Genome location: Pf3D7_14_v3:1,531,955..1,536,263(+)
Genome classification: Core
Product Description: HSP40, subfamily A
SignalP Peptide: N/A
# Transmembrane Domains: 0
EC Numbers: None
Curated GO (PlasmoDB):
| Type | GO Term | Name |
|---|---|---|
| Component | GO:0005829 | cytosol |
| Component | GO:0020002 | host cell plasma membrane |
| Component | GO:0016020 | membrane |
| Component | GO:0005634 | nucleus |
| Function | GO:0016887 | ATP hydrolysis activity |
| Function | GO:0030544 | Hsp70 protein binding |
| Function | GO:0003723 | RNA binding |
| Process | GO:0009408 | response to heat |
| Process | GO:0009410 | response to xenobiotic stimulus |
Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):
| Stage | LR class | MCA mean | MCA prop. zeros |
|---|---|---|---|
| Sporozoite | expressed | N/A | N/A |
| Ring | expressed | 1.98 | 0.25 |
| Trophozoite | expressed | 2.15 | 0.16 |
| Schizont | expressed | 0.47 | 0.74 |
| Gametocyte | expressed | 1.51 | 0.30 |
More info:
Old (Pf3D7v3) Gene ID: PF3D7_1437900
Resistome Missense Mutations: None
Resistome Compounds with Missense Mutations: None
Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)
Zhang Phenotype: Non - Mutable in CDS
MIS: 0.814 | MFS: -2.425 | #Insertions: 0
PlasmoGEM Phenotype: N/A
RMgmDB ABS Phenotype: N/A
More info: PhenoPlasm Link
AlphaFill Uniprot ID: Q8IL88
"Best" AlphaFill ligand hit: ATP (adenosine-5'-triphosphate, Local RMSD=2.27) with 4WB7 (Global RMSD=20.49)
No associated EC numbersOrthology to BindingDB Entries:
| UniProt | Protein Name | Species | Homology Source | BindingDB Ligands |
|---|---|---|---|---|
| P31689 | DnaJ homolog subfamily A member 1 | Homo sapiens | OrthoDB | CHEMBL52073CHEMBL159171 |
Ortholog Group (OrthoMCL): OG6_100614
Most Similar Human Ortholog: I3L320
TM-align score: 0.71 | RMSD: 2.28
Seq Identity: 0.41 | Length: 41 / 424
All Human Orthologs (OrthoMCL):
| Gene ID | Description |
|---|---|
| ENSG00000069345 | DnaJ heat shock protein family (Hsp40) member A2 |
| ENSG00000086061 | DnaJ heat shock protein family (Hsp40) member A1 |
| ENSG00000140403 | DnaJ heat shock protein family (Hsp40) member A4 |
Protein Length: 424 | Molecular Weight (kDa): 48.469
UniProt ID(s): Q8IL88
PDB ID(s): None
Isoelectric Point: 7.15
Protein Domain Annotations:
| Source | Family ID | Description |
|---|---|---|
| InterPro | IPR001305 | Heat shock protein DnaJ, cysteine-rich domain |
| InterPro | IPR001623 | DnaJ domain |
| InterPro | IPR002939 | Chaperone DnaJ, C-terminal |
| InterPro | IPR008971 | HSP40/DnaJ peptide-binding |
| InterPro | IPR018253 | DnaJ domain, conserved site |
| InterPro | IPR036410 | Heat shock protein DnaJ, cysteine-rich domain superfamily |
| InterPro | IPR036869 | Chaperone J-domain superfamily |
| PFam | PF00226 | DnaJ domain |
| PFam | PF00684 | Heat shock protein DnaJ, cysteine-rich domain |
| PFam | PF01556 | Chaperone DnaJ, C-terminal |
| Superfamily | SSF46565 | Chaperone J-domain superfamily |
| Superfamily | SSF49493 | HSP40/DnaJ peptide-binding |
| Superfamily | SSF57938 | Heat shock protein DnaJ, cysteine-rich domain superfamily |
MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:
No passing variants found
PlasmoDB Total SNPs: 204
Non-coding: 190 | Synonymous: 10 | Nonsynonymous: 3 | Stop Codon: 1
| PMID | Title | Authors | DOI/Link |
|---|---|---|---|
| 12368864 | Genome sequence of the human malaria parasite Plasmodium falciparum. | Gardner MJ, Hall N, ..., Barrell B | 10.1038/nature01097 |
| 16267556 | A protein interaction network of the malaria parasite Plasmodium falciparum. | LaCount DJ, Vignali M, ..., Hughes RE | 10.1038/nature04104 |
| 21191678 | Plasmodium falciparum encodes a single cytosolic type I Hsp40 that functionallyinteracts with Hsp70 and is upregulated by heat shock. | Botha M, Chiang AN, ..., Blatch GL | 10.1007/s12192-010-0250-6 |
| 30379851 | Identification of Plasmodium falciparum nuclear proteins by mass spectrometry andproposed protein annotation. | Briquet S, Ourimi A, ..., Vaquero C | 10.1371/journal.pone.0205596 |
| 33906926 | Functional Characterization of the m(6)A-Dependent Translational Modulator PfYTH | Sinha A, Baumgarten S, ..., Scherf A | 10.1128/mBio.00661-21 |
| 34182772 | Protein Prenylation and Hsp40 in Thermotolerance of Plasmodium falciparum MalariaParasites. | Mathews ES, Jezewski AJ, Odom John AR | 10.1128/mBio.00760-21 |
| 38418371 | Plasmodium falciparum J-dot localized J domain protein A8iJp modulates thechaperone activity of human HSPA8 | Sahu W, Bai T, ..., Reddy KS | 10.1002/1873-3468.14836 |
| 38377589 | The Regulatory Effect of Huangshui Polysaccharides on Intestinal Microbiota andMetabolites during In Vitro Fermentation | Li M, Su J, ..., Liang H | 10.1021/acs.jafc.3c08658 |