Product Description: ribonucleoside-diphosphate reductase large subunit, putative
SignalP Peptide: N/A
# Transmembrane Domains: 0
EC Numbers: 1.17.4.1 (Ribonucleoside-diphosphate reductase)
Curated GO (PlasmoDB):
| Type | GO Term | Name |
|---|---|---|
| Component | GO:0020011 | apicoplast |
| Component | GO:0005634 | nucleus |
| Component | GO:0005971 | ribonucleoside-diphosphate reductase complex |
| Function | GO:0005524 | ATP binding |
| Function | GO:0004748 | ribonucleoside-diphosphate reductase activity, thioredoxin disulfide as acceptor |
| Process | GO:0006260 | DNA replication |
| Process | GO:0009263 | deoxyribonucleotide biosynthetic process |
Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):
| Stage | LR class | MCA mean | MCA prop. zeros |
|---|---|---|---|
| Sporozoite | expressed | N/A | N/A |
| Ring | expressed | 0.11 | 0.95 |
| Trophozoite | expressed | 0.66 | 0.64 |
| Schizont | expressed | 0.81 | 0.55 |
| Gametocyte | expressed | 0.58 | 0.72 |
More info:
Old (Pf3D7v3) Gene ID: PF3D7_1437200
Resistome Missense Mutations: None
Resistome Compounds with Missense Mutations: None
Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)
Zhang Phenotype: Non - Mutable in CDS
MIS: 0.14 | MFS: -2.765 | #Insertions: 0
PlasmoGEM Phenotype: Essential (Pb ortholog: PBANKA_0611600)
RMgmDB ABS Phenotype: N/A
More info: PhenoPlasm Link
AlphaFill Uniprot ID: Q8IL94
"Best" AlphaFill ligand hit: DTP (, Local RMSD=0.21) with 5CNT (Global RMSD=3.62)
BRENDA EC Inhibitors:
| EC # | Name | EC Inhibitors |
|---|---|---|
| 1.17.4.1 | ribonucleoside-diphosphate reductase | BRENDA SOAP query unsuccessful |
Orthology to BindingDB Entries:
| UniProt | Protein Name | Species | Homology Source | BindingDB Ligands |
|---|---|---|---|---|
| P07742 | Ribonucleoside-diphosphate reductase large subunit | Mus musculus | HOGENOM, OMA, OrthoDB, OrthoMCL BLAST | CHEMBL1790913CHEMBL1790907CHEMBL1790908 |
| P23921 | Ribonucleoside-diphosphate reductase large subunit | Homo sapiens | HOGENOM, OMA, OrthoDB, OrthoMCL BLAST | TriapineUS10155732, Compound COH20US11634395, Example 1US11634395, Example 3... |
Ortholog Group (OrthoMCL): OG6_100448
Most Similar Human Ortholog: P23921
TM-align score: 0.97 | RMSD: 1.73
Seq Identity: 0.65 | Length: 784 / 847
All Human Orthologs (OrthoMCL):
| Gene ID | Description |
|---|---|
| ENSG00000167325 | ribonucleotide reductase catalytic subunit M1 |
Protein Length: 847 | Molecular Weight (kDa): 96.904
UniProt ID(s): Q8IL94
PDB ID(s): None
Isoelectric Point: 7.17
Protein Domain Annotations:
| Source | Family ID | Description |
|---|---|---|
| InterPro | IPR000788 | Ribonucleotide reductase large subunit, C-terminal |
| InterPro | IPR005144 | ATP-cone domain |
| InterPro | IPR008926 | Ribonucleotide reductase R1 subunit, N-terminal |
| InterPro | IPR013346 | Ribonucleotide reductase, class I , alpha subunit |
| InterPro | IPR013509 | Ribonucleotide reductase large subunit, N-terminal |
| InterPro | IPR039718 | Ribonucleoside-diphosphate reductase large subunit |
| PFam | PF00317 | Ribonucleotide reductase large subunit, N-terminal |
| PFam | PF02867 | Ribonucleotide reductase large subunit, C-terminal |
| PFam | PF03477 | ATP-cone domain |
| Superfamily | SSF48168 | Ribonucleotide reductase R1 subunit, N-terminal |
| Superfamily | SSF51998 |
MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:
Homozygous genotype calls only
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 4 | 66 | 37 | 64 |
| disruptive | 3 | 10 | 12 | 56 |
| missense | 3 | 10 | 11 | 44 |
Any inclusion in genotype call
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 14 | 151 | 45 | 49 |
| disruptive | 7 | 52 | 44 | 143 |
| missense | 5 | 40 | 35 | 89 |
PlasmoDB Total SNPs: 218
Non-coding: 201 | Synonymous: 16 | Nonsynonymous: 1 | Stop Codon: 0
| PMID | Title | Authors | DOI/Link |
|---|---|---|---|
| 12368864 | Genome sequence of the human malaria parasite Plasmodium falciparum. | Gardner MJ, Hall N, ..., Barrell B | 10.1038/nature01097 |
| 16267556 | A protein interaction network of the malaria parasite Plasmodium falciparum. | LaCount DJ, Vignali M, ..., Hughes RE | 10.1038/nature04104 |
| 23181666 | Organellar proteomics reveals hundreds of novel nuclear proteins in the malariaparasite Plasmodium falciparum. | Oehring SC, Woodcroft BJ, ..., Voss TS | 10.1186/gb-2012-13-11-r108 |
| 30212465 | Integrative proteomics and bioinformatic prediction enable a high-confidenceapicoplast proteome in malaria parasites. | Boucher MJ, Ghosh S, ..., Yeh E | 10.1371/journal.pbio.2005895 |