About Gene List

PF3D7_1437200

Genome location: Pf3D7_14_v3:1,506,995..1,511,703(-)

Genome classification: Core

Function and Localization

Product Description: ribonucleoside-diphosphate reductase large subunit, putative

SignalP Peptide: N/A

# Transmembrane Domains: 0

EC Numbers: 1.17.4.1 (Ribonucleoside-diphosphate reductase)

Curated GO (PlasmoDB):

Type GO Term Name
Component GO:0020011 apicoplast
Component GO:0005634 nucleus
Component GO:0005971 ribonucleoside-diphosphate reductase complex
Function GO:0005524 ATP binding
Function GO:0004748 ribonucleoside-diphosphate reductase activity, thioredoxin disulfide as acceptor
Process GO:0006260 DNA replication
Process GO:0009263 deoxyribonucleotide biosynthetic process

Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):

Stage LR class MCA mean MCA prop. zeros
Sporozoite expressed N/A N/A
Ring expressed 0.11 0.95
Trophozoite expressed 0.66 0.64
Schizont expressed 0.81 0.55
Gametocyte expressed 0.58 0.72

More info:

Resistome Mutations

Old (Pf3D7v3) Gene ID: PF3D7_1437200

Resistome Missense Mutations: None

Resistome Compounds with Missense Mutations: None

Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)

Essentiality (ABS)

Zhang Phenotype: Non - Mutable in CDS

MIS: 0.14 | MFS: -2.765 | #Insertions: 0

PlasmoGEM Phenotype: Essential (Pb ortholog: PBANKA_0611600)

  • Relative Growth Rate: 0.10 ± nan
  • Confidence: 1.00

RMgmDB ABS Phenotype: N/A

More info: PhenoPlasm Link

Binding Evidence

AlphaFill Uniprot ID: Q8IL94

"Best" AlphaFill ligand hit: DTP (, Local RMSD=0.21) with 5CNT (Global RMSD=3.62)

BRENDA EC Inhibitors:

EC # Name EC Inhibitors
1.17.4.1 ribonucleoside-diphosphate reductase BRENDA SOAP query unsuccessful

Orthology to BindingDB Entries:

UniProt Protein Name Species Homology Source BindingDB Ligands
P07742 Ribonucleoside-diphosphate reductase large subunit Mus musculus HOGENOM, OMA, OrthoDB, OrthoMCL BLAST CHEMBL1790913CHEMBL1790907CHEMBL1790908
P23921 Ribonucleoside-diphosphate reductase large subunit Homo sapiens HOGENOM, OMA, OrthoDB, OrthoMCL BLAST TriapineUS10155732, Compound COH20US11634395, Example 1US11634395, Example 3...

Orthology Information

Ortholog Group (OrthoMCL): OG6_100448

Most Similar Human Ortholog: P23921

TM-align score: 0.97 | RMSD: 1.73

Seq Identity: 0.65 | Length: 784 / 847

All Human Orthologs (OrthoMCL):

Gene ID Description
ENSG00000167325 ribonucleotide reductase catalytic subunit M1

Protein Information

Protein Length: 847 | Molecular Weight (kDa): 96.904

UniProt ID(s): Q8IL94

PDB ID(s): None

Isoelectric Point: 7.17

Protein Domain Annotations:

Source Family ID Description
InterPro IPR000788 Ribonucleotide reductase large subunit, C-terminal
InterPro IPR005144 ATP-cone domain
InterPro IPR008926 Ribonucleotide reductase R1 subunit, N-terminal
InterPro IPR013346 Ribonucleotide reductase, class I , alpha subunit
InterPro IPR013509 Ribonucleotide reductase large subunit, N-terminal
InterPro IPR039718 Ribonucleoside-diphosphate reductase large subunit
PFam PF00317 Ribonucleotide reductase large subunit, N-terminal
PFam PF02867 Ribonucleotide reductase large subunit, C-terminal
PFam PF03477 ATP-cone domain
Superfamily SSF48168 Ribonucleotide reductase R1 subunit, N-terminal
Superfamily SSF51998

Genetic Variation

MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:

Homozygous genotype calls only

variant type common rare doubleton singleton
synonymous 4 66 37 64
disruptive 3 10 12 56
missense 3 10 11 44

Any inclusion in genotype call

variant type common rare doubleton singleton
synonymous 14 151 45 49
disruptive 7 52 44 143
missense 5 40 35 89

PlasmoDB Total SNPs: 218

Non-coding: 201 | Synonymous: 16 | Nonsynonymous: 1 | Stop Codon: 0

Associated Publications

PMID Title Authors DOI/Link
12368864 Genome sequence of the human malaria parasite Plasmodium falciparum. Gardner MJ, Hall N, ..., Barrell B 10.1038/nature01097
16267556 A protein interaction network of the malaria parasite Plasmodium falciparum. LaCount DJ, Vignali M, ..., Hughes RE 10.1038/nature04104
23181666 Organellar proteomics reveals hundreds of novel nuclear proteins in the malariaparasite Plasmodium falciparum. Oehring SC, Woodcroft BJ, ..., Voss TS 10.1186/gb-2012-13-11-r108
30212465 Integrative proteomics and bioinformatic prediction enable a high-confidenceapicoplast proteome in malaria parasites. Boucher MJ, Ghosh S, ..., Yeh E 10.1371/journal.pbio.2005895