PF3D7_1437400 (PANK2)

Genome location: Pf3D7_14_v3:1,517,047..1,519,845(+)

Genome classification: Core

Function and Localization

Product Description: pantothenate kinase 2

SignalP Peptide: N/A

# Transmembrane Domains: 0

EC Numbers: 2.7.1.33 (Pantothenate kinase)

Curated GO (PlasmoDB):

Type	GO Term	Name
Component	GO:0005829	cytosol
Component	GO:0005634	nucleus
Function	GO:0071889	14-3-3 protein binding
Function	GO:0004594	pantothenate kinase activity
Function	GO:0005515	protein binding
Process	GO:0015937	coenzyme A biosynthetic process

Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):

Stage	LR class	MCA mean	MCA prop. zeros
Sporozoite	not expressed	N/A	N/A
Ring	not expressed	0.12	0.94
Trophozoite	possibly expressed	0.72	0.61
Schizont	possibly expressed	0.23	0.85
Gametocyte	not expressed	0.19	0.89

More info:

Resistome Mutations

Old (Pf3D7v3) Gene ID: PF3D7_1437400

Resistome Missense Mutations: None

Resistome Compounds with Missense Mutations: None

Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)

Essentiality (ABS)

Zhang Phenotype: Mutable in CDS

MIS: 0.92 | MFS: -2.929 | #Insertions: 1

PlasmoGEM Phenotype: Dispensable (Pb ortholog: PBANKA_0611400)

Relative Growth Rate: 0.91 ± 0.18
Confidence: 4.84

RMgmDB ABS Phenotype: Not different from wild type (Pb ortholog: PBANKA_0611400)

Modification: Disrupted | RMgm-4085

More info: PhenoPlasm Link

Binding Evidence

AlphaFill Uniprot ID: Q8IL92

"Best" AlphaFill ligand hit: ACO (acetyl coenzyme *a, Local RMSD=0.18) with 3MK6 (Global RMSD=2.26)

BRENDA EC Inhibitors:

EC #	Name	EC Inhibitors
2.7.1.33	pantothenate kinase	ATPADPCoAAMPEDTANH4+HgCl2AlCl3GW5074citrateMCC-555neomycin...

No evidence of orthology to BindingDB entries

Orthology Information

Ortholog Group (OrthoMCL): OG6_532760

No human ortholog(s)

Genetic Variation

MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:

Homozygous genotype calls only

variant type	common	rare	doubleton	singleton
synonymous	7	29	16	58
disruptive	14	116	73	166
missense	13	111	73	154

Any inclusion in genotype call

variant type	common	rare	doubleton	singleton
synonymous	11	70	39	55
disruptive	31	256	128	229
missense	25	236	110	175

PlasmoDB Total SNPs: 72

Non-coding: 21 | Synonymous: 22 | Nonsynonymous: 28 | Stop Codon: 1

Protein Information

Protein Length: 766 | Molecular Weight (kDa): 91.042

UniProt ID(s): Q8IL92

PDB ID(s): None

Isoelectric Point: 8.42

Protein Domain Annotations:

Source	Family ID	Description
InterPro	IPR004567	Type II pantothenate kinase
InterPro	IPR043129	ATPase, nucleotide binding domain
PFam	PF03630	Type II pantothenate kinase
Superfamily	SSF53067	ATPase, nucleotide binding domain

PMID	Title	Authors	DOI/Link
12368864	Genome sequence of the human malaria parasite Plasmodium falciparum.	Gardner MJ, Hall N, ..., Barrell B	10.1038/nature01097
34324601	A novel heteromeric pantothenate kinase complex in apicomplexan parasites.	Tjhin ET, Howieson VM, ..., Saliba KJ	10.1371/journal.ppat.1009797