PF3D7_1436500

Genome location: Pf3D7_14_v3:1,488,559..1,490,278(+)

Genome classification: Core

Function and Localization

Product Description: GTP-binding protein, putative

SignalP Peptide: N/A

# Transmembrane Domains: 0

EC Numbers: None

Curated GO (PlasmoDB):

Type	GO Term	Name
Component	GO:0005739	mitochondrion
Function	GO:0003924	GTPase activity

Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):

Stage	LR class	MCA mean	MCA prop. zeros
Sporozoite	possibly expressed	N/A	N/A
Ring	expressed	0.02	0.99
Trophozoite	expressed	0.20	0.87
Schizont	expressed	0.06	0.95
Gametocyte	expressed	0.16	0.90

More info:

Resistome Mutations

Old (Pf3D7v3) Gene ID: PF3D7_1436500

Resistome Missense Mutations: None

Resistome Compounds with Missense Mutations: None

Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)

Essentiality (ABS)

Zhang Phenotype: Non - Mutable in CDS

MIS: 0.143 | MFS: -3.217 | #Insertions: 0

PlasmoGEM Phenotype: Essential (Pb ortholog: PBANKA_1235500)

Relative Growth Rate: 0.06 ± 0.07
Confidence: 6.78

RMgmDB ABS Phenotype: N/A

More info: PhenoPlasm Link

Binding Evidence

AlphaFill Uniprot ID: Q8ILA0

"Best" AlphaFill ligand hit: GDP (guanosine-5'-diphosphate, Local RMSD=0.63) with 6G0Z (Global RMSD=9.23)

No associated EC numbers

No evidence of orthology to BindingDB entries

Orthology Information

Ortholog Group (OrthoMCL): OG6_101533

Most Similar Human Ortholog: Q9BT17

TM-align score: 0.83 | RMSD: 2.57

Seq Identity: 0.29 | Length: 305 / 431

All Human Orthologs (OrthoMCL):

Gene ID	Description
ENSG00000148824	mitochondrial ribosome associated GTPase 1

Genetic Variation

MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:

Homozygous genotype calls only

variant type	common	rare	doubleton	singleton
synonymous	4	18	14	24
disruptive	11	49	24	72
missense	4	42	18	59

Any inclusion in genotype call

variant type	common	rare	doubleton	singleton
synonymous	7	39	20	27
disruptive	21	89	48	103
missense	9	72	38	74

PlasmoDB Total SNPs: 77

Non-coding: 57 | Synonymous: 14 | Nonsynonymous: 6 | Stop Codon: 0

Protein Information

Protein Length: 431 | Molecular Weight (kDa): 49.914

UniProt ID(s): Q8ILA0

PDB ID(s): None

Isoelectric Point: 9.95

Protein Domain Annotations:

Source	Family ID	Description
InterPro	IPR006073	GTP binding domain
InterPro	IPR027417	P-loop containing nucleoside triphosphate hydrolase
PFam	PF01926	GTP binding domain
Superfamily	SSF52540	P-loop containing nucleoside triphosphate hydrolase

PMID	Title	Authors	DOI/Link
12368864	Genome sequence of the human malaria parasite Plasmodium falciparum.	Gardner MJ, Hall N, ..., Barrell B	10.1038/nature01097
34494883	A Prioritized and Validated Resource of Mitochondrial Proteins in PlasmodiumIdentifies Unique Biology.	van Esveld SL, Meerstein-Kessel L, ..., Huynen MA	10.1128/mSphere.00614-21