Genome location: Pf3D7_14_v3:1,476,538..1,483,887(+)
Genome classification: Core
Product Description: translocon component PTEX150
SignalP Peptide: MRIIILALLIVCTIINYYCA
# Transmembrane Domains: 0
EC Numbers: None
Curated GO (PlasmoDB):
| Type | GO Term | Name |
|---|---|---|
| Component | GO:0097619 | PTEX complex |
| Component | GO:0020007 | apical complex |
| Component | GO:1903561 | extracellular vesicle |
| Component | GO:0020026 | merozoite dense granule |
| Component | GO:0005634 | nucleus |
| Component | GO:0020005 | symbiont-containing vacuole membrane |
| Function | GO:0005515 | protein binding |
| Process | GO:0044053 | translocation of peptides or proteins into host cell cytoplasm |
Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):
| Stage | LR class | MCA mean | MCA prop. zeros |
|---|---|---|---|
| Sporozoite | expressed | N/A | N/A |
| Ring | expressed | 3.06 | 0.07 |
| Trophozoite | expressed | 0.99 | 0.53 |
| Schizont | expressed | 0.76 | 0.60 |
| Gametocyte | expressed | 1.02 | 0.49 |
More info:
Old (Pf3D7v3) Gene ID: PF3D7_1436300
Resistome Missense Mutations: None
Resistome Compounds with Missense Mutations: None
Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)
Zhang Phenotype: Mutable in CDS
MIS: 1 | MFS: -2.182 | #Insertions: 2
PlasmoGEM Phenotype: N/A
RMgmDB ABS Phenotype: Different from wild type (Pb ortholog: PBANKA_1008500)
Modification: Tagged | RMgm-5276
More info: PhenoPlasm Link
AlphaFill Uniprot ID: Q8ILA1
"Best" AlphaFill ligand hit: No AlphaFill hits
No associated EC numbersNo evidence of orthology to BindingDB entries
MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:
Homozygous genotype calls only
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 6 | 37 | 17 | 58 |
| disruptive | 47 | 113 | 66 | 157 |
| missense | 41 | 95 | 61 | 118 |
Any inclusion in genotype call
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 10 | 76 | 36 | 57 |
| disruptive | 67 | 260 | 124 | 312 |
| missense | 54 | 211 | 96 | 162 |
PlasmoDB Total SNPs: 273
Non-coding: 211 | Synonymous: 26 | Nonsynonymous: 36 | Stop Codon: 0
| PMID | Title | Authors | DOI/Link |
|---|---|---|---|
| 12368864 | Genome sequence of the human malaria parasite Plasmodium falciparum. | Gardner MJ, Hall N, ..., Barrell B | 10.1038/nature01097 |
| 16267556 | A protein interaction network of the malaria parasite Plasmodium falciparum. | LaCount DJ, Vignali M, ..., Hughes RE | 10.1038/nature04104 |
| 19536257 | A newly discovered protein export machine in malaria parasites. | de Koning-Ward TF, Gilson PR, ..., Crabb BS | 10.1038/nature08104 |
| 22253438 | Biosynthesis, localization, and macromolecular arrangement of the Plasmodiumfalciparum translocon of exported proteins (PTEX). | Bullen HE, Charnaud SC, ..., Gilson PR | 10.1074/jbc.M111.328591 |
| 25043043 | PTEX is an essential nexus for protein export in malaria parasites. | Elsworth B, Matthews K, ..., de Koning-Ward TF | 10.1038/nature13555 |
| 28944300 | Proteomic analysis of extracellular vesicles from a Plasmodium falciparum Kenyanclinical isolate defines a core parasite secretome. | Abdi A, Yu L, ..., Rayner J | 10.12688/wellcomeopenres.11910.2 |
| 30150771 | Malaria parasite translocon structure and mechanism of effector export. | Ho CM, Beck JR, ..., Zhou ZH | 10.1038/s41586-018-0469-4 |
| 30379851 | Identification of Plasmodium falciparum nuclear proteins by mass spectrometry andproposed protein annotation. | Briquet S, Ourimi A, ..., Vaquero C | 10.1371/journal.pone.0205596 |
| 35192672 | A revised mechanism for how Plasmodium falciparum recruits and exports proteinsinto its erythrocytic host cell. | Gabriela M, Matthews KM, ..., Gilson PR | 10.1371/journal.ppat.1009977 |
| 24076174 | Two putative protein export regulators promote Plasmodium blood stage developmentin vivo | Matz JM, Matuschewski K, Kooij TW | 10.1016/j.molbiopara.2013.09.003 |
| 27865834 | Expression and characterization of the Plasmodium translocon of the exportedproteins component EXP2 | Hakamada K, Watanabe H, ..., Yohda M | 10.1016/j.bbrc.2016.11.097 |
| 22970262 | An exported heat shock protein 40 associates with pathogenesis-related knobs inPlasmodium falciparum infected erythrocytes | Acharya P, Chaubey S, Grover M, Tatu U | 10.1371/journal.pone.0044605 |
| 37523385 | PTEX helps efficiently traffic haemoglobinases to the food vacuole in Plasmodiumfalciparum | Jonsdottir TK, Elsworth B, ..., Gilson PR | 10.1371/journal.ppat.1011006 |
| 27019089 | Proteomic analysis reveals novel proteins associated with the Plasmodium proteinexporter PTEX and a loss of complex stability upon truncation of the core PTEXcomponent, PTEX150 | Elsworth B, Sanders PR, ..., Gilson PR | 10.1111/cmi.12596 |
| 37552754 | Knock-sideways by inducible ER retrieval enables a unique approach for studyingPlasmodium-secreted proteins | Fierro MA, Hussain T, Campin LJ, Beck JR | 10.1073/pnas.2308676120 |
| 33901679 | Plasmodium falciparum SURFIN(4.1) forms an intermediate complex with PTEXcomponents and Pf113 during export to the red blood cell | Miyazaki S, Chitama BA, ..., Kaneko O | 10.1016/j.parint.2021.102358 |
| 30476118 | The N-terminus of EXP2 forms the membrane-associated pore of the proteinexporting translocon PTEX in Plasmodium falciparum | Sanders PR, Dickerman BK, ..., Gilson PR | 10.1093/jb/mvy099 |
| 30150733 | EXP2 is a nutrient-permeable channel in the vacuolar membrane of Plasmodium andis essential for protein export via PTEX | Garten M, Nasamu AS, ..., Beck JR | 10.1038/s41564-018-0222-7 |
| 29696751 | Spatial organization of protein export in malaria parasite blood stages | Charnaud SC, Jonsdottir TK, ..., Gilson PR | 10.1111/tra.12577 |
| 22996664 | Complete Plasmodium falciparum liver-stage development in liver-chimeric mice | Vaughan AM, Mikolajczak SA, ..., Kappe SH | 10.1172/JCI62684 |
| 32521667 | Crossing the Vacuolar Rubicon: Structural Insights into Effector ProteinTrafficking in Apicomplexan Parasites | Egea PF | 10.3390/microorganisms8060865 |
| 23869529 | The Plasmodium translocon of exported proteins (PTEX) component thioredoxin-2 isimportant for maintaining normal blood-stage growth | Matthews K, Kalanon M, ..., de Koning-Ward TF | 10.1111/mmi.12334 |
| 34956312 | Absence of PEXEL-Dependent Protein Export in Plasmodium Liver Stages Cannot BeRestored by Gain of the HSP101 Protein Translocon ATPase | Kreutzfeld O, Grutzke J, ..., Matuschewski K | 10.3389/fgene.2021.742153 |
| 31289187 | Deletion of Plasmodium falciparum Protein RON3 Affects the FunctionalTranslocation of Exported Proteins and Glucose Uptake | Low LM, Azasi Y, ..., Miller LH | 10.1128/mBio.01460-19 |
| 29637707 | The malaria PTEX component PTEX88 interacts most closely with HSP101 at thehost-parasite interface | Chisholm SA, Kalanon M, ..., de Koning-Ward TF | 10.1111/febs.14463 |