Product Description: succinyl-CoA ligase, putative
SignalP Peptide: N/A
# Transmembrane Domains: 0
EC Numbers: 6.2.1.5 (Succinate--CoA ligase (ADP-forming))
Curated GO (PlasmoDB):
| Type | GO Term | Name |
|---|---|---|
| Component | GO:0005739 | mitochondrion |
| Component | GO:0005634 | nucleus |
| Function | GO:0003987 | acetate-CoA ligase activity |
| Process | GO:0006096 | glycolytic process |
| Process | GO:0006099 | tricarboxylic acid cycle |
Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):
| Stage | LR class | MCA mean | MCA prop. zeros |
|---|---|---|---|
| Sporozoite | not expressed | N/A | N/A |
| Ring | not expressed | 0.05 | 0.98 |
| Trophozoite | not expressed | 0.14 | 0.91 |
| Schizont | not expressed | 0.13 | 0.91 |
| Gametocyte | not expressed | 0.33 | 0.80 |
More info:
Old (Pf3D7v3) Gene ID: PF3D7_1437700
Resistome Missense Mutations: None
Resistome Compounds with Missense Mutations: None
Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)
Zhang Phenotype: Mutable in CDS
MIS: 1 | MFS: -2.269 | #Insertions: 5
PlasmoGEM Phenotype: Dispensable (Pb ortholog: PBANKA_0611100)
RMgmDB ABS Phenotype: Different from wild type (Pb ortholog: PBANKA_0611100)
Modification: Disrupted | RMgm-2100
More info: PhenoPlasm Link
AlphaFill Uniprot ID: Q8IL89
"Best" AlphaFill ligand hit: COA (coenzyme a, Local RMSD=0.07) with 4XYM (Global RMSD=2.21)
BRENDA EC Inhibitors:
| EC # | Name | EC Inhibitors |
|---|---|---|
| 6.2.1.5 | succinate-CoA ligase (ADP-forming) | BRENDA SOAP query unsuccessful |
No evidence of orthology to BindingDB entries
MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:
Homozygous genotype calls only
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 5 | 62 | 32 | 60 |
| disruptive | 18 | 101 | 53 | 118 |
| missense | 18 | 100 | 52 | 103 |
Any inclusion in genotype call
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 19 | 104 | 36 | 78 |
| disruptive | 40 | 169 | 77 | 181 |
| missense | 34 | 159 | 68 | 142 |
PlasmoDB Total SNPs: 103
Non-coding: 45 | Synonymous: 34 | Nonsynonymous: 24 | Stop Codon: 0
Protein Length: 973 | Molecular Weight (kDa): 111.279
UniProt ID(s): Q8IL89
PDB ID(s): None
Isoelectric Point: 8.09
Protein Domain Annotations:
| Source | Family ID | Description |
|---|---|---|
| InterPro | IPR003781 | CoA-binding |
| InterPro | IPR016102 | Succinyl-CoA synthetase-like |
| InterPro | IPR032875 | Succinyl-CoA synthetase-like, flavodoxin domain |
| InterPro | IPR036291 | NAD(P)-binding domain superfamily |
| InterPro | IPR043938 | Ligase-CoA domain |
| PFam | PF13380 | CoA-binding |
| PFam | PF13549 | Succinyl-CoA synthetase-like, flavodoxin domain |
| PFam | PF13607 | Ligase-CoA domain |
| PFam | PF19045 | |
| Superfamily | SSF51735 | NAD(P)-binding domain superfamily |
| Superfamily | SSF52210 | Succinyl-CoA synthetase-like |
| Superfamily | SSF56059 |
| PMID | Title | Authors | DOI/Link |
|---|---|---|---|
| 12368864 | Genome sequence of the human malaria parasite Plasmodium falciparum. | Gardner MJ, Hall N, ..., Barrell B | 10.1038/nature01097 |
| 23181666 | Organellar proteomics reveals hundreds of novel nuclear proteins in the malariaparasite Plasmodium falciparum. | Oehring SC, Woodcroft BJ, ..., Voss TS | 10.1186/gb-2012-13-11-r108 |
| 34494883 | A Prioritized and Validated Resource of Mitochondrial Proteins in PlasmodiumIdentifies Unique Biology. | van Esveld SL, Meerstein-Kessel L, ..., Huynen MA | 10.1128/mSphere.00614-21 |
| 35984838 | Mitochondrially targeted proximity biotinylation and proteomic analysis inPlasmodium falciparum. | Lamb IM, Rios KT, ..., Vaidya AB | 10.1371/journal.pone.0273357 |