Domains: 0
EC Numbers: 3.6.5.2 (Small monomeric GTPase)
Curated GO (PlasmoDB):
| Type | GO Term | Name |
|---|---|---|
| Component | GO:0005739 | mitochondrion |
| Function | GO:0019843 | rRNA binding |
| Function | GO:0043024 | ribosomal small subunit binding |
| Process | GO:0000028 | ribosomal small subunit assembly |
Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):
| Stage | LR class | MCA mean | MCA prop. zeros |
|---|---|---|---|
| Sporozoite | not expressed | N/A | N/A |
| Ring | not expressed | 0.01 | 0.99 |
| Trophozoite | possibly expressed | 0.10 | 0.94 |
| Schizont | not expressed | 0.04 | 0.98 |
| Gametocyte | not expressed | 0.17 | 0.90 |
More info:
Old (Pf3D7v3) Gene ID: PF3D7_1435800
Resistome Missense Mutations: None
Resistome Compounds with Missense Mutations: None
Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)
Zhang Phenotype: Non - Mutable in CDS
MIS: 0.505 | MFS: -2.547 | #Insertions: 0
PlasmoGEM Phenotype: N/A
RMgmDB ABS Phenotype: N/A
More info: PhenoPlasm Link
AlphaFill Uniprot ID: Q8ILA6
"Best" AlphaFill ligand hit: GNP (phosphoaminophosphonic acid-guanylate ester, Local RMSD=0.11) with 3A1U (Global RMSD=5.90)
BRENDA EC Inhibitors:
| EC # | Name | EC Inhibitors |
|---|---|---|
| 3.6.5.2 | small monomeric GTPase | GTPGDPEDTAPlexAstatinCCG-977Rap1GapNSC23766CCG-1423Rap1GAP1GTPgammaSCalmodulin... |
No evidence of orthology to BindingDB entries
Ortholog Group (OrthoMCL): OG6_101468
Most Similar Human Ortholog: K7EQJ8
TM-align score: 0.75 | RMSD: 2.57
Seq Identity: 0.15 | Length: 78 / 504
All Human Orthologs (OrthoMCL):
| Gene ID | Description |
|---|---|
| ENSG00000132591 | Era like 12S mitochondrial rRNA chaperone 1 |
MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:
Homozygous genotype calls only
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 3 | 34 | 18 | 21 |
| disruptive | 9 | 36 | 26 | 76 |
| missense | 9 | 34 | 25 | 71 |
Any inclusion in genotype call
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 8 | 58 | 18 | 42 |
| disruptive | 17 | 97 | 54 | 122 |
| missense | 13 | 85 | 51 | 90 |
PlasmoDB Total SNPs: 93
Non-coding: 72 | Synonymous: 13 | Nonsynonymous: 8 | Stop Codon: 0
Protein Length: 504 | Molecular Weight (kDa): 59.258
UniProt ID(s): Q8ILA6
PDB ID(s): None
Isoelectric Point: 9.53
Protein Domain Annotations:
| Source | Family ID | Description |
|---|---|---|
| InterPro | IPR006073 | GTP binding domain |
| InterPro | IPR009019 | K homology domain superfamily, prokaryotic type |
| InterPro | IPR027417 | P-loop containing nucleoside triphosphate hydrolase |
| InterPro | IPR030388 | Era-type guanine nucleotide-binding (G) domain |
| PFam | PF01926 | GTP binding domain |
| Superfamily | SSF52540 | P-loop containing nucleoside triphosphate hydrolase |
| Superfamily | SSF54814 | K homology domain superfamily, prokaryotic type |
| PMID | Title | Authors | DOI/Link |
|---|---|---|---|
| 12368864 | Genome sequence of the human malaria parasite Plasmodium falciparum. | Gardner MJ, Hall N, ..., Barrell B | 10.1038/nature01097 |
| 24850912 | Reduced ribosomes of the apicoplast and mitochondrion of Plasmodium spp. andpredicted interactions with antibiotics. | Gupta A, Shah P, ..., Habib S | 10.1098/rsob.140045 |
| 34494883 | A Prioritized and Validated Resource of Mitochondrial Proteins in PlasmodiumIdentifies Unique Biology. | van Esveld SL, Meerstein-Kessel L, ..., Huynen MA | 10.1128/mSphere.00614-21 |