Genome location: Pf3D7_14_v3:1,456,374..1,460,503(+)
Genome classification: Core
Product Description: glucose-6-phosphate isomerase
SignalP Peptide: N/A
# Transmembrane Domains: 0
EC Numbers: 5.3.1.9 (Glucose-6-phosphate isomerase)
Curated GO (PlasmoDB):
| Type | GO Term | Name |
|---|---|---|
| Component | GO:0020036 | Maurer's cleft |
| Component | GO:0005829 | cytosol |
| Function | GO:0004347 | glucose-6-phosphate isomerase activity |
| Function | GO:0048029 | monosaccharide binding |
| Process | GO:0006094 | gluconeogenesis |
| Process | GO:0051156 | glucose 6-phosphate metabolic process |
| Process | GO:0006096 | glycolytic process |
| Process | GO:0006098 | pentose-phosphate shunt |
Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):
| Stage | LR class | MCA mean | MCA prop. zeros |
|---|---|---|---|
| Sporozoite | not expressed | N/A | N/A |
| Ring | expressed | 0.40 | 0.81 |
| Trophozoite | expressed | 0.74 | 0.61 |
| Schizont | expressed | 0.07 | 0.96 |
| Gametocyte | expressed | 0.28 | 0.84 |
More info:
Old (Pf3D7v3) Gene ID: PF3D7_1436000
Resistome Missense Mutations: None
Resistome Compounds with Missense Mutations: None
Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)
Zhang Phenotype: Non - Mutable in CDS
MIS: 0.177 | MFS: -3.115 | #Insertions: 0
PlasmoGEM Phenotype: Essential (Pb ortholog: PBANKA_1008800)
RMgmDB ABS Phenotype: N/A
More info: PhenoPlasm Link
AlphaFill Uniprot ID: Q8ILA4
"Best" AlphaFill ligand hit: G6Q (glucose-6-phosphate, Local RMSD=0.12) with 3UJH (Global RMSD=2.90)
BRENDA EC Inhibitors:
| EC # | Name | EC Inhibitors |
|---|---|---|
| 5.3.1.9 | glucose-6-phosphate isomerase | GTPEDTAMaleatesuraminmalonatephosphateoxaloacetateoxoglutarateAgaricic acidMaleic anhydride3-phosphoglycerate6-phosphogluconate... |
Orthology to BindingDB Entries:
| UniProt | Protein Name | Species | Homology Source | BindingDB Ligands |
|---|---|---|---|---|
| Q9N1E2 | Glucose-6-phosphate isomerase | Oryctolagus cuniculus | OrthoDB | CHEMBL1235136 |
Ortholog Group (OrthoMCL): OG6_100716
Most Similar Human Ortholog: P06744
TM-align score: 0.95 | RMSD: 1.38
Seq Identity: 0.38 | Length: 541 / 579
All Human Orthologs (OrthoMCL):
| Gene ID | Description |
|---|---|
| ENSG00000105220 | glucose-6-phosphate isomerase |
| ENSG00000282019 | glucose-6-phosphate isomerase |
Protein Length: 579 | Molecular Weight (kDa): 67.368
Isoelectric Point: 7.21
Protein Domain Annotations:
| Source | Family ID | Description |
|---|---|---|
| InterPro | IPR001672 | Phosphoglucose isomerase (PGI) |
| InterPro | IPR018189 | Phosphoglucose isomerase, conserved site |
| InterPro | IPR035476 | Phosphoglucose isomerase, SIS domain 1 |
| InterPro | IPR035482 | Phosphoglucose isomerase, SIS domain 2 |
| PFam | PF00342 | Phosphoglucose isomerase (PGI) |
| Superfamily | SSF53697 | N/A |
MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:
Homozygous genotype calls only
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 4 | 25 | 12 | 54 |
| disruptive | 4 | 37 | 28 | 71 |
| missense | 4 | 36 | 28 | 62 |
Any inclusion in genotype call
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 8 | 60 | 24 | 37 |
| disruptive | 12 | 90 | 61 | 131 |
| missense | 8 | 76 | 54 | 94 |
PlasmoDB Total SNPs: 135
Non-coding: 108 | Synonymous: 18 | Nonsynonymous: 8 | Stop Codon: 1
| PMID | Title | Authors | DOI/Link |
|---|---|---|---|
| 2197273 | Cloning metabolic pathway genes by complementation in Escherichia coli. Isolationand expression of Plasmodium falciparum glucose phosphate isomerase. | Kaslow DC, Hill S | https://pubmed.ncbi.nlm.nih.gov/2197273/ |
| 12368864 | Genome sequence of the human malaria parasite Plasmodium falciparum. | Gardner MJ, Hall N, ..., Barrell B | 10.1038/nature01097 |
| 16267556 | A protein interaction network of the malaria parasite Plasmodium falciparum. | LaCount DJ, Vignali M, ..., Hughes RE | 10.1038/nature04104 |
| 31631203 | A network-based approach reveals novel invasion and Maurer's clefts-relatedproteins in Plasmodium falciparum. | Das D, Krishnan SR, Roy A, Bulusu G | 10.1039/c9mo00124g |
| 38396332 | Overcoming the egress block of Plasmodium sporozoites expressing fluorescentlytagged circumsporozoite protein | Thieleke-Matos C, Walz K, Frischknecht F, Singer M | 10.1111/mmi.15230 |
| 38395606 | Protein sorting upon exit from the endoplasmic reticulum dominates Golgibiogenesis in budding yeast | Sasaki S, Schlarmann P, ..., Funato K | 10.1002/1873-3468.14830 |
| 38360646 | Stable expression of mucin glycoproteins GP40 and GP15 of Cryptosporidium parvumin Toxoplasma gondii | Li M, Sun X, ..., Guo Y | 10.1186/s13071-024-06159-y |